Somatic cytogenotoxicity of first-line anti-tuberculosis drugs using the mouse micronucleus assay
Rifampicin, isoniazid, ethambutol, pyrazinamide, and/or their fixed-dose combination (FDC) are the first-line tuberculosis (TB) antibiotics used as a 6-month regimen. We evaluated the cytogenotoxic effects of these drugs using the murine micronucleus assay. Male Swiss albino mice were int...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Academia.edu Journals
2025-01-01
|
| Series: | Academia Biology |
| Online Access: | https://www.academia.edu/127362846/Somatic_cytogenotoxicity_of_first_line_anti_tuberculosis_drugs_using_the_mouse_micronucleus_assay |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850211879000997888 |
|---|---|
| author | Adekunle A. Bakare Charles T. Beckley Obi M. Ebelechukwu Adeyinka M. Gbadebo Motunrayo M. Coker Omonike C. Bakare Olukunle S. Fagbenro Okunola A. Alabi |
| author_facet | Adekunle A. Bakare Charles T. Beckley Obi M. Ebelechukwu Adeyinka M. Gbadebo Motunrayo M. Coker Omonike C. Bakare Olukunle S. Fagbenro Okunola A. Alabi |
| author_sort | Adekunle A. Bakare |
| collection | DOAJ |
| description |
Rifampicin, isoniazid, ethambutol, pyrazinamide, and/or their fixed-dose combination (FDC) are the first-line tuberculosis (TB) antibiotics used as a 6-month regimen. We evaluated the cytogenotoxic effects of these drugs using the murine micronucleus assay. Male Swiss albino mice were intraperitoneally exposed for 5 consecutive days to 1.25, 2.50, 5.00, 10.00, and 20.00 mg/kg bw of rifampicin; 0.63, 1.25, 2.50, 5.00, and 10.00 mg/kg bw of isoniazid; 1.88, 3.75, 7.50, 15.00, and 30.00 mg/kg bw of ethambutol; 3.13, 6.25, 12.50, 25.00, and 50.00 mg/kg bw of pyrazinamide; and 3.50, 7.00, 14.00, 28.00, and 56.00 mg/kg bw of FDC. Normal saline and cyclophosphamide (20 mg/kg bw) were used as negative and positive controls, respectively. At the tested doses, the drugs induced a dose-independent increase in micronucleated polychromatic erythrocyte (MNPCE) and micronucleated normochromatic erythrocyte (MNNCE) compared with the negative control. The induction was statistically significant at most of the tested doses of the drugs. There was a significant increase in the percentage PCE:NCE ratio in the bone marrow cells of mice at the tested doses of isoniazid, pyrazinamide, and the FDC. These results indicate that the individual anti-TB drug and their FDC possess DNA-damaging potential in the somatic cells of mice. |
| format | Article |
| id | doaj-art-66a8e427089a4f96a72d2749941cdda5 |
| institution | OA Journals |
| issn | 2837-4010 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Academia.edu Journals |
| record_format | Article |
| series | Academia Biology |
| spelling | doaj-art-66a8e427089a4f96a72d2749941cdda52025-08-20T02:09:28ZengAcademia.edu JournalsAcademia Biology2837-40102025-01-013110.20935/AcadBiol7503Somatic cytogenotoxicity of first-line anti-tuberculosis drugs using the mouse micronucleus assayAdekunle A. Bakare0Charles T. Beckley1Obi M. Ebelechukwu2Adeyinka M. Gbadebo3Motunrayo M. Coker4Omonike C. Bakare5Olukunle S. Fagbenro6Okunola A. Alabi7Department of Zoology, Cell Biology and Genetics Unit, University of Ibadan, Ibadan, Oyo State 200005, Nigeria.Department of Zoology, Cell Biology and Genetics Unit, University of Ibadan, Ibadan, Oyo State 200005, Nigeria.Department of Zoology, Cell Biology and Genetics Unit, University of Ibadan, Ibadan, Oyo State 200005, Nigeria.Department of Molecular Biology and Biotechnology, Chrisland University, Owode, Abeokuta, Ogun State 23409, Nigeria.Department of Zoology, Cell Biology and Genetics Unit, University of Ibadan, Ibadan, Oyo State 200005, Nigeria.Department of Biological