Resistance to colistin and various antibiotics in non-typhoidal Salmonella
BACKGROUND: Non-typhoidal Salmonella has been reported as one of the four major global causes of diarrheal diseases by the World Health Organization. In recent years, Salmonella strains resistant to both antibiotics used in treatment and colistin have been increasing worldwide, and therefore resista...
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Elsevier
2024-12-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716524002431 |
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| author | Rumeysa Bildik Betigül Öngen |
| author_facet | Rumeysa Bildik Betigül Öngen |
| author_sort | Rumeysa Bildik |
| collection | DOAJ |
| description | BACKGROUND: Non-typhoidal Salmonella has been reported as one of the four major global causes of diarrheal diseases by the World Health Organization. In recent years, Salmonella strains resistant to both antibiotics used in treatment and colistin have been increasing worldwide, and therefore resistance is monitored on a country-by-country basis. In this study, it was aimed to determine the minimal inhibitor concentration (MIC) values of antibiotics frequently used in the treatment and of colistin and to investigate plasmid-mediated mcr genes that play a role in colistin resistance. METHODS: MIC values for colistin (COL), ampicillin (AMP), trimethoprim-sulfamethoxazole (SXT), ciprofloxacin (CIP) and ceftriaxone (CRO) were determined by broth microdilution method in 83 Salmonella (70 S. Enteritidis, 13 S. Typhimurium) isolates from patients with gastroenteritis in accordance with the European Committee on Antimicrobial Susceptibility Testing recommendations and mcr-1 and mcr-2 genes were investigated by multiplex PCR method. RESULTS: Resistance to COL, CIP and AMP were found 13.25%, 4.81% and 1.2%, respectively. All Salmonella strains were susceptible to SXT and CRO. MIC ranges and MIC50-MIC90 values were found 0.25-8 mg/L and 1-4 mg/L for COL; 1-32 mg/L and 2-2 mg/L for AMP; 0.06-0.5 mg/L and 0.125-0.125 mg/L for SXT; 0.008-0.5 mg/L and 0.016-0.03 mg/L for CIP and 0.03-0.5 mg/L and 0.06-0.125 mg/L for CRO, respectively. The mcr-1 and mcr-2 genes were not detected in Salmonella isolates. CONCLUSIONS: High-level and multi-resistance were not observed in our isolates. While resistance was generally low, the highest resistance rate was observed against colistin. |
| format | Article |
| id | doaj-art-664e2a4470b447fa9ecaa705c9ce7ada |
| institution | DOAJ |
| issn | 2213-7165 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj-art-664e2a4470b447fa9ecaa705c9ce7ada2025-08-20T02:53:16ZengElsevierJournal of Global Antimicrobial Resistance2213-71652024-12-0139212210.1016/j.jgar.2024.10.066Resistance to colistin and various antibiotics in non-typhoidal SalmonellaRumeysa Bildik0Betigül Öngen1Istanbul University, Istanbul Faculty of Medicine, Department of Medical Microbiology, Istanbul, TurkeyIstanbul University, Istanbul Faculty of Medicine, Department of Medical Microbiology, Istanbul, TurkeyBACKGROUND: Non-typhoidal Salmonella has been reported as one of the four major global causes of diarrheal diseases by the World Health Organization. In recent years, Salmonella strains resistant to both antibiotics used in treatment and colistin have been increasing worldwide, and therefore resistance is monitored on a country-by-country basis. In this study, it was aimed to determine the minimal inhibitor concentration (MIC) values of antibiotics frequently used in the treatment and of colistin and to investigate plasmid-mediated mcr genes that play a role in colistin resistance. METHODS: MIC values for colistin (COL), ampicillin (AMP), trimethoprim-sulfamethoxazole (SXT), ciprofloxacin (CIP) and ceftriaxone (CRO) were determined by broth microdilution method in 83 Salmonella (70 S. Enteritidis, 13 S. Typhimurium) isolates from patients with gastroenteritis in accordance with the European Committee on Antimicrobial Susceptibility Testing recommendations and mcr-1 and mcr-2 genes were investigated by multiplex PCR method. RESULTS: Resistance to COL, CIP and AMP were found 13.25%, 4.81% and 1.2%, respectively. All Salmonella strains were susceptible to SXT and CRO. MIC ranges and MIC50-MIC90 values were found 0.25-8 mg/L and 1-4 mg/L for COL; 1-32 mg/L and 2-2 mg/L for AMP; 0.06-0.5 mg/L and 0.125-0.125 mg/L for SXT; 0.008-0.5 mg/L and 0.016-0.03 mg/L for CIP and 0.03-0.5 mg/L and 0.06-0.125 mg/L for CRO, respectively. The mcr-1 and mcr-2 genes were not detected in Salmonella isolates. CONCLUSIONS: High-level and multi-resistance were not observed in our isolates. While resistance was generally low, the highest resistance rate was observed against colistin.http://www.sciencedirect.com/science/article/pii/S2213716524002431Salmonellacolistinantibiotic resistancemcr |
| spellingShingle | Rumeysa Bildik Betigül Öngen Resistance to colistin and various antibiotics in non-typhoidal Salmonella Journal of Global Antimicrobial Resistance Salmonella colistin antibiotic resistance mcr |
| title | Resistance to colistin and various antibiotics in non-typhoidal Salmonella |
| title_full | Resistance to colistin and various antibiotics in non-typhoidal Salmonella |
| title_fullStr | Resistance to colistin and various antibiotics in non-typhoidal Salmonella |
| title_full_unstemmed | Resistance to colistin and various antibiotics in non-typhoidal Salmonella |
| title_short | Resistance to colistin and various antibiotics in non-typhoidal Salmonella |
| title_sort | resistance to colistin and various antibiotics in non typhoidal salmonella |
| topic | Salmonella colistin antibiotic resistance mcr |
| url | http://www.sciencedirect.com/science/article/pii/S2213716524002431 |
| work_keys_str_mv | AT rumeysabildik resistancetocolistinandvariousantibioticsinnontyphoidalsalmonella AT betigulongen resistancetocolistinandvariousantibioticsinnontyphoidalsalmonella |