Improved split-ubiquitin screening technique to identify surface membrane protein-protein interactions

Improved split-ubiquitin screening technique to identify surface membrane protein-protein interactions

Yeast-based methods are still the workhorse for the detection of protein-protein interactions (PPIs) in vivo. Yeast two-hybrid (Y2H) systems, however, are limited to screening for a specific group of molecules that interact in a particular cell compartment. For this reason, the split-ubiquitin syste...

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Bibliographic Details
Main Authors: Daniel Ivanusic, Jürgen J. Heinisch, Magdalena Eschricht, Ulrike Laube, Joachim Denner
Format: Article
Language:English
Published: Taylor & Francis Group 2015-08-01
Series:BioTechniques
Subjects:
protein-protein interactions
split-ubiquitin system
receptors
Online Access:https://www.future-science.com/doi/10.2144/000114315
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https://www.future-science.com/doi/10.2144/000114315

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