Optimization of Wall-disruption Process of Rose Bee Pollen and Comparison of Physiological Activities of Its Ethanol Extracts
Rose bee pollen has a pleasant aroma and a sweet taste, and it is rich in polyphenols and other bioactive substances, making it a highly nutritious and promising natural health product. To optimize the enzymatic hydrolysis process for rose bee pollen and to systematically compare the physiological a...
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The editorial department of Science and Technology of Food Industry
2025-02-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024030101 |
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| author | Xinyang LU Yishu SUN Tao XU Longbin FAN Yaning CHANG Yingjun ZHOU |
| author_facet | Xinyang LU Yishu SUN Tao XU Longbin FAN Yaning CHANG Yingjun ZHOU |
| author_sort | Xinyang LU |
| collection | DOAJ |
| description | Rose bee pollen has a pleasant aroma and a sweet taste, and it is rich in polyphenols and other bioactive substances, making it a highly nutritious and promising natural health product. To optimize the enzymatic hydrolysis process for rose bee pollen and to systematically compare the physiological activities and primary component profiles of its different ethanolic extracts. This was done with the aim of discerning the most efficacious extract that possessed significant anti-inflammatory and anti-benign prostatic hyperplasia (BPH) effects. The results demonstrated that the optimal enzymatic hydrolysis process parameters for rose bee pollen were a solid-to-liquid ratio of 1:30, a temperature of 55 °C, pH6, a composite enzyme system addition of 2.0% (cellulase comprising a ratio of 7:5 with composite protease), and a reaction time of 9 h, resulting in the highest wall-disruption rate of 73.13%. The active substances extracted with 90% ethanol could significantly (P<0.01) reduce the release level of inflammatory factors induced by lipopolysaccharide in RAW264.7 cells (at 20 μg/mL, compared with the model group, nitric oxide, interleukin-6 and tumor necrosis factor-α decreased by 67.04%, 16.74% and 21.74% respectively), and had the most obvious inhibitory effect on the activity and proliferation of BPH-1 cells, thus exhibiting optimal anti-inflammatory and anti-BPH effects. This research provides a theoretical basis for identifying the active ingredients in rose bee pollen and further developing value-added processed products. |
| format | Article |
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| institution | Kabale University |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-02-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj-art-66718e5e95c04adf9be28063da9399cf2025-02-08T08:06:21ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-02-0146417618410.13386/j.issn1002-0306.20240301012024030101-4Optimization of Wall-disruption Process of Rose Bee Pollen and Comparison of Physiological Activities of Its Ethanol ExtractsXinyang LU0Yishu SUN1Tao XU2Longbin FAN3Yaning CHANG4Yingjun ZHOU5College of Biotechnology, East China University of Science and Technology, Shanghai 200237, ChinaCollege of Biotechnology, East China University of Science and Technology, Shanghai 200237, ChinaHuzhou Shengtao Biotechnology Co., Ltd., Huzhou 313000, ChinaShanghai Hongcheng Industrial Development Co., Ltd., Shanghai 200232, ChinaCollege of Biotechnology, East China University of Science and Technology, Shanghai 200237, ChinaCollege of Biotechnology, East China University of Science and Technology, Shanghai 200237, ChinaRose bee pollen has a pleasant aroma and a sweet taste, and it is rich in polyphenols and other bioactive substances, making it a highly nutritious and promising natural health product. To optimize the enzymatic hydrolysis process for rose bee pollen and to systematically compare the physiological activities and primary component profiles of its different ethanolic extracts. This was done with the aim of discerning the most efficacious extract that possessed significant anti-inflammatory and anti-benign prostatic hyperplasia (BPH) effects. The results demonstrated that the optimal enzymatic hydrolysis process parameters for rose bee pollen were a solid-to-liquid ratio of 1:30, a temperature of 55 °C, pH6, a composite enzyme system addition of 2.0% (cellulase comprising a ratio of 7:5 with composite protease), and a reaction time of 9 h, resulting in the highest wall-disruption rate of 73.13%. The active substances extracted with 90% ethanol could significantly (P<0.01) reduce the release level of inflammatory factors induced by lipopolysaccharide in RAW264.7 cells (at 20 μg/mL, compared with the model group, nitric oxide, interleukin-6 and tumor necrosis factor-α decreased by 67.04%, 16.74% and 21.74% respectively), and had the most obvious inhibitory effect on the activity and proliferation of BPH-1 cells, thus exhibiting optimal anti-inflammatory and anti-BPH effects. This research provides a theoretical basis for identifying the active ingredients in rose bee pollen and further developing value-added processed products.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024030101rose bee pollenwall-disruptionethanol extractsanti-inflammatorybenign prostatic hyperplasia |
| spellingShingle | Xinyang LU Yishu SUN Tao XU Longbin FAN Yaning CHANG Yingjun ZHOU Optimization of Wall-disruption Process of Rose Bee Pollen and Comparison of Physiological Activities of Its Ethanol Extracts Shipin gongye ke-ji rose bee pollen wall-disruption ethanol extracts anti-inflammatory benign prostatic hyperplasia |
| title | Optimization of Wall-disruption Process of Rose Bee Pollen and Comparison of Physiological Activities of Its Ethanol Extracts |
| title_full | Optimization of Wall-disruption Process of Rose Bee Pollen and Comparison of Physiological Activities of Its Ethanol Extracts |
| title_fullStr | Optimization of Wall-disruption Process of Rose Bee Pollen and Comparison of Physiological Activities of Its Ethanol Extracts |
| title_full_unstemmed | Optimization of Wall-disruption Process of Rose Bee Pollen and Comparison of Physiological Activities of Its Ethanol Extracts |
| title_short | Optimization of Wall-disruption Process of Rose Bee Pollen and Comparison of Physiological Activities of Its Ethanol Extracts |
| title_sort | optimization of wall disruption process of rose bee pollen and comparison of physiological activities of its ethanol extracts |
| topic | rose bee pollen wall-disruption ethanol extracts anti-inflammatory benign prostatic hyperplasia |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024030101 |
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