Antioxidant activity of Jinxuan tea polysaccharide and its protective effect on intestinal injury induced by transport stress in chicks
IntroductionTransport stress (TS) is unavoidable for livestock and poultry in modern agriculture, which not only affects animal welfare, but also to the quality of meat products. Tea polysaccharide (TPS), one of the major bioactive components of tea leaves, has been shown to have anti-stress effects...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1610218/full |
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| author | Xueyan Sun Qiaoyi Zhou Jinjing Gao Shujuan Liu Feike Zhao Caijin Ling Binghu Fang |
| author_facet | Xueyan Sun Qiaoyi Zhou Jinjing Gao Shujuan Liu Feike Zhao Caijin Ling Binghu Fang |
| author_sort | Xueyan Sun |
| collection | DOAJ |
| description | IntroductionTransport stress (TS) is unavoidable for livestock and poultry in modern agriculture, which not only affects animal welfare, but also to the quality of meat products. Tea polysaccharide (TPS), one of the major bioactive components of tea leaves, has been shown to have anti-stress effects. However, the antagonistic effect of TPS on TS-induced intestinal damage in chicks is unclear.MethodsThe free radical scavenging and reducing abilities of TPS were determined by chemical methods. RAW264.7 macrophages were stimulated by H2O2 to construct cell oxidative damage model, and the effects of TPS on cell viability and antioxidant enzyme activity were determined. A chick transport stress model was established using the shaker to investigate the effect of TPS on intestinal damage in chick caused by transport stress.ResultsIn vitro test results showed that TPS had good free radical scavenging ability and reducing ability. Furthermore, TPS could significantly alleviate cell oxidative damage induced by H2O2. In vivo test results showed that the TS-induced intestinal pathological damage and oxidative damage were markedly alleviated by TPS. Furthermore, TS resulted in the elevated expression of heat shock factors, disrupted expression of heat shock proteins, and similarly disrupted expression of aquaporins in chicks. Surprisingly, chicks treated with TPS showed a significant decrease in the expression of heat shock factors and significant alleviation of the dysregulated expression of heat shock proteins and aquaporins. Analysis of the intestinal flora showed that TS resulted in reduced intestinal flora diversity and altered flora structure in chicks, whereas the intestinal flora was normalized after TPS intervention.DiscussionTPS has good antioxidant activity and significantly ameliorate TS-induced intestinal damage in chicks, suggesting that TPS could be developed and utilized as a feed additive. |
| format | Article |
| id | doaj-art-667dd6833a0e4db0a289271ff755d0da |
| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-667dd6833a0e4db0a289271ff755d0da2025-08-20T03:26:26ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-06-011210.3389/fvets.2025.16102181610218Antioxidant activity of Jinxuan tea polysaccharide and its protective effect on intestinal injury induced by transport stress in chicksXueyan Sun0Qiaoyi Zhou1Jinjing Gao2Shujuan Liu3Feike Zhao4Caijin Ling5Binghu Fang6National Reference Laboratory of Veterinary Drug Residues, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Tea Plant Resources Innovation and Utilization, Tea Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaNational Reference Laboratory of Veterinary Drug Residues, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaNational Reference Laboratory of Veterinary Drug Residues, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaNational Reference Laboratory of Veterinary Drug Residues, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Tea Plant Resources Innovation and Utilization, Tea Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaNational Reference Laboratory of Veterinary Drug Residues, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaIntroductionTransport stress (TS) is unavoidable for livestock and poultry in modern agriculture, which not only affects animal welfare, but also to the quality of meat products. Tea polysaccharide (TPS), one of the major bioactive components of tea leaves, has been shown to have anti-stress effects. However, the antagonistic effect of TPS on TS-induced intestinal damage in chicks is unclear.MethodsThe free radical scavenging and reducing abilities of TPS were determined by chemical methods. RAW264.7 macrophages were stimulated by H2O2 to construct cell oxidative damage model, and the effects of TPS on cell viability and antioxidant enzyme activity were determined. A chick transport stress model was established using the shaker to investigate the effect of TPS on intestinal damage in chick caused by transport stress.ResultsIn vitro test results showed that TPS had good free radical scavenging ability and reducing ability. Furthermore, TPS could significantly alleviate cell oxidative damage induced by H2O2. In vivo test results showed that the TS-induced intestinal pathological damage and oxidative damage were markedly alleviated by TPS. Furthermore, TS resulted in the elevated expression of heat shock factors, disrupted expression of heat shock proteins, and similarly disrupted expression of aquaporins in chicks. Surprisingly, chicks treated with TPS showed a significant decrease in the expression of heat shock factors and significant alleviation of the dysregulated expression of heat shock proteins and aquaporins. Analysis of the intestinal flora showed that TS resulted in reduced intestinal flora diversity and altered flora structure in chicks, whereas the intestinal flora was normalized after TPS intervention.DiscussionTPS has good antioxidant activity and significantly ameliorate TS-induced intestinal damage in chicks, suggesting that TPS could be developed and utilized as a feed additive.https://www.frontiersin.org/articles/10.3389/fvets.2025.1610218/fulltea polysaccharideantioxidant activitytransport stressintestinal injuryintestinal flora |
| spellingShingle | Xueyan Sun Qiaoyi Zhou Jinjing Gao Shujuan Liu Feike Zhao Caijin Ling Binghu Fang Antioxidant activity of Jinxuan tea polysaccharide and its protective effect on intestinal injury induced by transport stress in chicks Frontiers in Veterinary Science tea polysaccharide antioxidant activity transport stress intestinal injury intestinal flora |
| title | Antioxidant activity of Jinxuan tea polysaccharide and its protective effect on intestinal injury induced by transport stress in chicks |
| title_full | Antioxidant activity of Jinxuan tea polysaccharide and its protective effect on intestinal injury induced by transport stress in chicks |
| title_fullStr | Antioxidant activity of Jinxuan tea polysaccharide and its protective effect on intestinal injury induced by transport stress in chicks |
| title_full_unstemmed | Antioxidant activity of Jinxuan tea polysaccharide and its protective effect on intestinal injury induced by transport stress in chicks |
| title_short | Antioxidant activity of Jinxuan tea polysaccharide and its protective effect on intestinal injury induced by transport stress in chicks |
| title_sort | antioxidant activity of jinxuan tea polysaccharide and its protective effect on intestinal injury induced by transport stress in chicks |
| topic | tea polysaccharide antioxidant activity transport stress intestinal injury intestinal flora |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1610218/full |
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