Possibilities of investigating intratumoral lymphocyte subpopulations by flow cytometry of tumor core biopsy specimens in patients with breast cancer
Study of tumor-infiltrating lymphocyte subpopulations was performed in 19 patients with breast cancer (core biopsy material). Two probes were used in multiparameter flow cytometry: CD4/CD25/CD3/CD45 and CD8/CD56/CD3/CD45. Mean percentage of different cell types within lymphocytes (CD45++ SSCLow) was...
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| Format: | Article |
| Language: | Russian |
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ABV-press
2014-07-01
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| Series: | Опухоли женской репродуктивной системы |
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| Online Access: | https://ojrs.abvpress.ru/ojrs/article/view/111 |
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| author | F. A. Shamilov Ya. V. Vishnevskaya V. Yu. Selchuk E. M. Pogodina D. I. Zernov N. V. Chkhikvadze V. V. Timoshenko D. A. Ryabchikov N. N. Tupitsyn |
| author_facet | F. A. Shamilov Ya. V. Vishnevskaya V. Yu. Selchuk E. M. Pogodina D. I. Zernov N. V. Chkhikvadze V. V. Timoshenko D. A. Ryabchikov N. N. Tupitsyn |
| author_sort | F. A. Shamilov |
| collection | DOAJ |
| description | Study of tumor-infiltrating lymphocyte subpopulations was performed in 19 patients with breast cancer (core biopsy material). Two probes were used in multiparameter flow cytometry: CD4/CD25/CD3/CD45 and CD8/CD56/CD3/CD45. Mean percentage of different cell types within lymphocytes (CD45++ SSCLow) was as follows: CD3 – 88.2%, CD4 – 45.1%, CD8 – 40.4%, NK (CD3–CD56+) – 1.6%, CD4+CD25+ – 4.1 %. Ratio CD8/CD4 was less than 1 in 53 % of cases. In cases of prominent and moderate CD45 infiltration percentage of CD8+ lymphocytes was significantly higher than in cases with low infiltration, p = 0.007. |
| format | Article |
| id | doaj-art-665a08df68bf41e7971a08cf198dc155 |
| institution | Kabale University |
| issn | 1994-4098 1999-8627 |
| language | Russian |
| publishDate | 2014-07-01 |
| publisher | ABV-press |
| record_format | Article |
| series | Опухоли женской репродуктивной системы |
| spelling | doaj-art-665a08df68bf41e7971a08cf198dc1552025-08-20T04:00:02ZrusABV-pressОпухоли женской репродуктивной системы1994-40981999-86272014-07-0103-4293310.17650/1994-4098-2012-0-3-4-29-33127Possibilities of investigating intratumoral lymphocyte subpopulations by flow cytometry of tumor core biopsy specimens in patients with breast cancerF. A. Shamilov0Ya. V. Vishnevskaya1V. Yu. Selchuk2E. M. Pogodina3D. I. Zernov4N. V. Chkhikvadze5V. V. Timoshenko6D. A. Ryabchikov7N. N. Tupitsyn8Department of Oncology, Moscow State University of Medicine and DentistryN.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, MoscowDepartment of Oncology, Moscow State University of Medicine and DentistryN.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, MoscowN.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, MoscowN.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, MoscowN.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, MoscowN.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, MoscowN.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, MoscowStudy of tumor-infiltrating lymphocyte subpopulations was performed in 19 patients with breast cancer (core biopsy material). Two probes were used in multiparameter flow cytometry: CD4/CD25/CD3/CD45 and CD8/CD56/CD3/CD45. Mean percentage of different cell types within lymphocytes (CD45++ SSCLow) was as follows: CD3 – 88.2%, CD4 – 45.1%, CD8 – 40.4%, NK (CD3–CD56+) – 1.6%, CD4+CD25+ – 4.1 %. Ratio CD8/CD4 was less than 1 in 53 % of cases. In cases of prominent and moderate CD45 infiltration percentage of CD8+ lymphocytes was significantly higher than in cases with low infiltration, p = 0.007.https://ojrs.abvpress.ru/ojrs/article/view/111breast cancersubpopulations of tumor-infiltrating lymphocytescore-biopsyflow cytometry |
| spellingShingle | F. A. Shamilov Ya. V. Vishnevskaya V. Yu. Selchuk E. M. Pogodina D. I. Zernov N. V. Chkhikvadze V. V. Timoshenko D. A. Ryabchikov N. N. Tupitsyn Possibilities of investigating intratumoral lymphocyte subpopulations by flow cytometry of tumor core biopsy specimens in patients with breast cancer Опухоли женской репродуктивной системы breast cancer subpopulations of tumor-infiltrating lymphocytes core-biopsy flow cytometry |
| title | Possibilities of investigating intratumoral lymphocyte subpopulations by flow cytometry of tumor core biopsy specimens in patients with breast cancer |
| title_full | Possibilities of investigating intratumoral lymphocyte subpopulations by flow cytometry of tumor core biopsy specimens in patients with breast cancer |
| title_fullStr | Possibilities of investigating intratumoral lymphocyte subpopulations by flow cytometry of tumor core biopsy specimens in patients with breast cancer |
| title_full_unstemmed | Possibilities of investigating intratumoral lymphocyte subpopulations by flow cytometry of tumor core biopsy specimens in patients with breast cancer |
| title_short | Possibilities of investigating intratumoral lymphocyte subpopulations by flow cytometry of tumor core biopsy specimens in patients with breast cancer |
| title_sort | possibilities of investigating intratumoral lymphocyte subpopulations by flow cytometry of tumor core biopsy specimens in patients with breast cancer |
| topic | breast cancer subpopulations of tumor-infiltrating lymphocytes core-biopsy flow cytometry |
| url | https://ojrs.abvpress.ru/ojrs/article/view/111 |
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