Diosmetin Delays In Vitro Aging of Porcine Oocytes by Improving Mitochondrial Function and Reducing Oxidative Stress
Oocyte quality is crucial for successful fertilization and subsequent embryonic development. Post-ovulatory aging leads to reduced oocyte quality and impaired embryogenesis, representing an unavoidable challenge in terms of certain assisted reproductive techniques. Diosmetin (DIOS), a natural flavon...
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2025-01-01
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| author | Jia-Jun Ren Xiu-Wen Yuan Yu-Hao Zhang Zi-Long Meng Xing-Wei Liang Nam-Hyung Kim Yong-Nan Xu Ying-Hua Li |
| author_facet | Jia-Jun Ren Xiu-Wen Yuan Yu-Hao Zhang Zi-Long Meng Xing-Wei Liang Nam-Hyung Kim Yong-Nan Xu Ying-Hua Li |
| author_sort | Jia-Jun Ren |
| collection | DOAJ |
| description | Oocyte quality is crucial for successful fertilization and subsequent embryonic development. Post-ovulatory aging leads to reduced oocyte quality and impaired embryogenesis, representing an unavoidable challenge in terms of certain assisted reproductive techniques. Diosmetin (DIOS), a natural flavonoid found in lemons, spearmint, and spider moss, exhibits antioxidant, anti-inflammatory, and anti-apoptotic properties. However, its effects on the aging of mature porcine oocytes in vitro remain unexplored. This study investigated the impact of DIOS on porcine oocyte aging. In the IVM medium, fresh oocytes were cultured for 44 h, while aging oocytes were cultured for 68 h. Following the addition of varying DIOS concentrations (0.01, 0.1, and 1 μM) to the IVM medium, the DIOS-treated aging oocyte group was cultured for 68 h. The results demonstrated that 0.1 μM DIOS significantly improved the blastocyst rates and cell counts, reduced the reactive oxygen species (ROS), elevated the glutathione (GSH) levels, enhanced the mitochondrial function, and decreased the markers of autophagy (LC3B), apoptosis (annexin V), endoplasmic reticulum stress (CHOP), and senescence (SA-β-Gal). Furthermore, DIOS treatment upregulated the expression of relevant genes compared to the aged group. These findings suggest that DIOS effectively delays porcine oocyte aging. |
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| spelling | doaj-art-6703b60dc56b40f5b4fc2bbd209679152025-08-20T02:48:01ZengMDPI AGAnimals2076-26152025-01-0115329110.3390/ani15030291Diosmetin Delays In Vitro Aging of Porcine Oocytes by Improving Mitochondrial Function and Reducing Oxidative StressJia-Jun Ren0Xiu-Wen Yuan1Yu-Hao Zhang2Zi-Long Meng3Xing-Wei Liang4Nam-Hyung Kim5Yong-Nan Xu6Ying-Hua Li7Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529000, ChinaGuangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529000, ChinaGuangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529000, ChinaGuangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529000, ChinaCollege of Animal Science & Technology, Guangxi University, Nanning 530004, ChinaGuangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529000, ChinaGuangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529000, ChinaGuangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529000, ChinaOocyte quality is crucial for successful fertilization and subsequent embryonic development. Post-ovulatory aging leads to reduced oocyte quality and impaired embryogenesis, representing an unavoidable challenge in terms of certain assisted reproductive techniques. Diosmetin (DIOS), a natural flavonoid found in lemons, spearmint, and spider moss, exhibits antioxidant, anti-inflammatory, and anti-apoptotic properties. However, its effects on the aging of mature porcine oocytes in vitro remain unexplored. This study investigated the impact of DIOS on porcine oocyte aging. In the IVM medium, fresh oocytes were cultured for 44 h, while aging oocytes were cultured for 68 h. Following the addition of varying DIOS concentrations (0.01, 0.1, and 1 μM) to the IVM medium, the DIOS-treated aging oocyte group was cultured for 68 h. The results demonstrated that 0.1 μM DIOS significantly improved the blastocyst rates and cell counts, reduced the reactive oxygen species (ROS), elevated the glutathione (GSH) levels, enhanced the mitochondrial function, and decreased the markers of autophagy (LC3B), apoptosis (annexin V), endoplasmic reticulum stress (CHOP), and senescence (SA-β-Gal). Furthermore, DIOS treatment upregulated the expression of relevant genes compared to the aged group. These findings suggest that DIOS effectively delays porcine oocyte aging.https://www.mdpi.com/2076-2615/15/3/291diosmetinautophagyapoptosismitochondrialoocyte aging |
| spellingShingle | Jia-Jun Ren Xiu-Wen Yuan Yu-Hao Zhang Zi-Long Meng Xing-Wei Liang Nam-Hyung Kim Yong-Nan Xu Ying-Hua Li Diosmetin Delays In Vitro Aging of Porcine Oocytes by Improving Mitochondrial Function and Reducing Oxidative Stress Animals diosmetin autophagy apoptosis mitochondrial oocyte aging |
| title | Diosmetin Delays In Vitro Aging of Porcine Oocytes by Improving Mitochondrial Function and Reducing Oxidative Stress |
| title_full | Diosmetin Delays In Vitro Aging of Porcine Oocytes by Improving Mitochondrial Function and Reducing Oxidative Stress |
| title_fullStr | Diosmetin Delays In Vitro Aging of Porcine Oocytes by Improving Mitochondrial Function and Reducing Oxidative Stress |
| title_full_unstemmed | Diosmetin Delays In Vitro Aging of Porcine Oocytes by Improving Mitochondrial Function and Reducing Oxidative Stress |
| title_short | Diosmetin Delays In Vitro Aging of Porcine Oocytes by Improving Mitochondrial Function and Reducing Oxidative Stress |
| title_sort | diosmetin delays in vitro aging of porcine oocytes by improving mitochondrial function and reducing oxidative stress |
| topic | diosmetin autophagy apoptosis mitochondrial oocyte aging |
| url | https://www.mdpi.com/2076-2615/15/3/291 |
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