Probing neuronal activity with genetically encoded calcium and voltage fluorescent indicators
Monitoring neural activity in individual neurons is crucial for understanding neural circuits and brain functions. The emergence of optical imaging technologies has dramatically transformed the field of neuroscience, enabling detailed observation of large-scale neuronal populations with both cellula...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | Neuroscience Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0168010224000762 |
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| author | Masayuki Sakamoto Tatsushi Yokoyama |
| author_facet | Masayuki Sakamoto Tatsushi Yokoyama |
| author_sort | Masayuki Sakamoto |
| collection | DOAJ |
| description | Monitoring neural activity in individual neurons is crucial for understanding neural circuits and brain functions. The emergence of optical imaging technologies has dramatically transformed the field of neuroscience, enabling detailed observation of large-scale neuronal populations with both cellular and subcellular resolution. This transformation will be further accelerated by the integration of these imaging technologies and advanced big data analysis. Genetically encoded fluorescent indicators to detect neural activity with high signal-to-noise ratios are pivotal in this advancement. In recent years, these indicators have undergone significant developments, greatly enhancing the understanding of neural dynamics and networks. This review highlights the recent progress in genetically encoded calcium and voltage indicators and discusses the future direction of imaging techniques with big data analysis that deepens our understanding of the complexities of the brain. |
| format | Article |
| id | doaj-art-66ca5d7d005b4bbf8bcee56870c8494d |
| institution | Kabale University |
| issn | 0168-0102 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neuroscience Research |
| spelling | doaj-art-66ca5d7d005b4bbf8bcee56870c8494d2025-08-20T03:53:52ZengElsevierNeuroscience Research0168-01022025-06-01215566310.1016/j.neures.2024.06.004Probing neuronal activity with genetically encoded calcium and voltage fluorescent indicatorsMasayuki Sakamoto0Tatsushi Yokoyama1Corresponding author.; Graduate School of Biostudies, Kyoto University, 53 Shogoin Kawara-cho, Sakyo-ku, Kyoto 606-8507, JapanGraduate School of Biostudies, Kyoto University, 53 Shogoin Kawara-cho, Sakyo-ku, Kyoto 606-8507, JapanMonitoring neural activity in individual neurons is crucial for understanding neural circuits and brain functions. The emergence of optical imaging technologies has dramatically transformed the field of neuroscience, enabling detailed observation of large-scale neuronal populations with both cellular and subcellular resolution. This transformation will be further accelerated by the integration of these imaging technologies and advanced big data analysis. Genetically encoded fluorescent indicators to detect neural activity with high signal-to-noise ratios are pivotal in this advancement. In recent years, these indicators have undergone significant developments, greatly enhancing the understanding of neural dynamics and networks. This review highlights the recent progress in genetically encoded calcium and voltage indicators and discusses the future direction of imaging techniques with big data analysis that deepens our understanding of the complexities of the brain.http://www.sciencedirect.com/science/article/pii/S0168010224000762Calcium imagingVoltage imagingIn vivo imagingFluorescent proteinProtein engineering |
| spellingShingle | Masayuki Sakamoto Tatsushi Yokoyama Probing neuronal activity with genetically encoded calcium and voltage fluorescent indicators Neuroscience Research Calcium imaging Voltage imaging In vivo imaging Fluorescent protein Protein engineering |
| title | Probing neuronal activity with genetically encoded calcium and voltage fluorescent indicators |
| title_full | Probing neuronal activity with genetically encoded calcium and voltage fluorescent indicators |
| title_fullStr | Probing neuronal activity with genetically encoded calcium and voltage fluorescent indicators |
| title_full_unstemmed | Probing neuronal activity with genetically encoded calcium and voltage fluorescent indicators |
| title_short | Probing neuronal activity with genetically encoded calcium and voltage fluorescent indicators |
| title_sort | probing neuronal activity with genetically encoded calcium and voltage fluorescent indicators |
| topic | Calcium imaging Voltage imaging In vivo imaging Fluorescent protein Protein engineering |
| url | http://www.sciencedirect.com/science/article/pii/S0168010224000762 |
| work_keys_str_mv | AT masayukisakamoto probingneuronalactivitywithgeneticallyencodedcalciumandvoltagefluorescentindicators AT tatsushiyokoyama probingneuronalactivitywithgeneticallyencodedcalciumandvoltagefluorescentindicators |