Microbial diversity and metabolomics analysis of colon contents exposed to cadmium and polystyrene microplastics
Cadmium and microplastics, common pollutants, can accumulate in the body, impacting the intestinal barrier and harming livestock breeding. In order to explore the damage mechanism of cadmium and cadmium combined microplastic on the colon of mice, 60 mice were divided into three groups: The control g...
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324016610 |
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| author | Dechun Chen Liyu Pan Xuan Ran Junyu Huang Xiaohua Teng Falong Yang Haifeng Liu |
| author_facet | Dechun Chen Liyu Pan Xuan Ran Junyu Huang Xiaohua Teng Falong Yang Haifeng Liu |
| author_sort | Dechun Chen |
| collection | DOAJ |
| description | Cadmium and microplastics, common pollutants, can accumulate in the body, impacting the intestinal barrier and harming livestock breeding. In order to explore the damage mechanism of cadmium and cadmium combined microplastic on the colon of mice, 60 mice were divided into three groups: The control group (0.2 mL of saline), cadmium group (Cd group, 0.2 mL of 4.8 mg/kg/d CdCl2) and mixed group (Mix group, 0.2 mL of mixed solution containing 4.8 mg/kg/d CdCl2 and 10.0 mg/d MPs) were fed for 42 d. The changes of colon histopathology were observed, and the changes of microbial diversity and metabolomics of colon contents were analyzed. Pathological sections of the colon showed abnormal mucosal hyperemia with mixed exposure compared to cadmium exposure. Microbial diversity analysis showed increased abundances of Enterococcus, Adlercreutzia, and Bifidobacterium in the Cd and Mix groups, with Dubosiella being the most significantly increased. Metabolomic analysis indicated significant differences in nucleotide and purine metabolism between the Cd and control groups, and in linoleic acid and bile acid metabolism between the Mix and control groups. The ABC transporter metabolites increased with Cd exposure, while the PPAR pathway metabolites were enriched with MPs exposure. Correlation analysis highlighted several key findings: Pasteurella exhibited a notably negative association with pantothenate. Conversely, Enterococcus demonstrated a significant positive link with palmitoylcarnitine. Additionally, both Adlercreutzia and norank_f_Eggerthellaceae showed a positive correlation with azelaic acid. These findings suggest that Cd and MPs disrupt intestinal microbiota and metabolic pathways, providing insights into potential treatments for such exposures. |
| format | Article |
| id | doaj-art-66bdf3059cd84732b15df0ca52b68923 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-66bdf3059cd84732b15df0ca52b689232025-02-12T05:29:54ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01290117585Microbial diversity and metabolomics analysis of colon contents exposed to cadmium and polystyrene microplasticsDechun Chen0Liyu Pan1Xuan Ran2Junyu Huang3Xiaohua Teng4Falong Yang5Haifeng Liu6Key Laboratory of Animal Medicine of Sichuan Province, Southwest Minzu University, Chengdu 610041, ChinaKey Laboratory of Animal Medicine of Sichuan Province, Southwest Minzu University, Chengdu 610041, ChinaKey Laboratory of Animal Medicine of Sichuan Province, Southwest Minzu University, Chengdu 610041, ChinaKey Laboratory of Animal Medicine of Sichuan Province, Southwest Minzu University, Chengdu 610041, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Animal Medicine of Sichuan Province, Southwest Minzu University, Chengdu 610041, China; Correspondence to: College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China.Department of Veterinary Surgery, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China; Corresponding author.Cadmium and microplastics, common pollutants, can accumulate in the body, impacting the intestinal barrier and harming livestock breeding. In order to explore the damage mechanism of cadmium and cadmium combined microplastic on the colon of mice, 60 mice were divided into three groups: The control group (0.2 mL of saline), cadmium group (Cd group, 0.2 mL of 4.8 mg/kg/d CdCl2) and mixed group (Mix group, 0.2 mL of mixed solution containing 4.8 mg/kg/d CdCl2 and 10.0 mg/d MPs) were fed for 42 d. The changes of colon histopathology were observed, and the changes of microbial diversity and metabolomics of colon contents were analyzed. Pathological sections of the colon showed abnormal mucosal hyperemia with mixed exposure compared to cadmium exposure. Microbial diversity analysis showed increased abundances of Enterococcus, Adlercreutzia, and Bifidobacterium in the Cd and Mix groups, with Dubosiella being the most significantly increased. Metabolomic analysis indicated significant differences in nucleotide and purine metabolism between the Cd and control groups, and in linoleic acid and bile acid metabolism between the Mix and control groups. The ABC transporter metabolites increased with Cd exposure, while the PPAR pathway metabolites were enriched with MPs exposure. Correlation analysis highlighted several key findings: Pasteurella exhibited a notably negative association with pantothenate. Conversely, Enterococcus demonstrated a significant positive link with palmitoylcarnitine. Additionally, both Adlercreutzia and norank_f_Eggerthellaceae showed a positive correlation with azelaic acid. These findings suggest that Cd and MPs disrupt intestinal microbiota and metabolic pathways, providing insights into potential treatments for such exposures.http://www.sciencedirect.com/science/article/pii/S0147651324016610CadmiumMicroplasticsIntestinal barrierMicrobial diversityMetabolomics |
| spellingShingle | Dechun Chen Liyu Pan Xuan Ran Junyu Huang Xiaohua Teng Falong Yang Haifeng Liu Microbial diversity and metabolomics analysis of colon contents exposed to cadmium and polystyrene microplastics Ecotoxicology and Environmental Safety Cadmium Microplastics Intestinal barrier Microbial diversity Metabolomics |
| title | Microbial diversity and metabolomics analysis of colon contents exposed to cadmium and polystyrene microplastics |
| title_full | Microbial diversity and metabolomics analysis of colon contents exposed to cadmium and polystyrene microplastics |
| title_fullStr | Microbial diversity and metabolomics analysis of colon contents exposed to cadmium and polystyrene microplastics |
| title_full_unstemmed | Microbial diversity and metabolomics analysis of colon contents exposed to cadmium and polystyrene microplastics |
| title_short | Microbial diversity and metabolomics analysis of colon contents exposed to cadmium and polystyrene microplastics |
| title_sort | microbial diversity and metabolomics analysis of colon contents exposed to cadmium and polystyrene microplastics |
| topic | Cadmium Microplastics Intestinal barrier Microbial diversity Metabolomics |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324016610 |
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