UPLC-Q-TOF/MS-based metabolomics reveals modulatory effects of Mesona chinensis Benth polysaccharide in liver injury mice induced by cyclophosphamide
The main purpose of this study was to investigate the improvement effect of Mesona chinensis Benth polysaccharide (MP) on cyclophosphamide (CTX) induced liver injury in mice. To explore metabolic profile of liver tissue and feces among normal group, CTX-induced group and MP management group based on...
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Tsinghua University Press
2023-03-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022001732 |
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| author | Yuzhen Hong Mingyue Shen Qiang Yu Yi Chen Jianhua Xie |
| author_facet | Yuzhen Hong Mingyue Shen Qiang Yu Yi Chen Jianhua Xie |
| author_sort | Yuzhen Hong |
| collection | DOAJ |
| description | The main purpose of this study was to investigate the improvement effect of Mesona chinensis Benth polysaccharide (MP) on cyclophosphamide (CTX) induced liver injury in mice. To explore metabolic profile of liver tissue and feces among normal group, CTX-induced group and MP management group based on metabolomics method by using UPLC-Q-TOF/MS. The results showed that MP could alleviate liver injury and promote the production of short chain fatty acids (SCFAs), with the best dose of 200 mg/kg·body weight (bw). The principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) scores plots of the liver and feces samples showed a clear separation among normal, model and high-dose of MP (MPH). There were 18 endogenous metabolites in liver and 29 endogenous metabolites in feces, which were mainly involved in 8 metabolic pathways: taurine and hypotaurine metabolism, phenylalanine metabolism, α-linolenic acid metabolism, tricarboxylic acid (TCA) cycle, phenylalanine, tyrosine and tryptophan biosynthesis, arachidonic acid metabolism, sphingolipid metabolism as well as tryptophan metabolism. Moreover, a common metabolite arachidonic acid was observed in liver and feces samples. These endogenous metabolites may be considered to be MP's response to liver protection. It will help to further understand the mechanism of MP and provide a basis for further research. |
| format | Article |
| id | doaj-art-66bc964add364a799803f0d040a2baa6 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-66bc964add364a799803f0d040a2baa62025-02-02T23:24:43ZengTsinghua University PressFood Science and Human Wellness2213-45302023-03-01122584595UPLC-Q-TOF/MS-based metabolomics reveals modulatory effects of Mesona chinensis Benth polysaccharide in liver injury mice induced by cyclophosphamideYuzhen Hong0Mingyue Shen1Qiang Yu2Yi Chen3Jianhua Xie4State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaCorresponding author at: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaThe main purpose of this study was to investigate the improvement effect of Mesona chinensis Benth polysaccharide (MP) on cyclophosphamide (CTX) induced liver injury in mice. To explore metabolic profile of liver tissue and feces among normal group, CTX-induced group and MP management group based on metabolomics method by using UPLC-Q-TOF/MS. The results showed that MP could alleviate liver injury and promote the production of short chain fatty acids (SCFAs), with the best dose of 200 mg/kg·body weight (bw). The principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) scores plots of the liver and feces samples showed a clear separation among normal, model and high-dose of MP (MPH). There were 18 endogenous metabolites in liver and 29 endogenous metabolites in feces, which were mainly involved in 8 metabolic pathways: taurine and hypotaurine metabolism, phenylalanine metabolism, α-linolenic acid metabolism, tricarboxylic acid (TCA) cycle, phenylalanine, tyrosine and tryptophan biosynthesis, arachidonic acid metabolism, sphingolipid metabolism as well as tryptophan metabolism. Moreover, a common metabolite arachidonic acid was observed in liver and feces samples. These endogenous metabolites may be considered to be MP's response to liver protection. It will help to further understand the mechanism of MP and provide a basis for further research.http://www.sciencedirect.com/science/article/pii/S2213453022001732MetabolomicsPolysaccharideLiver injuryCyclophosphamide |
| spellingShingle | Yuzhen Hong Mingyue Shen Qiang Yu Yi Chen Jianhua Xie UPLC-Q-TOF/MS-based metabolomics reveals modulatory effects of Mesona chinensis Benth polysaccharide in liver injury mice induced by cyclophosphamide Food Science and Human Wellness Metabolomics Polysaccharide Liver injury Cyclophosphamide |
| title | UPLC-Q-TOF/MS-based metabolomics reveals modulatory effects of Mesona chinensis Benth polysaccharide in liver injury mice induced by cyclophosphamide |
| title_full | UPLC-Q-TOF/MS-based metabolomics reveals modulatory effects of Mesona chinensis Benth polysaccharide in liver injury mice induced by cyclophosphamide |
| title_fullStr | UPLC-Q-TOF/MS-based metabolomics reveals modulatory effects of Mesona chinensis Benth polysaccharide in liver injury mice induced by cyclophosphamide |
| title_full_unstemmed | UPLC-Q-TOF/MS-based metabolomics reveals modulatory effects of Mesona chinensis Benth polysaccharide in liver injury mice induced by cyclophosphamide |
| title_short | UPLC-Q-TOF/MS-based metabolomics reveals modulatory effects of Mesona chinensis Benth polysaccharide in liver injury mice induced by cyclophosphamide |
| title_sort | uplc q tof ms based metabolomics reveals modulatory effects of mesona chinensis benth polysaccharide in liver injury mice induced by cyclophosphamide |
| topic | Metabolomics Polysaccharide Liver injury Cyclophosphamide |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022001732 |
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