Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue
IntroductionThis study explores the effects of moderate-intensity exercise on protein lactylation in mouse muscle tissue metabolism.MethodsHealthy adult mice running for 6 weeks as an exercise model and sedentary mice as the control were used to perform transcriptomic, proteomic, lactylation-proteom...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2024.1472338/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832583785347022848 |
|---|---|
| author | Jiahui Chang Jiahui Chang Jiahui Chang Wanyu Wu Wanyu Wu Wanyu Wu Ping Qian Zhaoxu Lu Xuejia He Fang Wang Ting Zhang Ting Zhang Ting Zhang |
| author_facet | Jiahui Chang Jiahui Chang Jiahui Chang Wanyu Wu Wanyu Wu Wanyu Wu Ping Qian Zhaoxu Lu Xuejia He Fang Wang Ting Zhang Ting Zhang Ting Zhang |
| author_sort | Jiahui Chang |
| collection | DOAJ |
| description | IntroductionThis study explores the effects of moderate-intensity exercise on protein lactylation in mouse muscle tissue metabolism.MethodsHealthy adult mice running for 6 weeks as an exercise model and sedentary mice as the control were used to perform transcriptomic, proteomic, lactylation-proteomic, and metabolomic analyses. Correlation analysis between transcriptome and proteome and between proteome and metabolome was also conducted.ResultsIn this study, 159 lactylation sites of 78 proteins were identified as being differentially regulated by moderate-intensity exercise. Enrichment analysis showed that the lactylation of proteins Atp5mg, and Atp5po exhibited ATP hydrolysis activity. Mtatp8 and Atp5po were involved in biological processes such as mitochondrial transmembrane transport, and Mtatp8, Atp5mg, and Atp5po participate in oxidative phosphorylation and thermogenesis pathways. The lactylation levels of Mtatp8, Atp5mg, and Atp5po proteins in the exercise group were significantly decreased, while their protein levels were significantly increased. The combined analysis of proteomics and metabolomics showed that the oxocarboxylic acid metabolism and sphingolipid signaling pathways had significant changes under the influence of moderate-intensity exercise.DiscussionOur results indicate that moderate-intensity exercise has an effect on the lactylation level of mice, possibly by reducing the lactylation levels of Mtatp8, Atp5mg, and Atp5po and increasing the expression of their protein levels, thereby regulating the oxidative phosphorylation pathway and participating in energy metabolism. Further exploration is needed into the 2-oxocarboxylic acid metabolism pathway and the sphingolipid signaling pathway. |
| format | Article |
| id | doaj-art-66d9e53b34f84ae5b8ffed24af0758f4 |
| institution | Kabale University |
| issn | 2296-634X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj-art-66d9e53b34f84ae5b8ffed24af0758f42025-01-28T06:41:15ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2025-01-011210.3389/fcell.2024.14723381472338Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissueJiahui Chang0Jiahui Chang1Jiahui Chang2Wanyu Wu3Wanyu Wu4Wanyu Wu5Ping Qian6Zhaoxu Lu7Xuejia He8Fang Wang9Ting Zhang10Ting Zhang11Ting Zhang12Children’s Hospital Capital Institute of Pediatrics, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaBeijing Municipal Key Laboratory of Child Development and Nutriomics, Capital Institute of Pediatrics, Beijing, ChinaGraduate School of Peking Union Medical College, Beijing, ChinaChildren’s Hospital Capital Institute of Pediatrics, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaBeijing Municipal Key Laboratory of Child Development and Nutriomics, Capital Institute of Pediatrics, Beijing, ChinaGraduate School of Peking Union Medical College, Beijing, ChinaDepartment of Internet Medicine, Affiliated Children Hospital of Capital Institute of Pediatrics, Beijing, ChinaBeijing Municipal Key Laboratory of Child Development and Nutriomics, Capital Institute of Pediatrics, Beijing, ChinaBeijing Municipal Key Laboratory of Child Development and Nutriomics, Capital Institute of Pediatrics-Peking University Teaching Hospital, Beijing, ChinaBeijing Municipal Key Laboratory of Child Development and Nutriomics, Capital Institute of Pediatrics, Beijing, ChinaChildren’s Hospital Capital Institute of Pediatrics, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaBeijing Municipal Key Laboratory of Child Development