First report of a Plasmodium malariae SSU rRNA gene variant in Africa associated with reduced amplification by nested PCR

Abstract Background Nested polymerase chain reaction (PCR) targeting the small subunit ribosomal ribonucleic acid (SSU rRNA, 18S rRNA) region is widely used to differentiate Plasmodium species. We identified a variant of the Plasmodium malariae SSU rRNA gene that suggests nested PCR may fail to dete...

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Bibliographic Details
Main Authors: Maki Goto, Kei Yamamoto, Kanako Komaki-Yasuda, Shigeyuki Kano, Norio Ohmagari
Format: Article
Language:English
Published: BMC 2025-08-01
Series:Tropical Medicine and Health
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Online Access:https://doi.org/10.1186/s41182-025-00787-5
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