Paeoniflorin inhibits the growth of bladder carcinoma via deactivation of STAT3
Bladder cancer (BCa) is one of the most common urinary cancers. The present study aims to investigate whether Paeoniflorin (Pae) can exert inhibitory effects on BCa. The results showed that Pae inhibited proliferation of human BCa cell lines in a concentration- and time-dependent manner. Pae and cis...
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| Format: | Article |
| Language: | English |
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Sciendo
2018-06-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2018-0013 |
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| author | Yang Jianhui Ren Yu Lou Zhong-Guan Wan Xue Weng Guo-Bin Cen Dong |
| author_facet | Yang Jianhui Ren Yu Lou Zhong-Guan Wan Xue Weng Guo-Bin Cen Dong |
| author_sort | Yang Jianhui |
| collection | DOAJ |
| description | Bladder cancer (BCa) is one of the most common urinary cancers. The present study aims to investigate whether Paeoniflorin (Pae) can exert inhibitory effects on BCa. The results showed that Pae inhibited proliferation of human BCa cell lines in a concentration- and time-dependent manner. Pae and cisplatin (Cis) synergistically inhibited the growth of tumours in RT4-bearing mice. Pae treatment neutralized the body loss induced by Cis. Moreover, Pae induced apoptosis in RT4 cells and increased the activities of caspase3, caspase8 and caspase9. Western blotting and immunohistochemical analysis revealed that the phosphorylated signal transducer and activator of transcription-3 (p-STAT3) level were decreased in Pae-treated RT4 cells and Pae-treated tumour-bearing mice. Furthermore, STAT3 transcriptional target B-cell lymphoma-2 was decreased in Pae-treated RT4 cells. Interestingly, Pae prevented translocation of STAT3 to the nucleus in RT4 cells. Collectively, Pae inhibits the growth of BCa, at least in part, via a STAT3 pathway. |
| format | Article |
| id | doaj-art-665d4ee21cc147c08002ede81041810a |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2018-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-665d4ee21cc147c08002ede81041810a2025-02-02T11:49:57ZengSciendoActa Pharmaceutica1846-95582018-06-0168221122210.2478/acph-2018-0013acph-2018-0013Paeoniflorin inhibits the growth of bladder carcinoma via deactivation of STAT3Yang Jianhui0Ren Yu1Lou Zhong-Guan2Wan Xue3Weng Guo-Bin4Cen Dong5Laboratory of Kidney Carcinoma, Ningbo Urology and Nephrology Hospital, Urology and Nephrology Institute of Ningbo University, Ningbo, Zhejiang 315000, P.R. ChinaLaboratory of Kidney Carcinoma, Ningbo Urology and Nephrology Hospital, Urology and Nephrology Institute of Ningbo University, Ningbo, Zhejiang 315000, P.R. ChinaLaboratory of Kidney Carcinoma, Ningbo Urology and Nephrology Hospital, Urology and Nephrology Institute of Ningbo University, Ningbo, Zhejiang 315000, P.R. ChinaLaboratory of Kidney Carcinoma, Ningbo Urology and Nephrology Hospital, Urology and Nephrology Institute of Ningbo University, Ningbo, Zhejiang 315000, P.R. ChinaLaboratory of Kidney Carcinoma, Ningbo Urology and Nephrology Hospital, Urology and Nephrology Institute of Ningbo University, Ningbo, Zhejiang 315000, P.R. ChinaLaboratory of Kidney Carcinoma, Ningbo Urology and Nephrology Hospital, Urology and Nephrology Institute of Ningbo University, Ningbo, Zhejiang 315000, P.R. ChinaBladder cancer (BCa) is one of the most common urinary cancers. The present study aims to investigate whether Paeoniflorin (Pae) can exert inhibitory effects on BCa. The results showed that Pae inhibited proliferation of human BCa cell lines in a concentration- and time-dependent manner. Pae and cisplatin (Cis) synergistically inhibited the growth of tumours in RT4-bearing mice. Pae treatment neutralized the body loss induced by Cis. Moreover, Pae induced apoptosis in RT4 cells and increased the activities of caspase3, caspase8 and caspase9. Western blotting and immunohistochemical analysis revealed that the phosphorylated signal transducer and activator of transcription-3 (p-STAT3) level were decreased in Pae-treated RT4 cells and Pae-treated tumour-bearing mice. Furthermore, STAT3 transcriptional target B-cell lymphoma-2 was decreased in Pae-treated RT4 cells. Interestingly, Pae prevented translocation of STAT3 to the nucleus in RT4 cells. Collectively, Pae inhibits the growth of BCa, at least in part, via a STAT3 pathway.https://doi.org/10.2478/acph-2018-0013bladder cancerradix paeoniae albapaeoniflorinapoptosisstat3 |
| spellingShingle | Yang Jianhui Ren Yu Lou Zhong-Guan Wan Xue Weng Guo-Bin Cen Dong Paeoniflorin inhibits the growth of bladder carcinoma via deactivation of STAT3 Acta Pharmaceutica bladder cancer radix paeoniae alba paeoniflorin apoptosis stat3 |
| title | Paeoniflorin inhibits the growth of bladder carcinoma via deactivation of STAT3 |
| title_full | Paeoniflorin inhibits the growth of bladder carcinoma via deactivation of STAT3 |
| title_fullStr | Paeoniflorin inhibits the growth of bladder carcinoma via deactivation of STAT3 |
| title_full_unstemmed | Paeoniflorin inhibits the growth of bladder carcinoma via deactivation of STAT3 |
| title_short | Paeoniflorin inhibits the growth of bladder carcinoma via deactivation of STAT3 |
| title_sort | paeoniflorin inhibits the growth of bladder carcinoma via deactivation of stat3 |
| topic | bladder cancer radix paeoniae alba paeoniflorin apoptosis stat3 |
| url | https://doi.org/10.2478/acph-2018-0013 |
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