The mRNA expression levels of calpains and their activity in malignant and dysplastic epithelium of the upper respiratory tract
Background. The calpain proteolytic system plays an important role in the development of cancer. Detection of early cancer in the upper respiratory tract is often challenging, as symptoms are largely non-specific, and most cases are diagnosed at an advanced stage.Aim. To identify candidate markers o...
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Siberian State Medical University (Tomsk)
2021-07-01
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| Series: | Бюллетень сибирской медицины |
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| Online Access: | https://bulletin.ssmu.ru/jour/article/view/4383 |
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| author | E. A. Sidenko G. V. Kakurina O. V. Cheremisina L. V. Spirina E. E. Shashova D. A. Korshunov I. V. Kondakova |
| author_facet | E. A. Sidenko G. V. Kakurina O. V. Cheremisina L. V. Spirina E. E. Shashova D. A. Korshunov I. V. Kondakova |
| author_sort | E. A. Sidenko |
| collection | DOAJ |
| description | Background. The calpain proteolytic system plays an important role in the development of cancer. Detection of early cancer in the upper respiratory tract is often challenging, as symptoms are largely non-specific, and most cases are diagnosed at an advanced stage.Aim. To identify candidate markers of transition from premalignant lesions to invasive carcinoma, we studied mRNA expression levels of CAPN1 and CAPN2 and the total activity of calpains in the tumor tissues of patients with head and neck squamous cell carcinoma (HNSCC) and in the epithelial dysplasia-affected tissues of patients with chronic diseases of the upper respiratory tract.Materials and methods. The study included 32 patients with HNSCC (Т1-3N0-1М0) and 12 patients with chronic diseases of the upper respiratory system associated with epithelial dysplasia. The expression levels of CAPN1 and CAPN2 were assessed using real-time polymerase chain reaction (PCR). The calpain activity was determined by hydrolysis of the fluorogenic Suc-LLVY-AMC oligopeptide.Results. The mRNA expression levels of CAPN1 and CAPN2 were respectively 3 and 4 times higher in the tumor tissue of patients with HNSCC than in the tissue of patients with endothelial dysplasia in the upper respiratory tract. The level of calpain activity was 4.4 times higher in patients with HNSCC than in patients with epithelial dysplasia of different severity.Conclusion. The elevated mRNA expression levels of CAPN1 and CAPN2 and their activity in the tumor tissues of patients with HNSCC compared to patients with chronic respiratory diseases associated with epithelial dysplasia are likely to characterize a high potential for transition from precancerous lesion to cancer. To clarify the role of calpains in the carcinogenesis of HNSCC, further studies of intact tissues using animal models are required. |
| format | Article |
| id | doaj-art-66bdbf93deb74cd8b7faeec0e8b455e6 |
| institution | Kabale University |
| issn | 1682-0363 1819-3684 |
| language | English |
| publishDate | 2021-07-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj-art-66bdbf93deb74cd8b7faeec0e8b455e62025-08-20T03:38:06ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842021-07-01202889410.20538/1682-0363-2021-2-88-942785The mRNA expression levels of calpains and their activity in malignant and dysplastic epithelium of the upper respiratory tractE. A. Sidenko0G. V. Kakurina1O. V. Cheremisina2L. V. Spirina3E. E. Shashova4D. A. Korshunov5I. V. Kondakova6Cancer Research Institute, Tomsk National Research Medical Center (NRMC) Russian Academy of SciencesCancer Research Institute, Tomsk National Research Medical Center (NRMC) Russian Academy of SciencesCancer Research Institute, Tomsk National Research Medical Center (NRMC) Russian Academy of SciencesCancer Research Institute, Tomsk National Research Medical Center (NRMC) Russian Academy of SciencesCancer Research Institute, Tomsk National Research Medical Center (NRMC) Russian Academy of SciencesCancer Research Institute, Tomsk National Research Medical Center (NRMC) Russian Academy of SciencesCancer Research Institute, Tomsk National Research Medical Center (NRMC) Russian Academy of SciencesBackground. The calpain proteolytic system plays an important role in the development of cancer. Detection of early cancer in the upper respiratory tract is often challenging, as symptoms are largely non-specific, and most cases are diagnosed at an advanced stage.Aim. To identify candidate markers of transition from premalignant lesions to invasive carcinoma, we studied mRNA expression levels of CAPN1 and CAPN2 and the total activity of calpains in the tumor tissues of patients with head and neck squamous cell carcinoma (HNSCC) and in the epithelial dysplasia-affected tissues of patients with chronic diseases of the upper respiratory tract.Materials and methods. The study included 32 patients with HNSCC (Т1-3N0-1М0) and 12 patients with chronic diseases of the upper respiratory system associated with epithelial dysplasia. The expression levels of CAPN1 and CAPN2 were assessed using real-time polymerase chain reaction (PCR). The calpain activity was determined by hydrolysis of the fluorogenic Suc-LLVY-AMC oligopeptide.Results. The mRNA expression levels of CAPN1 and CAPN2 were respectively 3 and 4 times higher in the tumor tissue of patients with HNSCC than in the tissue of patients with endothelial dysplasia in the upper respiratory tract. The level of calpain activity was 4.4 times higher in patients with HNSCC than in patients with epithelial dysplasia of different severity.Conclusion. The elevated mRNA expression levels of CAPN1 and CAPN2 and their activity in the tumor tissues of patients with HNSCC compared to patients with chronic respiratory diseases associated with epithelial dysplasia are likely to characterize a high potential for transition from precancerous lesion to cancer. To clarify the role of calpains in the carcinogenesis of HNSCC, further studies of intact tissues using animal models are required.https://bulletin.ssmu.ru/jour/article/view/4383head and neck squamous cell carcinomaepithelial dysplasiacalpain activitymrna expression of capn1 and capn2 |
| spellingShingle | E. A. Sidenko G. V. Kakurina O. V. Cheremisina L. V. Spirina E. E. Shashova D. A. Korshunov I. V. Kondakova The mRNA expression levels of calpains and their activity in malignant and dysplastic epithelium of the upper respiratory tract Бюллетень сибирской медицины head and neck squamous cell carcinoma epithelial dysplasia calpain activity mrna expression of capn1 and capn2 |
| title | The mRNA expression levels of calpains and their activity in malignant and dysplastic epithelium of the upper respiratory tract |
| title_full | The mRNA expression levels of calpains and their activity in malignant and dysplastic epithelium of the upper respiratory tract |
| title_fullStr | The mRNA expression levels of calpains and their activity in malignant and dysplastic epithelium of the upper respiratory tract |
| title_full_unstemmed | The mRNA expression levels of calpains and their activity in malignant and dysplastic epithelium of the upper respiratory tract |
| title_short | The mRNA expression levels of calpains and their activity in malignant and dysplastic epithelium of the upper respiratory tract |
| title_sort | mrna expression levels of calpains and their activity in malignant and dysplastic epithelium of the upper respiratory tract |
| topic | head and neck squamous cell carcinoma epithelial dysplasia calpain activity mrna expression of capn1 and capn2 |
| url | https://bulletin.ssmu.ru/jour/article/view/4383 |
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