Multielectrode array characterization of human induced pluripotent stem cell derived neurons in co-culture with primary human astrocytes.
Human induced pluripotent stem cells (hiPSCs) derived into neurons offer a powerful in vitro model to study cellular processes. One method to characterize functional network properties of these cells is using multielectrode arrays (MEAs). MEAs can measure the electrophysiological activity of cellula...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2024-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0303901&type=printable |
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| author | Maddie R Lemieux Bernhard Freigassner Jessica L Hanson Zahra Thathey Mark R Opp Charles A Hoeffer Christopher D Link |
| author_facet | Maddie R Lemieux Bernhard Freigassner Jessica L Hanson Zahra Thathey Mark R Opp Charles A Hoeffer Christopher D Link |
| author_sort | Maddie R Lemieux |
| collection | DOAJ |
| description | Human induced pluripotent stem cells (hiPSCs) derived into neurons offer a powerful in vitro model to study cellular processes. One method to characterize functional network properties of these cells is using multielectrode arrays (MEAs). MEAs can measure the electrophysiological activity of cellular cultures for extended periods of time without disruption. Here we used WTC11 hiPSCs with a doxycycline-inducible neurogenin 2 (NGN2) transgene differentiated into neurons co-cultured with primary human astrocytes. We achieved a synchrony index ∼0.9 in as little as six-weeks with a mean firing rate of ∼13 Hz. Previous reports show that derived 3D brain organoids can take several months to achieve similar strong network burst synchrony. We also used this co-culture to model aspects of blood-brain barrier breakdown by using human serum. Our fully human co-culture achieved strong network burst synchrony in a fraction of the time of previous reports, making it an excellent first pass, high-throughput method for studying network properties and neurodegenerative diseases. |
| format | Article |
| id | doaj-art-66a68061811e4ec9ae912fcfbf232230 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-66a68061811e4ec9ae912fcfbf2322302025-08-20T03:51:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-01196e030390110.1371/journal.pone.0303901Multielectrode array characterization of human induced pluripotent stem cell derived neurons in co-culture with primary human astrocytes.Maddie R LemieuxBernhard FreigassnerJessica L HansonZahra ThatheyMark R OppCharles A HoefferChristopher D LinkHuman induced pluripotent stem cells (hiPSCs) derived into neurons offer a powerful in vitro model to study cellular processes. One method to characterize functional network properties of these cells is using multielectrode arrays (MEAs). MEAs can measure the electrophysiological activity of cellular cultures for extended periods of time without disruption. Here we used WTC11 hiPSCs with a doxycycline-inducible neurogenin 2 (NGN2) transgene differentiated into neurons co-cultured with primary human astrocytes. We achieved a synchrony index ∼0.9 in as little as six-weeks with a mean firing rate of ∼13 Hz. Previous reports show that derived 3D brain organoids can take several months to achieve similar strong network burst synchrony. We also used this co-culture to model aspects of blood-brain barrier breakdown by using human serum. Our fully human co-culture achieved strong network burst synchrony in a fraction of the time of previous reports, making it an excellent first pass, high-throughput method for studying network properties and neurodegenerative diseases.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0303901&type=printable |
| spellingShingle | Maddie R Lemieux Bernhard Freigassner Jessica L Hanson Zahra Thathey Mark R Opp Charles A Hoeffer Christopher D Link Multielectrode array characterization of human induced pluripotent stem cell derived neurons in co-culture with primary human astrocytes. PLoS ONE |
| title | Multielectrode array characterization of human induced pluripotent stem cell derived neurons in co-culture with primary human astrocytes. |
| title_full | Multielectrode array characterization of human induced pluripotent stem cell derived neurons in co-culture with primary human astrocytes. |
| title_fullStr | Multielectrode array characterization of human induced pluripotent stem cell derived neurons in co-culture with primary human astrocytes. |
| title_full_unstemmed | Multielectrode array characterization of human induced pluripotent stem cell derived neurons in co-culture with primary human astrocytes. |
| title_short | Multielectrode array characterization of human induced pluripotent stem cell derived neurons in co-culture with primary human astrocytes. |
| title_sort | multielectrode array characterization of human induced pluripotent stem cell derived neurons in co culture with primary human astrocytes |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0303901&type=printable |
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