Multi-functionalized probiotics through layer-by-layer coating with tannic acid-Mg2+ and casein phosphopeptide complexes for preventing ulcerative colitis
Gut microbiota imbalance-induced inflammatory response and oxidative stress are two of the main reasons causing ulcerative colitis (UC). Probiotics show potent modulating effects on microbiota imbalance and have been considered as an optimal substitute of antibiotics for preventing UC. However, the...
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Elsevier
2025-04-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006425001796 |
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| author | Zhanyin Qian Zihan Zhai Mingjin Ren Yuanyuan Cheng Mingxin Cao Yue Wang Linyi Dong Chunyu Li Hailong Cao Yinsong Wang |
| author_facet | Zhanyin Qian Zihan Zhai Mingjin Ren Yuanyuan Cheng Mingxin Cao Yue Wang Linyi Dong Chunyu Li Hailong Cao Yinsong Wang |
| author_sort | Zhanyin Qian |
| collection | DOAJ |
| description | Gut microbiota imbalance-induced inflammatory response and oxidative stress are two of the main reasons causing ulcerative colitis (UC). Probiotics show potent modulating effects on microbiota imbalance and have been considered as an optimal substitute of antibiotics for preventing UC. However, the harsh environment of the gastrointestinal tract is not conducive to the survival and persistence of probiotics. Herein, we developed an efficient surface coating strategy to overcome the delivery challenges of probiotics and also endow them with multiple functions through layer-by-layer coating with tannic acid (TA)-Mg2+ and casein phosphopeptide (CPP) complexes. Saccharomyces boulardii (SB), one of yeasts that have been widely applied in the food and pharmaceutical field, was used as a model probiotic for assessing the synergistic effects of this coating strategy on preventing UC. Multi-functionalized probiotic thus prepared (called SB@TA-Mg2+@CPP) had significantly enhanced stability under the simulated gastric and intestinal fluid conditions, and also displayed vigorous cell viability and potent antioxidant activity. In the mouse model of dextran sulfate sodium (DSS)-induced colitis, SB@TA-Mg2+@CPP exhibited strong antioxidant and anti-inflammatory effects, remarkably increased the abundance and diversity of gut microbiota, and maintained gut barrier integrity. Meanwhile, SB@TA-Mg2+@CPP notably improved the adsorption of Mg2+, which also contributed to enhance the preventive effect against DSS-induced colitis. In summary, this study provides an efficient coating strategy to develop multi-functionalized probiotics for preventing UC. |
| format | Article |
| id | doaj-art-66c224a2250147c49d0c8698af79c105 |
| institution | DOAJ |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-66c224a2250147c49d0c8698af79c1052025-08-20T02:47:49ZengElsevierMaterials Today Bio2590-00642025-04-013110162110.1016/j.mtbio.2025.101621Multi-functionalized probiotics through layer-by-layer coating with tannic acid-Mg2+ and casein phosphopeptide complexes for preventing ulcerative colitisZhanyin Qian0Zihan Zhai1Mingjin Ren2Yuanyuan Cheng3Mingxin Cao4Yue Wang5Linyi Dong6Chunyu Li7Hailong Cao8Yinsong Wang9The Province and Ministry Co-Sponsored Collaborative Innovation Center for Medical Epigenetics, Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin, 300070, ChinaDepartment of Gastroenterology and Hepatology, Tianjin Medical University General Hospital, Tianjin Institute of Digestive Diseases, Tianjin Key Laboratory of Digestive Diseases, Tianjin, 300052, ChinaThe Province and Ministry Co-Sponsored Collaborative Innovation Center for Medical Epigenetics, Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin, 300070, ChinaThe Province and Ministry Co-Sponsored Collaborative Innovation Center for Medical Epigenetics, Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin, 300070, ChinaTianjin Key Laboratory of Oral Soft and Hard Tissues Restoration and Regeneration, School and Hospital of Stomatology, Tianjin Medical University, Tianjin, 300070, ChinaTianjin Key Laboratory of Oral Soft and Hard Tissues Restoration and Regeneration, School and Hospital of Stomatology, Tianjin Medical University, Tianjin, 300070, ChinaThe Province and Ministry Co-Sponsored Collaborative Innovation Center for Medical Epigenetics, Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin, 