Comparison and phylogenetic analysis of the mitochondrial genomes of Synodontis eupterus and Synodontis polli
Abstract We aimed to distinguish Synodontis eupterus and Synodontis polli. We performed sequencing and bioinformatic analysis of their mitochondrial genomes and constructed a phylogenetic tree of Mochokidae fish using maximum likelihood and Bayesian methods based on protein-coding gene (PCG) sequenc...
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Nature Portfolio
2024-07-01
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| Online Access: | https://doi.org/10.1038/s41598-024-65809-4 |
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| author | Cheng-He Sun Chang-Hu Lu Zi-Jian Wang |
| author_facet | Cheng-He Sun Chang-Hu Lu Zi-Jian Wang |
| author_sort | Cheng-He Sun |
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| description | Abstract We aimed to distinguish Synodontis eupterus and Synodontis polli. We performed sequencing and bioinformatic analysis of their mitochondrial genomes and constructed a phylogenetic tree of Mochokidae fish using maximum likelihood and Bayesian methods based on protein-coding gene (PCG) sequences of 14 Mochokidae species. The total length of the S. eupterus mitochondrial genome was 16,579 bp, including 13 (PCGs), 22 tRNA genes, two rRNA genes, and one D-loop, with an AT-biased nucleotide composition (56.0%). The total length of the S. polli mitochondrial genome was 16,544 bp, including 13 PCGs, 22 tRNA genes, two rRNA genes, and one D-loop, with an AT-biased nucleotide composition (55.0%). In both species, except for COI, PCGs use ATG as the starting codon, the vast majority use TAG or TAA as the ending codon, and a few use incomplete codons (T - or TA -) as the ending codon. Phylogenetic analysis showed that S. eupterus and Synodontis clarias converged into one branch, S. polli and Synodontis petricola converged into one branch, Mochokiella paynei, Mochokus brevis, and nine species of the genus Synodontis converged into one branch, and M. paynei clustered with the genus Synodontis. This study lays a foundation for rebuilding a clearer Mochokidae fish classification system. |
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| id | doaj-art-6660c8aac200410d9cab549572aa2339 |
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| language | English |
| publishDate | 2024-07-01 |
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| spelling | doaj-art-6660c8aac200410d9cab549572aa23392025-08-20T02:35:39ZengNature PortfolioScientific Reports2045-23222024-07-0114111110.1038/s41598-024-65809-4Comparison and phylogenetic analysis of the mitochondrial genomes of Synodontis eupterus and Synodontis polliCheng-He Sun0Chang-Hu Lu1Zi-Jian Wang2The Co-Innovation Center for Sustainable Forestry in Southern China, College of Life Sciences, Nanjing Forestry UniversityThe Co-Innovation Center for Sustainable Forestry in Southern China, College of Life Sciences, Nanjing Forestry UniversityAgriculture and Rural Bureau of Gaochun DistrictAbstract We aimed to distinguish Synodontis eupterus and Synodontis polli. We performed sequencing and bioinformatic analysis of their mitochondrial genomes and constructed a phylogenetic tree of Mochokidae fish using maximum likelihood and Bayesian methods based on protein-coding gene (PCG) sequences of 14 Mochokidae species. The total length of the S. eupterus mitochondrial genome was 16,579 bp, including 13 (PCGs), 22 tRNA genes, two rRNA genes, and one D-loop, with an AT-biased nucleotide composition (56.0%). The total length of the S. polli mitochondrial genome was 16,544 bp, including 13 PCGs, 22 tRNA genes, two rRNA genes, and one D-loop, with an AT-biased nucleotide composition (55.0%). In both species, except for COI, PCGs use ATG as the starting codon, the vast majority use TAG or TAA as the ending codon, and a few use incomplete codons (T - or TA -) as the ending codon. Phylogenetic analysis showed that S. eupterus and Synodontis clarias converged into one branch, S. polli and Synodontis petricola converged into one branch, Mochokiella paynei, Mochokus brevis, and nine species of the genus Synodontis converged into one branch, and M. paynei clustered with the genus Synodontis. This study lays a foundation for rebuilding a clearer Mochokidae fish classification system.https://doi.org/10.1038/s41598-024-65809-4MochokidaeSynodontisMitochondrial genomePhylogenetic |
| spellingShingle | Cheng-He Sun Chang-Hu Lu Zi-Jian Wang Comparison and phylogenetic analysis of the mitochondrial genomes of Synodontis eupterus and Synodontis polli Scientific Reports Mochokidae Synodontis Mitochondrial genome Phylogenetic |
| title | Comparison and phylogenetic analysis of the mitochondrial genomes of Synodontis eupterus and Synodontis polli |
| title_full | Comparison and phylogenetic analysis of the mitochondrial genomes of Synodontis eupterus and Synodontis polli |
| title_fullStr | Comparison and phylogenetic analysis of the mitochondrial genomes of Synodontis eupterus and Synodontis polli |
| title_full_unstemmed | Comparison and phylogenetic analysis of the mitochondrial genomes of Synodontis eupterus and Synodontis polli |
| title_short | Comparison and phylogenetic analysis of the mitochondrial genomes of Synodontis eupterus and Synodontis polli |
| title_sort | comparison and phylogenetic analysis of the mitochondrial genomes of synodontis eupterus and synodontis polli |
| topic | Mochokidae Synodontis Mitochondrial genome Phylogenetic |
| url | https://doi.org/10.1038/s41598-024-65809-4 |
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