Role of intestinal mucin-2 in the effectiveness of the treatment of Helicobacter spp. infection in laboratory mice

Abnormal synthesis of the main intestinal proteo­glycan mucin-2 is typical of ulcerative colitis and Crohn’s disease in humans. Those morphological changes of the mucus layer affect the diversity of the intestinal microflora. Antibiotics may be ineffective or even dangerous to humans or animals defi...

Full description

Saved in:
Bibliographic Details
Main Authors: E. A. Litvinova, M. D. Belyaev, A. V. Prokhortchouk, V. S. Korostina, E. B. Prokhortchouk, E. N. Kozhevnikova
Format: Article
Language:English
Published: Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders 2015-12-01
Series:Вавиловский журнал генетики и селекции
Subjects:
Online Access:https://vavilov.elpub.ru/jour/article/view/441
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832575231951110144
author E. A. Litvinova
M. D. Belyaev
A. V. Prokhortchouk
V. S. Korostina
E. B. Prokhortchouk
E. N. Kozhevnikova
author_facet E. A. Litvinova
M. D. Belyaev
A. V. Prokhortchouk
V. S. Korostina
E. B. Prokhortchouk
E. N. Kozhevnikova
author_sort E. A. Litvinova
collection DOAJ
description Abnormal synthesis of the main intestinal proteo­glycan mucin-2 is typical of ulcerative colitis and Crohn’s disease in humans. Those morphological changes of the mucus layer affect the diversity of the intestinal microflora. Antibiotics may be ineffective or even dangerous to humans or animals deficient for mucin-2 because of the risk of sepsis and chronic inflammation. In this study, we investigated the potential of antibiotics (clarithromycin, amoxicillin, and metronidazole) in elimination of patho­genic infection from Muc2 knockout mice (Muc2–/–). We assayed the population sizes of pathogens (Heli­co­bacter spp.) and symbiotic (E. coli) bacteria in the intestines of animals as a criterion of antibiotic efficacy. The damaging effect of antibacterial treatment on the host body was estimated from their survival rate. Three antibiotics were ineffective in the elimination of Helicobacter spp. from mucin-2-deficient mice. Moreover, the mortality of Muc2 knockout mice during the antibacterial treatment was 60 %. The survival of wild-type mice (C57BL/6J) during the treatment was 100 %. The weight of wild-type mice showed no decrease during the treatment. The Helico­bacter spp. pathogen was fully eradicated from wild-type mice. Thus, therapy of Helicobacter spp. infection in mucin-2 deficient animals is not only poorly efficient but even deadly. The high susceptibility to antibiotics allows Muc2 knockout mice to be used as a test model to evaluate the pharmacological safety of new antibiotics.
format Article
id doaj-art-66d6d1173671436abff1abc37e2486ff
institution Kabale University
issn 2500-3259
language English
publishDate 2015-12-01
publisher Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
record_format Article
series Вавиловский журнал генетики и селекции
spelling doaj-art-66d6d1173671436abff1abc37e2486ff2025-02-01T09:58:02ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592015-12-0119449449810.18699/VJ15.066399Role of intestinal mucin-2 in the effectiveness of the treatment of Helicobacter spp. infection in laboratory miceE. A. Litvinova0M. D. Belyaev1A. V. Prokhortchouk2V. S. Korostina3E. B. Prokhortchouk4E. N. Kozhevnikova5Institute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, RussiaInstitute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, RussiaInstitute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaAbnormal synthesis of the main intestinal proteo­glycan mucin-2 is typical of ulcerative colitis and Crohn’s disease in humans. Those morphological changes of the mucus layer affect the diversity of the intestinal microflora. Antibiotics may be ineffective or even dangerous to humans or animals deficient for mucin-2 because of the risk of sepsis and chronic inflammation. In this study, we investigated the potential of antibiotics (clarithromycin, amoxicillin, and metronidazole) in elimination of patho­genic infection from Muc2 knockout mice (Muc2–/–). We assayed the population sizes of pathogens (Heli­co­bacter spp.) and symbiotic (E. coli) bacteria in the intestines of animals as a criterion of antibiotic efficacy. The damaging effect of antibacterial treatment on the host body was estimated from their survival rate. Three antibiotics were ineffective in the elimination of Helicobacter spp. from mucin-2-deficient mice. Moreover, the mortality of Muc2 knockout mice during the antibacterial treatment was 60 %. The survival of wild-type mice (C57BL/6J) during the treatment was 100 %. The weight of wild-type mice showed no decrease during the treatment. The Helico­bacter spp. pathogen was fully eradicated from wild-type mice. Thus, therapy of Helicobacter spp. infection in mucin-2 deficient animals is not only poorly efficient but even deadly. The high susceptibility to antibiotics allows Muc2 knockout mice to be used as a test model to evaluate the pharmacological safety of new antibiotics.https://vavilov.elpub.ru/jour/article/view/441miceintestinal inflammationmucin-2antibacterial treatment
spellingShingle E. A. Litvinova
M. D. Belyaev
A. V. Prokhortchouk
V. S. Korostina
E. B. Prokhortchouk
E. N. Kozhevnikova
Role of intestinal mucin-2 in the effectiveness of the treatment of Helicobacter spp. infection in laboratory mice
Вавиловский журнал генетики и селекции
mice
intestinal inflammation
mucin-2
antibacterial treatment
title Role of intestinal mucin-2 in the effectiveness of the treatment of Helicobacter spp. infection in laboratory mice
title_full Role of intestinal mucin-2 in the effectiveness of the treatment of Helicobacter spp. infection in laboratory mice
title_fullStr Role of intestinal mucin-2 in the effectiveness of the treatment of Helicobacter spp. infection in laboratory mice
title_full_unstemmed Role of intestinal mucin-2 in the effectiveness of the treatment of Helicobacter spp. infection in laboratory mice
title_short Role of intestinal mucin-2 in the effectiveness of the treatment of Helicobacter spp. infection in laboratory mice
title_sort role of intestinal mucin 2 in the effectiveness of the treatment of helicobacter spp infection in laboratory mice
topic mice
intestinal inflammation
mucin-2
antibacterial treatment
url https://vavilov.elpub.ru/jour/article/view/441
work_keys_str_mv AT ealitvinova roleofintestinalmucin2intheeffectivenessofthetreatmentofhelicobactersppinfectioninlaboratorymice
AT mdbelyaev roleofintestinalmucin2intheeffectivenessofthetreatmentofhelicobactersppinfectioninlaboratorymice
AT avprokhortchouk roleofintestinalmucin2intheeffectivenessofthetreatmentofhelicobactersppinfectioninlaboratorymice
AT vskorostina roleofintestinalmucin2intheeffectivenessofthetreatmentofhelicobactersppinfectioninlaboratorymice
AT ebprokhortchouk roleofintestinalmucin2intheeffectivenessofthetreatmentofhelicobactersppinfectioninlaboratorymice
AT enkozhevnikova roleofintestinalmucin2intheeffectivenessofthetreatmentofhelicobactersppinfectioninlaboratorymice