300070, ChinaDepartment of Integrated Traditional Chinese and Western Medicine, International Medical School, Tianjin Medical University, Tianjin, 300070, China; Corresponding author.Department of Gastroenterology and Hepatology, Tianjin Medical University General Hospital, Tianjin Institute of Digestive Diseases, Tianjin Key Laboratory of Digestive Diseases, Tianjin, 300052, China; Corresponding author.The Province and Ministry Co-Sponsored Collaborative Innovation Center for Medical Epigenetics, Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin, 300070, China; Tianjin Key Laboratory of Oral Soft and Hard Tissues Restoration and Regeneration, School and Hospital of Stomatology, Tianjin Medical University, Tianjin, 300070, China; Corresponding author. The Province and Ministry Co-Sponsored Collaborative Innovation Center for Medical Epigenetics, Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin, 300070, China.Gut microbiota imbalance-induced inflammatory response and oxidative stress are two of the main reasons causing ulcerative colitis (UC). Probiotics show potent modulating effects on microbiota imbalance and have been considered as an optimal substitute of antibiotics for preventing UC. However, the harsh environment of the gastrointestinal tract is not conducive to the survival and persistence of probiotics. Herein, we developed an efficient surface coating strategy to overcome the delivery challenges of probiotics and also endow them with multiple functions through layer-by-layer coating with tannic acid (TA)-Mg2+ and casein phosphopeptide (CPP) complexes. Saccharomyces boulardii (SB), one of yeasts that have been widely applied in the food and pharmaceutical field, was used as a model probiotic for assessing the synergistic effects of this coating strategy on preventing UC. Multi-functionalized probiotic thus prepared (called SB@TA-Mg2+@CPP) had significantly enhanced stability under the simulated gastric and intestinal fluid conditions, and also displayed vigorous cell viability and potent antioxidant activity. In the mouse model of dextran sulfate sodium (DSS)-induced colitis, SB@TA-Mg2+@CPP exhibited strong antioxidant and anti-inflammatory effects, remarkably increased the abundance and diversity of gut microbiota, and maintained gut barrier integrity. Meanwhile, SB@TA-Mg2+@CPP notably improved the adsorption of Mg2+, which also contributed to enhance the preventive effect against DSS-induced colitis. In summary, this study provides an efficient coating strategy to develop multi-functionalized probiotics for preventing UC.http://www.sciencedirect.com/science/article/pii/S2590006425001796ProbioticsTannic acidCasein phosphopeptideSurface coatingUlcerative colitis |
| spellingShingle | Zhanyin Qian Zihan Zhai Mingjin Ren Yuanyuan Cheng Mingxin Cao Yue Wang Linyi Dong Chunyu Li Hailong Cao Yinsong Wang Multi-functionalized probiotics through layer-by-layer coating with tannic acid-Mg2+ and casein phosphopeptide complexes for preventing ulcerative colitis Materials Today Bio Probiotics Tannic acid Casein phosphopeptide Surface coating Ulcerative colitis |
| title | Multi-functionalized probiotics through layer-by-layer coating with tannic acid-Mg2+ and casein phosphopeptide complexes for preventing ulcerative colitis |
| title_full | Multi-functionalized probiotics through layer-by-layer coating with tannic acid-Mg2+ and casein phosphopeptide complexes for preventing ulcerative colitis |
| title_fullStr | Multi-functionalized probiotics through layer-by-layer coating with tannic acid-Mg2+ and casein phosphopeptide complexes for preventing ulcerative colitis |
| title_full_unstemmed | Multi-functionalized probiotics through layer-by-layer coating with tannic acid-Mg2+ and casein phosphopeptide complexes for preventing ulcerative colitis |
| title_short | Multi-functionalized probiotics through layer-by-layer coating with tannic acid-Mg2+ and casein phosphopeptide complexes for preventing ulcerative colitis |
| title_sort | multi functionalized probiotics through layer by layer coating with tannic acid mg2 and casein phosphopeptide complexes for preventing ulcerative colitis |
| topic | Probiotics Tannic acid Casein phosphopeptide Surface coating Ulcerative colitis |
| url | http://www.sciencedirect.com/science/article/pii/S2590006425001796 |
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