and Nutriomics, Capital Institute of Pediatrics, Beijing, ChinaGraduate School of Peking Union Medical College, Beijing, ChinaIntroductionThis study explores the effects of moderate-intensity exercise on protein lactylation in mouse muscle tissue metabolism.MethodsHealthy adult mice running for 6 weeks as an exercise model and sedentary mice as the control were used to perform transcriptomic, proteomic, lactylation-proteomic, and metabolomic analyses. Correlation analysis between transcriptome and proteome and between proteome and metabolome was also conducted.ResultsIn this study, 159 lactylation sites of 78 proteins were identified as being differentially regulated by moderate-intensity exercise. Enrichment analysis showed that the lactylation of proteins Atp5mg, and Atp5po exhibited ATP hydrolysis activity. Mtatp8 and Atp5po were involved in biological processes such as mitochondrial transmembrane transport, and Mtatp8, Atp5mg, and Atp5po participate in oxidative phosphorylation and thermogenesis pathways. The lactylation levels of Mtatp8, Atp5mg, and Atp5po proteins in the exercise group were significantly decreased, while their protein levels were significantly increased. The combined analysis of proteomics and metabolomics showed that the oxocarboxylic acid metabolism and sphingolipid signaling pathways had significant changes under the influence of moderate-intensity exercise.DiscussionOur results indicate that moderate-intensity exercise has an effect on the lactylation level of mice, possibly by reducing the lactylation levels of Mtatp8, Atp5mg, and Atp5po and increasing the expression of their protein levels, thereby regulating the oxidative phosphorylation pathway and participating in energy metabolism. Further exploration is needed into the 2-oxocarboxylic acid metabolism pathway and the sphingolipid signaling pathway.https://www.frontiersin.org/articles/10.3389/fcell.2024.1472338/fullmoderate-intensity exerciselactylationproteomemetabolomemuscle tissue |
| spellingShingle | Jiahui Chang Jiahui Chang Jiahui Chang Wanyu Wu Wanyu Wu Wanyu Wu Ping Qian Zhaoxu Lu Xuejia He Fang Wang Ting Zhang Ting Zhang Ting Zhang Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue Frontiers in Cell and Developmental Biology moderate-intensity exercise lactylation proteome metabolome muscle tissue |
| title | Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue |
| title_full | Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue |
| title_fullStr | Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue |
| title_full_unstemmed | Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue |
| title_short | Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue |
| title_sort | multi omics study on the effect of moderate intensity exercise on protein lactylation in mouse muscle tissue |
| topic | moderate-intensity exercise lactylation proteome metabolome muscle tissue |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2024.1472338/full |
| work_keys_str_mv | AT jiahuichang multiomicsstudyontheeffectofmoderateintensityexerciseonproteinlactylationinmousemuscletissue AT jiahuichang multiomicsstudyontheeffectofmoderateintensityexerciseonproteinlactylationinmousemuscletissue AT jiahuichang multiomicsstudyontheeffectofmoderateintensityexerciseonproteinlactylationinmousemuscletissue AT wanyuwu multiomicsstudyontheeffectofmoderateintensityexerciseonproteinlactylationinmousemuscletissue AT wanyuwu multiomicsstudyontheeffectofmoderateintensityexerciseonproteinlactylationinmousemuscletissue AT wanyuwu multiomicsstudyontheeffectofmoderateintensityexerciseonproteinlactylationinmousemuscletissue AT pingqian multiomicsstudyontheeffectofmoderateintensityexerciseonproteinlactylationinmousemuscletissue AT zhaoxulu multiomicsstudyontheeffectofmoderateintensityexerciseonproteinlactylationinmousemuscletissue AT xuejiahe multiomicsstudyontheeffectofmoderateintensityexerciseonproteinlactylationinmousemuscletissue AT fangwang multiomicsstudyontheeffectofmoderateintensityexerciseonproteinlactylationinmousemuscletissue AT tingzhang multiomicsstudyontheeffectofmoderateintensityexerciseonproteinlactylationinmousemuscletissue AT tingzhang multiomicsstudyontheeffectofmoderateintensityexerciseonproteinlactylationinmousemuscletissue AT tingzhang multiomicsstudyontheeffectofmoderateintensityexerciseonproteinlactylationinmousemuscletissue |