First Report of the Anthracnose Pathogenic Agent on Walnut Fruits in China and Exploration of Its Biological Characteristics
Anthracnose is recognized as a significant agricultural disease. This study investigates the disease symptoms characterized by black dots on walnut fruits observed in the walnut orchards of Longnan City, Gansu Province, China, in June 2022. These symptoms resemble those of anthracnose reported in pr...
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MDPI AG
2025-03-01
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| Series: | Horticulturae |
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| author | Chen Zhou Jinhuan Chen Yonggang Liu Ning Luo Wei Guo Mingming Shi Huixia Li |
| author_facet | Chen Zhou Jinhuan Chen Yonggang Liu Ning Luo Wei Guo Mingming Shi Huixia Li |
| author_sort | Chen Zhou |
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| description | Anthracnose is recognized as a significant agricultural disease. This study investigates the disease symptoms characterized by black dots on walnut fruits observed in the walnut orchards of Longnan City, Gansu Province, China, in June 2022. These symptoms resemble those of anthracnose reported in previous studies. A strain designated Ht-10 was initially isolated and identified as belonging to the <i>Colletotrichum</i> species based on its morphological features. Pathogenicity tests confirmed that this strain induced pronounced anthracnose symptoms in walnuts, consistent with those originally observed in the field. Subsequently, multilocus phylogenetic analysis, which included partial sequences of the internal transcribed spacer (ITS), actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), β-tubulin2 (TUB2), and chitin synthase (CHS-1) genes of Ht-10, indicated that it most likely clustered with <i>Colletotrichum fioriniae</i>. The determination of biological characteristics revealed that the optimal temperature for the growth of Ht-10 was 25 °C in full light at a pH of 6, with soluble starch and yeast paste serving as the optimal carbon and nitrogen sources, respectively. To our knowledge, this is the first report of <i>C. fioriniae</i> as a causal agent of anthracnose in walnut fruits in China. |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-03-01 |
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| series | Horticulturae |
| spelling | doaj-art-667daaf161f64aa2bba93fc2b098e99a2025-08-20T03:43:34ZengMDPI AGHorticulturae2311-75242025-03-0111333910.3390/horticulturae11030339First Report of the Anthracnose Pathogenic Agent on Walnut Fruits in China and Exploration of Its Biological CharacteristicsChen Zhou0Jinhuan Chen1Yonggang Liu2Ning Luo3Wei Guo4Mingming Shi5Huixia Li6Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, ChinaBiocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, ChinaInstitute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou 730070, ChinaBiocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, ChinaBiocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, ChinaBiocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, ChinaBiocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, ChinaAnthracnose is recognized as a significant agricultural disease. This study investigates the disease symptoms characterized by black dots on walnut fruits observed in the walnut orchards of Longnan City, Gansu Province, China, in June 2022. These symptoms resemble those of anthracnose reported in previous studies. A strain designated Ht-10 was initially isolated and identified as belonging to the <i>Colletotrichum</i> species based on its morphological features. Pathogenicity tests confirmed that this strain induced pronounced anthracnose symptoms in walnuts, consistent with those originally observed in the field. Subsequently, multilocus phylogenetic analysis, which included partial sequences of the internal transcribed spacer (ITS), actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), β-tubulin2 (TUB2), and chitin synthase (CHS-1) genes of Ht-10, indicated that it most likely clustered with <i>Colletotrichum fioriniae</i>. The determination of biological characteristics revealed that the optimal temperature for the growth of Ht-10 was 25 °C in full light at a pH of 6, with soluble starch and yeast paste serving as the optimal carbon and nitrogen sources, respectively. To our knowledge, this is the first report of <i>C. fioriniae</i> as a causal agent of anthracnose in walnut fruits in China.https://www.mdpi.com/2311-7524/11/3/339walnutanthracnose<i>Colletotrichum fioriniae</i>isolationidentification |
| spellingShingle | Chen Zhou Jinhuan Chen Yonggang Liu Ning Luo Wei Guo Mingming Shi Huixia Li First Report of the Anthracnose Pathogenic Agent on Walnut Fruits in China and Exploration of Its Biological Characteristics Horticulturae walnut anthracnose <i>Colletotrichum fioriniae</i> isolation identification |
| title | First Report of the Anthracnose Pathogenic Agent on Walnut Fruits in China and Exploration of Its Biological Characteristics |
| title_full | First Report of the Anthracnose Pathogenic Agent on Walnut Fruits in China and Exploration of Its Biological Characteristics |
| title_fullStr | First Report of the Anthracnose Pathogenic Agent on Walnut Fruits in China and Exploration of Its Biological Characteristics |
| title_full_unstemmed | First Report of the Anthracnose Pathogenic Agent on Walnut Fruits in China and Exploration of Its Biological Characteristics |
| title_short | First Report of the Anthracnose Pathogenic Agent on Walnut Fruits in China and Exploration of Its Biological Characteristics |
| title_sort | first report of the anthracnose pathogenic agent on walnut fruits in china and exploration of its biological characteristics |
| topic | walnut anthracnose <i>Colletotrichum fioriniae</i> isolation identification |
| url | https://www.mdpi.com/2311-7524/11/3/339 |
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