Lateral flow immunochromatographic assay for rapid detection of dichlorvos residue in fruits and vegetables
Dichlorvos (DDVP), a widely used yet environmentally hazardous organophosphate pesticide, necessitates rapid field monitoring due to increasing regulatory restrictions. In this study, we firstly present a gold-nanoparticle-based lateral flow immunochromatographic assay (LFIA) employing a highly spec...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-05-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157525004602 |
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| author | Bao-Zhu Jia Yan-Yan Liu Feng-Yan Chen Phannika Tongchai Sumed Yadoung Anurak Wongta Zhen-Lin Xu Lin Luo Surat Hongsibsong |
| author_facet | Bao-Zhu Jia Yan-Yan Liu Feng-Yan Chen Phannika Tongchai Sumed Yadoung Anurak Wongta Zhen-Lin Xu Lin Luo Surat Hongsibsong |
| author_sort | Bao-Zhu Jia |
| collection | DOAJ |
| description | Dichlorvos (DDVP), a widely used yet environmentally hazardous organophosphate pesticide, necessitates rapid field monitoring due to increasing regulatory restrictions. In this study, we firstly present a gold-nanoparticle-based lateral flow immunochromatographic assay (LFIA) employing a highly specific anti-DDVP monoclonal antibody for onsite DDVP detection within 20 min. The optimized LFIA exhibited exceptional analytical performance, with limits of detection spanning 16 μg/kg to 108 μg/kg across six fruit and vegetable matrices—all below the established maximum residue limits in foods. Method validation with spiked fruit and vegetable samples showed recovery rates of 87.3–109.4 % and 89.2–103.3 %, respectively, with coefficients of variation below 8.3 % and 7.9 %, respectively, confirming the accuracy and precision of the assay. Notably, LFIA exhibited good agreement with standard GC–MS/MS analysis when applied to 144 authentic samples, further validating its reliability for real-world applications. This LFIA offers key advantages including portability, cost-effectiveness, and compatibility with regulatory thresholds, making it an ideal alternative to conventional instrumental methods for routine pesticide residue screening in food safety supervision settings. |
| format | Article |
| id | doaj-art-66a8327c85a74e7d85cf25410c65aaeb |
| institution | OA Journals |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-66a8327c85a74e7d85cf25410c65aaeb2025-08-20T02:31:08ZengElsevierFood Chemistry: X2590-15752025-05-012810261310.1016/j.fochx.2025.102613Lateral flow immunochromatographic assay for rapid detection of dichlorvos residue in fruits and vegetablesBao-Zhu Jia0Yan-Yan Liu1Feng-Yan Chen2Phannika Tongchai3Sumed Yadoung4Anurak Wongta5Zhen-Lin Xu6Lin Luo7Surat Hongsibsong8School of Health Sciences Research, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand; College of Biology and Food Engineering, Guangdong University of Education, Guangzhou 510303, ChinaCollege of Biology and Food Engineering, Guangdong University of Education, Guangzhou 510303, ChinaGuangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaSchool of Health Sciences Research, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, ThailandSchool of Health Sciences Research, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, ThailandSchool of Health Sciences Research, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, ThailandGuangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China; Corresponding authors.School of Health Sciences Research, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand; Corresponding authors.Dichlorvos (DDVP), a widely used yet environmentally hazardous organophosphate pesticide, necessitates rapid field monitoring due to increasing regulatory restrictions. In this study, we firstly present a gold-nanoparticle-based lateral flow immunochromatographic assay (LFIA) employing a highly specific anti-DDVP monoclonal antibody for onsite DDVP detection within 20 min. The optimized LFIA exhibited exceptional analytical performance, with limits of detection spanning 16 μg/kg to 108 μg/kg across six fruit and vegetable matrices—all below the established maximum residue limits in foods. Method validation with spiked fruit and vegetable samples showed recovery rates of 87.3–109.4 % and 89.2–103.3 %, respectively, with coefficients of variation below 8.3 % and 7.9 %, respectively, confirming the accuracy and precision of the assay. Notably, LFIA exhibited good agreement with standard GC–MS/MS analysis when applied to 144 authentic samples, further validating its reliability for real-world applications. This LFIA offers key advantages including portability, cost-effectiveness, and compatibility with regulatory thresholds, making it an ideal alternative to conventional instrumental methods for routine pesticide residue screening in food safety supervision settings.http://www.sciencedirect.com/science/article/pii/S2590157525004602DichlorvosLateral flow immunochromatographic assayImmunoassayRapid detectionFood safety |
| spellingShingle | Bao-Zhu Jia Yan-Yan Liu Feng-Yan Chen Phannika Tongchai Sumed Yadoung Anurak Wongta Zhen-Lin Xu Lin Luo Surat Hongsibsong Lateral flow immunochromatographic assay for rapid detection of dichlorvos residue in fruits and vegetables Food Chemistry: X Dichlorvos Lateral flow immunochromatographic assay Immunoassay Rapid detection Food safety |
| title | Lateral flow immunochromatographic assay for rapid detection of dichlorvos residue in fruits and vegetables |
| title_full | Lateral flow immunochromatographic assay for rapid detection of dichlorvos residue in fruits and vegetables |
| title_fullStr | Lateral flow immunochromatographic assay for rapid detection of dichlorvos residue in fruits and vegetables |
| title_full_unstemmed | Lateral flow immunochromatographic assay for rapid detection of dichlorvos residue in fruits and vegetables |
| title_short | Lateral flow immunochromatographic assay for rapid detection of dichlorvos residue in fruits and vegetables |
| title_sort | lateral flow immunochromatographic assay for rapid detection of dichlorvos residue in fruits and vegetables |
| topic | Dichlorvos Lateral flow immunochromatographic assay Immunoassay Rapid detection Food safety |
| url | http://www.sciencedirect.com/science/article/pii/S2590157525004602 |
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