Genetic Polymorphism of Interferon-Gamma +874T/A as a Risk Factor for Pulmonary Tuberculosis in Cirebon, Indonesia
Background: Tuberculosis (TB) is one of the leading causes of death globally, caused by Mycobacterium tuberculosis. With 10% of all cases worldwide in 2022, Indonesia is the second-largest contributor of tuberculosis cases. IFN-γ gene polymorphism is one of the factors that have been studied extens...
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Yayasan Aliansi Cendekiawan Indonesia Thailand
2024-11-01
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| Series: | GHMJ (Global Health Management Journal) |
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| Online Access: | https://publications.inschool.id/index.php/ghmj/article/view/1107 |
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| author | Allain Umadela Deuxawalu Kiteswara Ariestya Indah Permata Sari Tiar Masykuroh Pratamawati |
| author_facet | Allain Umadela Deuxawalu Kiteswara Ariestya Indah Permata Sari Tiar Masykuroh Pratamawati |
| author_sort | Allain Umadela Deuxawalu Kiteswara |
| collection | DOAJ |
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Background: Tuberculosis (TB) is one of the leading causes of death globally, caused by Mycobacterium tuberculosis. With 10% of all cases worldwide in 2022, Indonesia is the second-largest contributor of tuberculosis cases. IFN-γ gene polymorphism is one of the factors that have been studied extensively for its association with TB.
Aims: To analyze IFN-γ +874T/A gene polymorphism as a risk factor for pulmonary tuberculosis in Cirebon.
Methods: Observational analysis with case control design was used in this study. Thirty-two tuberculosis patients as cases and 32 healthy controls at RSUD Waled were collected and performed DNA extraction to evaluate the polymorphism by using Amplification-refractory mutation system–polymerase chain reaction (ARMS–PCR). Statistical comparison was performed by using Pearson Chi-square and Kruskal Wallis test. Mann-Whitney U test was done for post hoc test. Odds ratio was calculated to see the risk of the assessed variables, including genotype, allele frequency, and the presence of polymorphism.
Results: In the case group, the frequency of TT genotype was 3 (9.4%), TA genotype was 26 (81.3%), AA genotype was 3 (9.4%). In the control group, the frequency of TT genotype was 12 (37.5%), TA genotype was 17 (53.1%), AA genotype was 3 (9.4%). A significant difference (p=0.034) was found among 3 genotype groups. Post hoc test revealed that TT and TA was the pair with significant difference (p=0.007). In addition, TA polymorphism was significantly associated (p=0.004) with tuberculosis (OR=6.614; CI95% = 1.660-26.349).
Conclusion: IFN-γ +874 TA gene polymorphism is associated with pulmonary tuberculosis in the population of Cirebon, West Java, Indonesia.
Received: 25 September 2024 | Reviewed: 23 October 2024 |
Revised: 10 November 2024 | Accepted: 30 November 2024
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| format | Article |
| id | doaj-art-670fd50d6a594c37b8bf3e7dca7e9f8a |
| institution | OA Journals |
| issn | 2580-9296 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Yayasan Aliansi Cendekiawan Indonesia Thailand |
| record_format | Article |
| series | GHMJ (Global Health Management Journal) |
| spelling | doaj-art-670fd50d6a594c37b8bf3e7dca7e9f8a2025-08-20T02:36:03ZengYayasan Aliansi Cendekiawan Indonesia ThailandGHMJ (Global Health Management Journal)2580-92962024-11-017410.35898/ghmj-741107Genetic Polymorphism of Interferon-Gamma +874T/A as a Risk Factor for Pulmonary Tuberculosis in Cirebon, IndonesiaAllain Umadela Deuxawalu Kiteswara0https://orcid.org/0009-0007-0686-7245Ariestya Indah Permata Sari1https://orcid.org/0000-0003-4037-937XTiar Masykuroh Pratamawati2https://orcid.org/0000-0002-2844-9307Department of Genetics, Faculty of Medicine, Universitas Swadaya Gunung Jati, Indonesia.Department of Genetics, Faculty of Medicine, Universitas Swadaya Gunung Jati, Indonesia.Department of Genetics, Faculty of Medicine, Universitas Swadaya Gunung Jati, Indonesia. Background: Tuberculosis (TB) is one of the leading causes of death globally, caused by Mycobacterium tuberculosis. With 10% of all cases worldwide in 2022, Indonesia is the second-largest contributor of tuberculosis cases. IFN-γ gene polymorphism is one of the factors that have been studied extensively for its association with TB. Aims: To analyze IFN-γ +874T/A gene polymorphism as a risk factor for pulmonary tuberculosis in Cirebon. Methods: Observational analysis with case control design was used in this study. Thirty-two tuberculosis patients as cases and 32 healthy controls at RSUD Waled were collected and performed DNA extraction to evaluate the polymorphism by using Amplification-refractory mutation system–polymerase chain reaction (ARMS–PCR). Statistical comparison was performed by using Pearson Chi-square and Kruskal Wallis test. Mann-Whitney U test was done for post hoc test. Odds ratio was calculated to see the risk of the assessed variables, including genotype, allele frequency, and the presence of polymorphism. Results: In the case group, the frequency of TT genotype was 3 (9.4%), TA genotype was 26 (81.3%), AA genotype was 3 (9.4%). In the control group, the frequency of TT genotype was 12 (37.5%), TA genotype was 17 (53.1%), AA genotype was 3 (9.4%). A significant difference (p=0.034) was found among 3 genotype groups. Post hoc test revealed that TT and TA was the pair with significant difference (p=0.007). In addition, TA polymorphism was significantly associated (p=0.004) with tuberculosis (OR=6.614; CI95% = 1.660-26.349). Conclusion: IFN-γ +874 TA gene polymorphism is associated with pulmonary tuberculosis in the population of Cirebon, West Java, Indonesia. Received: 25 September 2024 | Reviewed: 23 October 2024 | Revised: 10 November 2024 | Accepted: 30 November 2024 https://publications.inschool.id/index.php/ghmj/article/view/1107PolymorphismInterferon γInterferon-gammaTuberculosis |
| spellingShingle | Allain Umadela Deuxawalu Kiteswara Ariestya Indah Permata Sari Tiar Masykuroh Pratamawati Genetic Polymorphism of Interferon-Gamma +874T/A as a Risk Factor for Pulmonary Tuberculosis in Cirebon, Indonesia GHMJ (Global Health Management Journal) Polymorphism Interferon γ Interferon-gamma Tuberculosis |
| title | Genetic Polymorphism of Interferon-Gamma +874T/A as a Risk Factor for Pulmonary Tuberculosis in Cirebon, Indonesia |
| title_full | Genetic Polymorphism of Interferon-Gamma +874T/A as a Risk Factor for Pulmonary Tuberculosis in Cirebon, Indonesia |
| title_fullStr | Genetic Polymorphism of Interferon-Gamma +874T/A as a Risk Factor for Pulmonary Tuberculosis in Cirebon, Indonesia |
| title_full_unstemmed | Genetic Polymorphism of Interferon-Gamma +874T/A as a Risk Factor for Pulmonary Tuberculosis in Cirebon, Indonesia |
| title_short | Genetic Polymorphism of Interferon-Gamma +874T/A as a Risk Factor for Pulmonary Tuberculosis in Cirebon, Indonesia |
| title_sort | genetic polymorphism of interferon gamma 874t a as a risk factor for pulmonary tuberculosis in cirebon indonesia |
| topic | Polymorphism Interferon γ Interferon-gamma Tuberculosis |
| url | https://publications.inschool.id/index.php/ghmj/article/view/1107 |
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