Sciences, Lead City University, Ibadan, Oyo State 200255, Nigeria.Department of Zoology, Cell Biology and Genetics Unit, University of Ibadan, Ibadan, Oyo State 200005, Nigeria.Department of Biology, Federal University of Technology, Akure, Ondo State 340001, Nigeria. Rifampicin, isoniazid, ethambutol, pyrazinamide, and/or their fixed-dose combination (FDC) are the first-line tuberculosis (TB) antibiotics used as a 6-month regimen. We evaluated the cytogenotoxic effects of these drugs using the murine micronucleus assay. Male Swiss albino mice were intraperitoneally exposed for 5 consecutive days to 1.25, 2.50, 5.00, 10.00, and 20.00 mg/kg bw of rifampicin; 0.63, 1.25, 2.50, 5.00, and 10.00 mg/kg bw of isoniazid; 1.88, 3.75, 7.50, 15.00, and 30.00 mg/kg bw of ethambutol; 3.13, 6.25, 12.50, 25.00, and 50.00 mg/kg bw of pyrazinamide; and 3.50, 7.00, 14.00, 28.00, and 56.00 mg/kg bw of FDC. Normal saline and cyclophosphamide (20 mg/kg bw) were used as negative and positive controls, respectively. At the tested doses, the drugs induced a dose-independent increase in micronucleated polychromatic erythrocyte (MNPCE) and micronucleated normochromatic erythrocyte (MNNCE) compared with the negative control. The induction was statistically significant at most of the tested doses of the drugs. There was a significant increase in the percentage PCE:NCE ratio in the bone marrow cells of mice at the tested doses of isoniazid, pyrazinamide, and the FDC. These results indicate that the individual anti-TB drug and their FDC possess DNA-damaging potential in the somatic cells of mice.https://www.academia.edu/127362846/Somatic_cytogenotoxicity_of_first_line_anti_tuberculosis_drugs_using_the_mouse_micronucleus_assay |
| spellingShingle | Adekunle A. Bakare Charles T. Beckley Obi M. Ebelechukwu Adeyinka M. Gbadebo Motunrayo M. Coker Omonike C. Bakare Olukunle S. Fagbenro Okunola A. Alabi Somatic cytogenotoxicity of first-line anti-tuberculosis drugs using the mouse micronucleus assay Academia Biology |
| title | Somatic cytogenotoxicity of first-line anti-tuberculosis drugs using the mouse micronucleus assay |
| title_full | Somatic cytogenotoxicity of first-line anti-tuberculosis drugs using the mouse micronucleus assay |
| title_fullStr | Somatic cytogenotoxicity of first-line anti-tuberculosis drugs using the mouse micronucleus assay |
| title_full_unstemmed | Somatic cytogenotoxicity of first-line anti-tuberculosis drugs using the mouse micronucleus assay |
| title_short | Somatic cytogenotoxicity of first-line anti-tuberculosis drugs using the mouse micronucleus assay |
| title_sort | somatic cytogenotoxicity of first line anti tuberculosis drugs using the mouse micronucleus assay |
| url | https://www.academia.edu/127362846/Somatic_cytogenotoxicity_of_first_line_anti_tuberculosis_drugs_using_the_mouse_micronucleus_assay |
| work_keys_str_mv | AT adekunleabakare somaticcytogenotoxicityoffirstlineantituberculosisdrugsusingthemousemicronucleusassay AT charlestbeckley somaticcytogenotoxicityoffirstlineantituberculosisdrugsusingthemousemicronucleusassay AT obimebelechukwu somaticcytogenotoxicityoffirstlineantituberculosisdrugsusingthemousemicronucleusassay AT adeyinkamgbadebo somaticcytogenotoxicityoffirstlineantituberculosisdrugsusingthemousemicronucleusassay AT motunrayomcoker somaticcytogenotoxicityoffirstlineantituberculosisdrugsusingthemousemicronucleusassay AT omonikecbakare somaticcytogenotoxicityoffirstlineantituberculosisdrugsusingthemousemicronucleusassay AT olukunlesfagbenro somaticcytogenotoxicityoffirstlineantituberculosisdrugsusingthemousemicronucleusassay AT okunolaaalabi somaticcytogenotoxicityoffirstlineantituberculosisdrugsusingthemousemicronucleusassay |