Morphological Analysis of US Treated PANC-1 Spheroids
This study investigates the impact of low-intensity continuous ultrasound (LICU) on pancreatic adenocarcinoma (PANC-1) spheroids, emphasizing morphological and volumetric transformations. PANC-1 spheroids were cultured and treated with LICU across frequencies from 1 to 5 MHz. Cell viability and mort...
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| Language: | English |
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MDPI AG
2025-02-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/15/4/1707 |
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| author | Martina Ricci Mattia Dimitri Martina Serio Andrea Corvi |
| author_facet | Martina Ricci Mattia Dimitri Martina Serio Andrea Corvi |
| author_sort | Martina Ricci |
| collection | DOAJ |
| description | This study investigates the impact of low-intensity continuous ultrasound (LICU) on pancreatic adenocarcinoma (PANC-1) spheroids, emphasizing morphological and volumetric transformations. PANC-1 spheroids were cultured and treated with LICU across frequencies from 1 to 5 MHz. Cell viability and mortality were analyzed through Calcein AM/PI staining, while volumetric and morphological changes were quantified across frequencies from 2 to 4 MHz using advanced imaging techniques and computational tools, including a custom Python OpenCv Library, AnaSP 3.0, a MATLAB based open source tool. Notably, a frequency of 3.5 MHz yielded optimal outcomes, also achieving a reduction in spheroid volume and mortality while minimizing disgregation, a factor linked to metastasis risk. These findings underscore LICU’s potential as an effective therapeutic strategy, balancing tumor reduction with the preservation of structural cohesion. The study establishes a methodological framework for optimizing LICU parameters, presenting a less invasive avenue for improving therapeutic outcomes in pancreatic cancer treatment. |
| format | Article |
| id | doaj-art-66f7ccfd02584c6e884eab1f49a12e8b |
| institution | DOAJ |
| issn | 2076-3417 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj-art-66f7ccfd02584c6e884eab1f49a12e8b2025-08-20T02:44:36ZengMDPI AGApplied Sciences2076-34172025-02-01154170710.3390/app15041707Morphological Analysis of US Treated PANC-1 SpheroidsMartina Ricci0Mattia Dimitri1Martina Serio2Andrea Corvi3Department of Industrial Engineering (DIEF), University of Florence, Via di Santa Marta 3, 50139 Florence, ItalyDepartment of Industrial Engineering (DIEF), University of Florence, Via di Santa Marta 3, 50139 Florence, ItalyDepartment of Industrial Engineering (DIEF), University of Florence, Via di Santa Marta 3, 50139 Florence, ItalyDepartment of Industrial Engineering (DIEF), University of Florence, Via di Santa Marta 3, 50139 Florence, ItalyThis study investigates the impact of low-intensity continuous ultrasound (LICU) on pancreatic adenocarcinoma (PANC-1) spheroids, emphasizing morphological and volumetric transformations. PANC-1 spheroids were cultured and treated with LICU across frequencies from 1 to 5 MHz. Cell viability and mortality were analyzed through Calcein AM/PI staining, while volumetric and morphological changes were quantified across frequencies from 2 to 4 MHz using advanced imaging techniques and computational tools, including a custom Python OpenCv Library, AnaSP 3.0, a MATLAB based open source tool. Notably, a frequency of 3.5 MHz yielded optimal outcomes, also achieving a reduction in spheroid volume and mortality while minimizing disgregation, a factor linked to metastasis risk. These findings underscore LICU’s potential as an effective therapeutic strategy, balancing tumor reduction with the preservation of structural cohesion. The study establishes a methodological framework for optimizing LICU parameters, presenting a less invasive avenue for improving therapeutic outcomes in pancreatic cancer treatment.https://www.mdpi.com/2076-3417/15/4/1707LICUvolume evaluationUS cancer therapycomputer visionspheroids disgregationfrequency induced mortality |
| spellingShingle | Martina Ricci Mattia Dimitri Martina Serio Andrea Corvi Morphological Analysis of US Treated PANC-1 Spheroids Applied Sciences LICU volume evaluation US cancer therapy computer vision spheroids disgregation frequency induced mortality |
| title | Morphological Analysis of US Treated PANC-1 Spheroids |
| title_full | Morphological Analysis of US Treated PANC-1 Spheroids |
| title_fullStr | Morphological Analysis of US Treated PANC-1 Spheroids |
| title_full_unstemmed | Morphological Analysis of US Treated PANC-1 Spheroids |
| title_short | Morphological Analysis of US Treated PANC-1 Spheroids |
| title_sort | morphological analysis of us treated panc 1 spheroids |
| topic | LICU volume evaluation US cancer therapy computer vision spheroids disgregation frequency induced mortality |
| url | https://www.mdpi.com/2076-3417/15/4/1707 |
| work_keys_str_mv | AT martinaricci morphologicalanalysisofustreatedpanc1spheroids AT mattiadimitri morphologicalanalysisofustreatedpanc1spheroids AT martinaserio morphologicalanalysisofustreatedpanc1spheroids AT andreacorvi morphologicalanalysisofustreatedpanc1spheroids |