Concordance of Biochip-Based and LC-MS/MS Methods in Urine and Blood Samples in Screening for Amphetamine and Methamphetamine
<b>Objectives</b>: This study aimed to evaluate the efficacy and reliability of LC-MS/MS and biochip-based screening in detecting narcotics from blood and urine samples. <b>Materials and Methods</b>: In this single-center study, a total of 250 subjects provided urine and/or b...
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MDPI AG
2025-01-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/15/3/269 |
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| author | Murat Akbaba Aysun Baransel Isir |
| author_facet | Murat Akbaba Aysun Baransel Isir |
| author_sort | Murat Akbaba |
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| description | <b>Objectives</b>: This study aimed to evaluate the efficacy and reliability of LC-MS/MS and biochip-based screening in detecting narcotics from blood and urine samples. <b>Materials and Methods</b>: In this single-center study, a total of 250 subjects provided urine and/or blood samples: 234 provided urine and blood samples, and 16 provided only blood samples. Biochip-based narcotics screening was performed on 234 urine and 16 blood samples, and all samples were analyzed using tandem liquid chromatography-mass spectrometry (LC-MS/MS). <b>Results</b>: The two most prevalent narcotics were methamphetamine and amphetamine, with both biochip-based array and LC-MS/MS. Cohen’s kappa correlation indicated a substantial agreement between LC-MS/MS and biochip-based screening for both methamphetamine (κ = 0.661, <i>p</i> <0.001) and amphetamine (κ = 0.663, <i>p</i> < 0.001). LC-MS/MS demonstrated near-perfect agreement between urine and blood samples for both methamphetamine (κ = 0.855, <i>p</i> <0.001) and amphetamine (κ = 0.881, <i>p</i> < 0.001). <b>Conclusions</b>: Biochip-based arrays are a valid method offering a faster, less material-intensive alternative to LC-MS/MS for substance detection in forensic examination. |
| format | Article |
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| institution | OA Journals |
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| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-668b0e2e71ea4b08b92a8b1fa9efc5df2025-08-20T02:12:24ZengMDPI AGDiagnostics2075-44182025-01-0115326910.3390/diagnostics15030269Concordance of Biochip-Based and LC-MS/MS Methods in Urine and Blood Samples in Screening for Amphetamine and MethamphetamineMurat Akbaba0Aysun Baransel Isir1Department of Forensic Medicine, Faculty of Medicine, Gaziantep University, 27310 Gaziantep, TurkeyDepartment of Forensic Medicine, Faculty of Medicine, Gaziantep University, 27310 Gaziantep, Turkey<b>Objectives</b>: This study aimed to evaluate the efficacy and reliability of LC-MS/MS and biochip-based screening in detecting narcotics from blood and urine samples. <b>Materials and Methods</b>: In this single-center study, a total of 250 subjects provided urine and/or blood samples: 234 provided urine and blood samples, and 16 provided only blood samples. Biochip-based narcotics screening was performed on 234 urine and 16 blood samples, and all samples were analyzed using tandem liquid chromatography-mass spectrometry (LC-MS/MS). <b>Results</b>: The two most prevalent narcotics were methamphetamine and amphetamine, with both biochip-based array and LC-MS/MS. Cohen’s kappa correlation indicated a substantial agreement between LC-MS/MS and biochip-based screening for both methamphetamine (κ = 0.661, <i>p</i> <0.001) and amphetamine (κ = 0.663, <i>p</i> < 0.001). LC-MS/MS demonstrated near-perfect agreement between urine and blood samples for both methamphetamine (κ = 0.855, <i>p</i> <0.001) and amphetamine (κ = 0.881, <i>p</i> < 0.001). <b>Conclusions</b>: Biochip-based arrays are a valid method offering a faster, less material-intensive alternative to LC-MS/MS for substance detection in forensic examination.https://www.mdpi.com/2075-4418/15/3/269biochipamphetaminesmethamphetaminesLC-MS/MSnarcotics |
| spellingShingle | Murat Akbaba Aysun Baransel Isir Concordance of Biochip-Based and LC-MS/MS Methods in Urine and Blood Samples in Screening for Amphetamine and Methamphetamine Diagnostics biochip amphetamines methamphetamines LC-MS/MS narcotics |
| title | Concordance of Biochip-Based and LC-MS/MS Methods in Urine and Blood Samples in Screening for Amphetamine and Methamphetamine |
| title_full | Concordance of Biochip-Based and LC-MS/MS Methods in Urine and Blood Samples in Screening for Amphetamine and Methamphetamine |
| title_fullStr | Concordance of Biochip-Based and LC-MS/MS Methods in Urine and Blood Samples in Screening for Amphetamine and Methamphetamine |
| title_full_unstemmed | Concordance of Biochip-Based and LC-MS/MS Methods in Urine and Blood Samples in Screening for Amphetamine and Methamphetamine |
| title_short | Concordance of Biochip-Based and LC-MS/MS Methods in Urine and Blood Samples in Screening for Amphetamine and Methamphetamine |
| title_sort | concordance of biochip based and lc ms ms methods in urine and blood samples in screening for amphetamine and methamphetamine |
| topic | biochip amphetamines methamphetamines LC-MS/MS narcotics |
| url | https://www.mdpi.com/2075-4418/15/3/269 |
| work_keys_str_mv | AT muratakbaba concordanceofbiochipbasedandlcmsmsmethodsinurineandbloodsamplesinscreeningforamphetamineandmethamphetamine AT aysunbaranselisir concordanceofbiochipbasedandlcmsmsmethodsinurineandbloodsamplesinscreeningforamphetamineandmethamphetamine |