Influence of Surface Material and Nutrient Conditions on Green Fluorescent Protein Production in <i>Escherichia coli</i> Biofilms
<i>Escherichia coli</i> biofilms have been investigated as a platform for producing recombinant proteins. This study aimed to assess the effect of different surface materials and culture media on <i>E. coli</i> biofilm formation and enhanced Green Fluorescent Protein (eGFP) p...
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MDPI AG
2024-11-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/14/23/11029 |
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| author | Ana Azevedo Rita Teixeira-Santos Fábio M. Carvalho Luciana C. Gomes Gabriel A. Monteiro Filipe J. Mergulhão |
| author_facet | Ana Azevedo Rita Teixeira-Santos Fábio M. Carvalho Luciana C. Gomes Gabriel A. Monteiro Filipe J. Mergulhão |
| author_sort | Ana Azevedo |
| collection | DOAJ |
| description | <i>Escherichia coli</i> biofilms have been investigated as a platform for producing recombinant proteins. This study aimed to assess the effect of different surface materials and culture media on <i>E. coli</i> biofilm formation and enhanced Green Fluorescent Protein (eGFP) production. Three culture media with different carbon and nitrogen sources (Lysogeny broth, Terrific broth, and M9ZB broth) were tested in combination with three materials with distinct surface properties (stainless steel, polyvinyl chloride, and silicone rubber). Biofilm formation, specific eGFP production, and plasmid copy number were monitored in microtiter plates for 9 days. Microscopy and culturability results indicated that biofilm formation was highest in Terrific broth, regardless of the surface material. Additionally, polyvinyl chloride surfaces exposed to Terrific broth provided the most advantageous conditions for achieving the highest specific eGFP production and plasmid maintenance in biofilms. These findings are relevant for establishing operational conditions for producing recombinant proteins and other high-value-added compounds on larger-scale biofilm platforms. |
| format | Article |
| id | doaj-art-6702a38dbf5e4fe1865e5ba9f9b87c46 |
| institution | Kabale University |
| issn | 2076-3417 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj-art-6702a38dbf5e4fe1865e5ba9f9b87c462024-12-13T16:22:35ZengMDPI AGApplied Sciences2076-34172024-11-0114231102910.3390/app142311029Influence of Surface Material and Nutrient Conditions on Green Fluorescent Protein Production in <i>Escherichia coli</i> BiofilmsAna Azevedo0Rita Teixeira-Santos1Fábio M. Carvalho2Luciana C. Gomes3Gabriel A. Monteiro4Filipe J. Mergulhão5LEPABE—Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, PortugalLEPABE—Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, PortugalLEPABE—Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, PortugalLEPABE—Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, PortugaliBB—Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, University of Lisbon, 1049-001 Lisboa, PortugalLEPABE—Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal<i>Escherichia coli</i> biofilms have been investigated as a platform for producing recombinant proteins. This study aimed to assess the effect of different surface materials and culture media on <i>E. coli</i> biofilm formation and enhanced Green Fluorescent Protein (eGFP) production. Three culture media with different carbon and nitrogen sources (Lysogeny broth, Terrific broth, and M9ZB broth) were tested in combination with three materials with distinct surface properties (stainless steel, polyvinyl chloride, and silicone rubber). Biofilm formation, specific eGFP production, and plasmid copy number were monitored in microtiter plates for 9 days. Microscopy and culturability results indicated that biofilm formation was highest in Terrific broth, regardless of the surface material. Additionally, polyvinyl chloride surfaces exposed to Terrific broth provided the most advantageous conditions for achieving the highest specific eGFP production and plasmid maintenance in biofilms. These findings are relevant for establishing operational conditions for producing recombinant proteins and other high-value-added compounds on larger-scale biofilm platforms.https://www.mdpi.com/2076-3417/14/23/11029heterologous protein expressionenhanced Green Fluorescent Proteinbiofilm<i>Escherichia coli</i>surface propertiesnutritional conditions |
| spellingShingle | Ana Azevedo Rita Teixeira-Santos Fábio M. Carvalho Luciana C. Gomes Gabriel A. Monteiro Filipe J. Mergulhão Influence of Surface Material and Nutrient Conditions on Green Fluorescent Protein Production in <i>Escherichia coli</i> Biofilms Applied Sciences heterologous protein expression enhanced Green Fluorescent Protein biofilm <i>Escherichia coli</i> surface properties nutritional conditions |
| title | Influence of Surface Material and Nutrient Conditions on Green Fluorescent Protein Production in <i>Escherichia coli</i> Biofilms |
| title_full | Influence of Surface Material and Nutrient Conditions on Green Fluorescent Protein Production in <i>Escherichia coli</i> Biofilms |
| title_fullStr | Influence of Surface Material and Nutrient Conditions on Green Fluorescent Protein Production in <i>Escherichia coli</i> Biofilms |
| title_full_unstemmed | Influence of Surface Material and Nutrient Conditions on Green Fluorescent Protein Production in <i>Escherichia coli</i> Biofilms |
| title_short | Influence of Surface Material and Nutrient Conditions on Green Fluorescent Protein Production in <i>Escherichia coli</i> Biofilms |
| title_sort | influence of surface material and nutrient conditions on green fluorescent protein production in i escherichia coli i biofilms |
| topic | heterologous protein expression enhanced Green Fluorescent Protein biofilm <i>Escherichia coli</i> surface properties nutritional conditions |
| url | https://www.mdpi.com/2076-3417/14/23/11029 |
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