Development and Validation of UV-Visible Spectrophotometric Baseline Manipulation Method for Simultaneous Quantitation of Tenofovir Disoproxil Fumarate and Emtricitabine in Pharmaceutical Dosage Form
A simple, economical, precise, and accurate new UV-visible spectrophotometric baseline manipulation method for simultaneous determination of tenofovir disoproxil fumarate (TE) and emtricitabine (EM) in combined tablet dosage form has been developed. The method is based on baseline manipulation (dif...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Spectroscopy |
| Online Access: | http://dx.doi.org/10.1155/2013/146580 |
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| author | Vishnu P. Choudhari Sanket R. Parekar Subhash G. Chate Pradeep D. Bharande Rajiv R. Singh Bhanudas S. Kuchekar |
| author_facet | Vishnu P. Choudhari Sanket R. Parekar Subhash G. Chate Pradeep D. Bharande Rajiv R. Singh Bhanudas S. Kuchekar |
| author_sort | Vishnu P. Choudhari |
| collection | DOAJ |
| description | A simple, economical, precise, and accurate new UV-visible spectrophotometric baseline manipulation method for simultaneous determination of tenofovir disoproxil fumarate (TE) and emtricitabine (EM) in combined tablet dosage form has been developed. The method is based on baseline manipulation (difference) spectroscopy where amplitudes at 261 and 289.9 nm were selected to determine TE and EM, respectively, in combined formulation, and distilled water was used as solvent. Both drugs obey Beer’s law in the concentration ranges of 4–20 μg/mL for TE and 6–30 μg/mL for EM. The results of analysis have been validated statistically, and recovery studies confirmed the accuracy of the proposed method which was carried out by following the ICH guidelines. |
| format | Article |
| id | doaj-art-66f0fb87092349de87e3250dade94aa6 |
| institution | Kabale University |
| issn | 2314-4920 2314-4939 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Spectroscopy |
| spelling | doaj-art-66f0fb87092349de87e3250dade94aa62025-02-03T06:05:07ZengWileyJournal of Spectroscopy2314-49202314-49392013-01-01201310.1155/2013/146580146580Development and Validation of UV-Visible Spectrophotometric Baseline Manipulation Method for Simultaneous Quantitation of Tenofovir Disoproxil Fumarate and Emtricitabine in Pharmaceutical Dosage FormVishnu P. Choudhari0Sanket R. Parekar1Subhash G. Chate2Pradeep D. Bharande3Rajiv R. Singh4Bhanudas S. Kuchekar5Pharmacy Department, Maharashtra Institute of Pharmacy, MIT Campus, Paud Road, Kothrud, Pune 411038, IndiaPharmacy Department, Maharashtra Institute of Pharmacy, MIT Campus, Paud Road, Kothrud, Pune 411038, IndiaPharmacy Department, Maharashtra Institute of Pharmacy, MIT Campus, Paud Road, Kothrud, Pune 411038, IndiaPharmacy Department, Maharashtra Institute of Pharmacy, MIT Campus, Paud Road, Kothrud, Pune 411038, IndiaPharmacy Department, Maharashtra Institute of Pharmacy, MIT Campus, Paud Road, Kothrud, Pune 411038, IndiaPharmacy Department, Maharashtra Institute of Pharmacy, MIT Campus, Paud Road, Kothrud, Pune 411038, IndiaA simple, economical, precise, and accurate new UV-visible spectrophotometric baseline manipulation method for simultaneous determination of tenofovir disoproxil fumarate (TE) and emtricitabine (EM) in combined tablet dosage form has been developed. The method is based on baseline manipulation (difference) spectroscopy where amplitudes at 261 and 289.9 nm were selected to determine TE and EM, respectively, in combined formulation, and distilled water was used as solvent. Both drugs obey Beer’s law in the concentration ranges of 4–20 μg/mL for TE and 6–30 μg/mL for EM. The results of analysis have been validated statistically, and recovery studies confirmed the accuracy of the proposed method which was carried out by following the ICH guidelines.http://dx.doi.org/10.1155/2013/146580 |
| spellingShingle | Vishnu P. Choudhari Sanket R. Parekar Subhash G. Chate Pradeep D. Bharande Rajiv R. Singh Bhanudas S. Kuchekar Development and Validation of UV-Visible Spectrophotometric Baseline Manipulation Method for Simultaneous Quantitation of Tenofovir Disoproxil Fumarate and Emtricitabine in Pharmaceutical Dosage Form Journal of Spectroscopy |
| title | Development and Validation of UV-Visible Spectrophotometric Baseline Manipulation Method for Simultaneous Quantitation of Tenofovir Disoproxil Fumarate and Emtricitabine in Pharmaceutical Dosage Form |
| title_full | Development and Validation of UV-Visible Spectrophotometric Baseline Manipulation Method for Simultaneous Quantitation of Tenofovir Disoproxil Fumarate and Emtricitabine in Pharmaceutical Dosage Form |
| title_fullStr | Development and Validation of UV-Visible Spectrophotometric Baseline Manipulation Method for Simultaneous Quantitation of Tenofovir Disoproxil Fumarate and Emtricitabine in Pharmaceutical Dosage Form |
| title_full_unstemmed | Development and Validation of UV-Visible Spectrophotometric Baseline Manipulation Method for Simultaneous Quantitation of Tenofovir Disoproxil Fumarate and Emtricitabine in Pharmaceutical Dosage Form |
| title_short | Development and Validation of UV-Visible Spectrophotometric Baseline Manipulation Method for Simultaneous Quantitation of Tenofovir Disoproxil Fumarate and Emtricitabine in Pharmaceutical Dosage Form |
| title_sort | development and validation of uv visible spectrophotometric baseline manipulation method for simultaneous quantitation of tenofovir disoproxil fumarate and emtricitabine in pharmaceutical dosage form |
| url | http://dx.doi.org/10.1155/2013/146580 |
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