Cold Stress Regulates Muscle Development and Promotes Muscle Fiber Transformation by Regulating Mib1/Notch Pathway
Background: In mammals, skeletal muscle typically constitutes approximately 55% of body weight. The thermogenesis of skeletal muscle increases with increased cold stress, and skeletal muscle maintains the animal’s body temperature through the heat generated by shivering. However,...
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IMR Press
2025-07-01
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| Series: | Frontiers in Bioscience-Landmark |
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| Online Access: | https://www.imrpress.com/journal/FBL/30/7/10.31083/FBL40141 |
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| author | Minxing Zheng Jiahui Qi Xuanjing Wang Tingting Fu Ziqi Chang Tong Zhao Yaqin Sun Jiayin Lu Yi Yan Haidong Wang |
| author_facet | Minxing Zheng Jiahui Qi Xuanjing Wang Tingting Fu Ziqi Chang Tong Zhao Yaqin Sun Jiayin Lu Yi Yan Haidong Wang |
| author_sort | Minxing Zheng |
| collection | DOAJ |
| description | Background: In mammals, skeletal muscle typically constitutes approximately 55% of body weight. The thermogenesis of skeletal muscle increases with increased cold stress, and skeletal muscle maintains the animal’s body temperature through the heat generated by shivering. However, less attention has been paid to investigating the impact of cold stress on the fiber type makeup of skeletal muscle, especially the gastrocnemius. Consequently, this research explored how cold stress regulates muscle development and fiber type composition. Methods: A cold stress model was established by subjecting mice to a 4 °C environment for 4 hours daily. This model was combined with an in vitro siRNA-mediated knockdown model for joint validation. The impact of cold stress on skeletal muscle development and myofiber type transformation was assessed using experimental techniques, including immunofluorescence and western blotting. Results: Following cold stress, the expression level of Myosin Heavy Chain 7 (MYH7) in the mouse gastrocnemius increased, while Myosin Heavy Chain 4 (MYH4) expression decreased. Concurrently, elevated expressions of Mindbomb-1 (Mib1) and the myogenic differentiation (MyoD) were observed. Subsequent knockdown of Mib1 in C2C12 cells resulted in increased MYH4 expression and decreased MYH7 expression. Conclusion: Cold stress induces skeletal muscle fibers to shift from fast-twitch to slow-twitch through the Mib1/Notch signaling pathway. |
| format | Article |
| id | doaj-art-66dbe90f37df499caeec908dabb4d2b4 |
| institution | DOAJ |
| issn | 2768-6701 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Frontiers in Bioscience-Landmark |
| spelling | doaj-art-66dbe90f37df499caeec908dabb4d2b42025-08-20T02:56:26ZengIMR PressFrontiers in Bioscience-Landmark2768-67012025-07-013074014110.31083/FBL40141S2768-6701(25)01780-0Cold Stress Regulates Muscle Development and Promotes Muscle Fiber Transformation by Regulating Mib1/Notch PathwayMinxing Zheng0Jiahui Qi1Xuanjing Wang2Tingting Fu3Ziqi Chang4Tong Zhao5Yaqin Sun6Jiayin Lu7Yi Yan8Haidong Wang9College of Veterinary Medicine, Shanxi Agricultural University, 030801 Jinzhong, Shanxi, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, 030801 Jinzhong, Shanxi, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, 030801 Jinzhong, Shanxi, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, 030801 Jinzhong, Shanxi, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, 030801 Jinzhong, Shanxi, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, 030801 Jinzhong, Shanxi, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, 030801 Jinzhong, Shanxi, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, 030801 Jinzhong, Shanxi, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, 030801 Jinzhong, Shanxi, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, 030801 Jinzhong, Shanxi, ChinaBackground: In mammals, skeletal muscle typically constitutes approximately 55% of body weight. The thermogenesis of skeletal muscle increases with increased cold stress, and skeletal muscle maintains the animal’s body temperature through the heat generated by shivering. However, less attention has been paid to investigating the impact of cold stress on the fiber type makeup of skeletal muscle, especially the gastrocnemius. Consequently, this research explored how cold stress regulates muscle development and fiber type composition. Methods: A cold stress model was established by subjecting mice to a 4 °C environment for 4 hours daily. This model was combined with an in vitro siRNA-mediated knockdown model for joint validation. The impact of cold stress on skeletal muscle development and myofiber type transformation was assessed using experimental techniques, including immunofluorescence and western blotting. Results: Following cold stress, the expression level of Myosin Heavy Chain 7 (MYH7) in the mouse gastrocnemius increased, while Myosin Heavy Chain 4 (MYH4) expression decreased. Concurrently, elevated expressions of Mindbomb-1 (Mib1) and the myogenic differentiation (MyoD) were observed. Subsequent knockdown of Mib1 in C2C12 cells resulted in increased MYH4 expression and decreased MYH7 expression. Conclusion: Cold stress induces skeletal muscle fibers to shift from fast-twitch to slow-twitch through the Mib1/Notch signaling pathway.https://www.imrpress.com/journal/FBL/30/7/10.31083/FBL40141cold stressmib1 genenotch signaling pathwayskeletal musclemuscle developmentmuscle fiber types |
| spellingShingle | Minxing Zheng Jiahui Qi Xuanjing Wang Tingting Fu Ziqi Chang Tong Zhao Yaqin Sun Jiayin Lu Yi Yan Haidong Wang Cold Stress Regulates Muscle Development and Promotes Muscle Fiber Transformation by Regulating Mib1/Notch Pathway Frontiers in Bioscience-Landmark cold stress mib1 gene notch signaling pathway skeletal muscle muscle development muscle fiber types |
| title | Cold Stress Regulates Muscle Development and Promotes Muscle Fiber Transformation by Regulating Mib1/Notch Pathway |
| title_full | Cold Stress Regulates Muscle Development and Promotes Muscle Fiber Transformation by Regulating Mib1/Notch Pathway |
| title_fullStr | Cold Stress Regulates Muscle Development and Promotes Muscle Fiber Transformation by Regulating Mib1/Notch Pathway |
| title_full_unstemmed | Cold Stress Regulates Muscle Development and Promotes Muscle Fiber Transformation by Regulating Mib1/Notch Pathway |
| title_short | Cold Stress Regulates Muscle Development and Promotes Muscle Fiber Transformation by Regulating Mib1/Notch Pathway |
| title_sort | cold stress regulates muscle development and promotes muscle fiber transformation by regulating mib1 notch pathway |
| topic | cold stress mib1 gene notch signaling pathway skeletal muscle muscle development muscle fiber types |
| url | https://www.imrpress.com/journal/FBL/30/7/10.31083/FBL40141 |
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