Automation of multiplex biochemical assays to enhance productivity and reduce cycle time using a modular robotic platform
Pharmaceutical and biotechnology companies are increasingly being challenged to shorten the cycle time between design, make, test, and analyze (DMTA) compounds. Automation of multiplex assays to obtain multiparameter data on the same robotic run is instrumental in reducing cycle time. Consequently,...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | SLAS Technology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2472630324001158 |
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| author | Buyun Tang Becky Lam Stephanie Holley Martha Torres Theresa Kuntzweiler Tatiana Gladysheva Paul Lang |
| author_facet | Buyun Tang Becky Lam Stephanie Holley Martha Torres Theresa Kuntzweiler Tatiana Gladysheva Paul Lang |
| author_sort | Buyun Tang |
| collection | DOAJ |
| description | Pharmaceutical and biotechnology companies are increasingly being challenged to shorten the cycle time between design, make, test, and analyze (DMTA) compounds. Automation of multiplex assays to obtain multiparameter data on the same robotic run is instrumental in reducing cycle time. Consequently, an increasing need in automated systems to streamline laboratory workflows with the goal to expedite assay cycle time and enhance productivity has grown in industrial and academic institutions in the past decades. Herein, we present a customized robotic platform with operational modularity and flexibility, designed to automate entire assay workflows involving multistep reagent dispensing, mixing, lidding/de-lidding, incubation, centrifugation, and final readout steps by linking spinnaker robot with various peripheral instruments. Compared to manual workflows, the system can seamlessly execute processes with high efficiency, evaluated by standard assay validation protocols for robustness and reproducibility. Furthermore, the system can perform multiple, independent protocols in parallel, and has high-throughput capacity. In this publication, we demonstrate that the modular robotic platform can fully automate multiplex assay workflows through ‘one-click-and-run’ solution with tremendous benefits in liberating manual intervention, boosting productivity while producing high-quality data combined with reduced cycle time (>20 %), as well as expanding the capacity for higher throughput. |
| format | Article |
| id | doaj-art-667b3d6cd55948858c5f1cd5d51f9adb |
| institution | OA Journals |
| issn | 2472-6303 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | SLAS Technology |
| spelling | doaj-art-667b3d6cd55948858c5f1cd5d51f9adb2025-08-20T02:36:58ZengElsevierSLAS Technology2472-63032024-12-0129610023310.1016/j.slast.2024.100233Automation of multiplex biochemical assays to enhance productivity and reduce cycle time using a modular robotic platformBuyun Tang0Becky Lam1Stephanie Holley2Martha Torres3Theresa Kuntzweiler4Tatiana Gladysheva5Paul Lang6Corresponding author.; Sanofi Integrated Drug Discovery, 350 Water St, Cambridge, MA, USASanofi Integrated Drug Discovery, 350 Water St, Cambridge, MA, USASanofi Integrated Drug Discovery, 350 Water St, Cambridge, MA, USASanofi Integrated Drug Discovery, 350 Water St, Cambridge, MA, USASanofi Integrated Drug Discovery, 350 Water St, Cambridge, MA, USASanofi Integrated Drug Discovery, 350 Water St, Cambridge, MA, USASanofi Integrated Drug Discovery, 350 Water St, Cambridge, MA, USAPharmaceutical and biotechnology companies are increasingly being challenged to shorten the cycle time between design, make, test, and analyze (DMTA) compounds. Automation of multiplex assays to obtain multiparameter data on the same robotic run is instrumental in reducing cycle time. Consequently, an increasing need in automated systems to streamline laboratory workflows with the goal to expedite assay cycle time and enhance productivity has grown in industrial and academic institutions in the past decades. Herein, we present a customized robotic platform with operational modularity and flexibility, designed to automate entire assay workflows involving multistep reagent dispensing, mixing, lidding/de-lidding, incubation, centrifugation, and final readout steps by linking spinnaker robot with various peripheral instruments. Compared to manual workflows, the system can seamlessly execute processes with high efficiency, evaluated by standard assay validation protocols for robustness and reproducibility. Furthermore, the system can perform multiple, independent protocols in parallel, and has high-throughput capacity. In this publication, we demonstrate that the modular robotic platform can fully automate multiplex assay workflows through ‘one-click-and-run’ solution with tremendous benefits in liberating manual intervention, boosting productivity while producing high-quality data combined with reduced cycle time (>20 %), as well as expanding the capacity for higher throughput.http://www.sciencedirect.com/science/article/pii/S2472630324001158Laboratory automationDrug discoveryMultiplex assaysCycle time |
| spellingShingle | Buyun Tang Becky Lam Stephanie Holley Martha Torres Theresa Kuntzweiler Tatiana Gladysheva Paul Lang Automation of multiplex biochemical assays to enhance productivity and reduce cycle time using a modular robotic platform SLAS Technology Laboratory automation Drug discovery Multiplex assays Cycle time |
| title | Automation of multiplex biochemical assays to enhance productivity and reduce cycle time using a modular robotic platform |
| title_full | Automation of multiplex biochemical assays to enhance productivity and reduce cycle time using a modular robotic platform |
| title_fullStr | Automation of multiplex biochemical assays to enhance productivity and reduce cycle time using a modular robotic platform |
| title_full_unstemmed | Automation of multiplex biochemical assays to enhance productivity and reduce cycle time using a modular robotic platform |
| title_short | Automation of multiplex biochemical assays to enhance productivity and reduce cycle time using a modular robotic platform |
| title_sort | automation of multiplex biochemical assays to enhance productivity and reduce cycle time using a modular robotic platform |
| topic | Laboratory automation Drug discovery Multiplex assays Cycle time |
| url | http://www.sciencedirect.com/science/article/pii/S2472630324001158 |
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