Chromosome-scale haploid genome assembly of Durio zibethinus KanYao

Abstract Durian (Durio zibethinus) is a tropical fruit valued for its nutritional and commercial significance. In this study, we generated two high-quality, haplotype-resolved, chromosome-level genomes of the durian cultivar ‘Kan Yao’, each with 28 chromosomes. The total genome lengths were 737.2 Mb...

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Main Authors: Xiaohao Ji, Yiwang Zhong, Daojun Zheng, Shenghua Xie, Meng Shi, Xiaodi Wang, Fengzhi Liu, Xuejie Feng, Haibo Wang
Format: Article
Language:English
Published: Nature Portfolio 2025-03-01
Series:Scientific Data
Online Access:https://doi.org/10.1038/s41597-025-04656-y
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author Xiaohao Ji
Yiwang Zhong
Daojun Zheng
Shenghua Xie
Meng Shi
Xiaodi Wang
Fengzhi Liu
Xuejie Feng
Haibo Wang
author_facet Xiaohao Ji
Yiwang Zhong
Daojun Zheng
Shenghua Xie
Meng Shi
Xiaodi Wang
Fengzhi Liu
Xuejie Feng
Haibo Wang
author_sort Xiaohao Ji
collection DOAJ
description Abstract Durian (Durio zibethinus) is a tropical fruit valued for its nutritional and commercial significance. In this study, we generated two high-quality, haplotype-resolved, chromosome-level genomes of the durian cultivar ‘Kan Yao’, each with 28 chromosomes. The total genome lengths were 737.2 Mb and 763.8 Mb, with contig N50 values of 22.9 Mb and 21.5 Mb, and scaffold N50 values of 25.9 Mb and 26.7 Mb, respectively. Nineteen chromosomes were assembled without gaps, while the remaining nine contained 1 to 10 gaps. Genome annotation identified 53,125 and 53,101 functional genes, as well as 5,254 and 5,496 non-coding RNAs. The high-quality assembled genomes will aid in the molecular breeding of durian.
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spelling doaj-art-66ec4566e0794851904f6ac6b5dadbee2025-08-20T01:57:44ZengNature PortfolioScientific Data2052-44632025-03-0112111010.1038/s41597-025-04656-yChromosome-scale haploid genome assembly of Durio zibethinus KanYaoXiaohao Ji0Yiwang Zhong1Daojun Zheng2Shenghua Xie3Meng Shi4Xiaodi Wang5Fengzhi Liu6Xuejie Feng7Haibo Wang8National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural SciencesSanya Institute, Hainan Academy of Agricultural Sciences; Institute of Tropical Fruit Trees, Hainan Academy of Agricultural Sciences; Key Laboratory of Genetic Resources Evaluation and Utilization of Tropical Fruits and Vegetables (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs; Key Laboratory of Tropical Fruit Tree Biology of Hainan Province; Ministry of Agriculture and Rural Affairs/Haikou Scientific Observation and Experimental Station for Tropical Fruit Trees, Hainan Field Scientific Observation and Research Station for Tropical Fruit TreesSanya Institute, Hainan Academy of Agricultural Sciences; Institute of Tropical Fruit Trees, Hainan Academy of Agricultural Sciences; Key Laboratory of Genetic Resources Evaluation and Utilization of Tropical Fruits and Vegetables (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs; Key Laboratory of Tropical Fruit Tree Biology of Hainan Province; Ministry of Agriculture and Rural Affairs/Haikou Scientific Observation and Experimental Station for Tropical Fruit Trees, Hainan Field Scientific Observation and Research Station for Tropical Fruit TreesSanya Institute, Hainan Academy of Agricultural Sciences; Institute of Tropical Fruit Trees, Hainan Academy of Agricultural Sciences; Key Laboratory of Genetic Resources Evaluation and Utilization of Tropical Fruits and Vegetables (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs; Key Laboratory of Tropical Fruit Tree Biology of Hainan Province; Ministry of Agriculture and Rural Affairs/Haikou Scientific Observation and Experimental Station for Tropical Fruit Trees, Hainan Field Scientific Observation and Research Station for Tropical Fruit TreesResearch Institute of Pomology, Chinese Academy of Agricultural Sciences/Key Laboratory of Horticultural Crops Germplasm Resources Utilization, Ministry of Agriculture and Rural AffairsResearch Institute of Pomology, Chinese Academy of Agricultural Sciences/Key Laboratory of Horticultural Crops Germplasm Resources Utilization, Ministry of Agriculture and Rural AffairsResearch Institute of Pomology, Chinese Academy of Agricultural Sciences/Key Laboratory of Horticultural Crops Germplasm Resources Utilization, Ministry of Agriculture and Rural AffairsSanya Institute, Hainan Academy of Agricultural Sciences; Institute of Tropical Fruit Trees, Hainan Academy of Agricultural Sciences; Key Laboratory of Genetic Resources Evaluation and Utilization of Tropical Fruits and Vegetables (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs; Key Laboratory of Tropical Fruit Tree Biology of Hainan Province; Ministry of Agriculture and Rural Affairs/Haikou Scientific Observation and Experimental Station for Tropical Fruit Trees, Hainan Field Scientific Observation and Research Station for Tropical Fruit TreesNational Nanfan Research Institute (Sanya), Chinese Academy of Agricultural SciencesAbstract Durian (Durio zibethinus) is a tropical fruit valued for its nutritional and commercial significance. In this study, we generated two high-quality, haplotype-resolved, chromosome-level genomes of the durian cultivar ‘Kan Yao’, each with 28 chromosomes. The total genome lengths were 737.2 Mb and 763.8 Mb, with contig N50 values of 22.9 Mb and 21.5 Mb, and scaffold N50 values of 25.9 Mb and 26.7 Mb, respectively. Nineteen chromosomes were assembled without gaps, while the remaining nine contained 1 to 10 gaps. Genome annotation identified 53,125 and 53,101 functional genes, as well as 5,254 and 5,496 non-coding RNAs. The high-quality assembled genomes will aid in the molecular breeding of durian.https://doi.org/10.1038/s41597-025-04656-y
spellingShingle Xiaohao Ji
Yiwang Zhong
Daojun Zheng
Shenghua Xie
Meng Shi
Xiaodi Wang
Fengzhi Liu
Xuejie Feng
Haibo Wang
Chromosome-scale haploid genome assembly of Durio zibethinus KanYao
Scientific Data
title Chromosome-scale haploid genome assembly of Durio zibethinus KanYao
title_full Chromosome-scale haploid genome assembly of Durio zibethinus KanYao
title_fullStr Chromosome-scale haploid genome assembly of Durio zibethinus KanYao
title_full_unstemmed Chromosome-scale haploid genome assembly of Durio zibethinus KanYao
title_short Chromosome-scale haploid genome assembly of Durio zibethinus KanYao
title_sort chromosome scale haploid genome assembly of durio zibethinus kanyao
url https://doi.org/10.1038/s41597-025-04656-y
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