Conservation of in vitro shoots of Rhodiola sachalinensis by slow growth and its DNA content analysis
In order to establish conservation system of in vitro shoots of Rhodiola sachalinensis by slow growth, studies on conservation of in vitro shoots of two resources by slow growth under low temperature, week illumination and mannitol of different concentration were carried out, and DNA contents of tho...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2007-07-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2007.04.0373 |
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| author | LIU Jian-feng YAN Xiu-feng CHENG Yun-qing ZHOU Xiao-mei ZHANG Xiao-yan |
| author_facet | LIU Jian-feng YAN Xiu-feng CHENG Yun-qing ZHOU Xiao-mei ZHANG Xiao-yan |
| author_sort | LIU Jian-feng |
| collection | DOAJ |
| description | In order to establish conservation system of in vitro shoots of Rhodiola sachalinensis by slow growth, studies on conservation of in vitro shoots of two resources by slow growth under low temperature, week illumination and mannitol of different concentration were carried out, and DNA contents of those recovered plantlets were analyzed. The results indicated that more than 85% of in vitro shoots conserved on 1/2MS supplemented with 20 g·L<sup>-1</sup> sucrose, 7 g·L<sup>-1</sup> agar and 10 g·L<sup>-1</sup> mannitol maintained viability after 18 months under (6±1)℃ and 600 lx conditions. Both nuclear DNA contents maintained as normal. Thus, it was suggested these procedures would be effective and viable to conserve Rhodiola sachalinensis by slow growth as above. |
| format | Article |
| id | doaj-art-664c69a0f0e44963b30c3aaf095deee3 |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2007-07-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-664c69a0f0e44963b30c3aaf095deee32025-08-20T03:16:10ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552007-07-013337337810.3785/1008-9209.2007.04.037310089209Conservation of in vitro shoots of Rhodiola sachalinensis by slow growth and its DNA content analysisLIU Jian-fengYAN Xiu-fengCHENG Yun-qingZHOU Xiao-meiZHANG Xiao-yanIn order to establish conservation system of in vitro shoots of Rhodiola sachalinensis by slow growth, studies on conservation of in vitro shoots of two resources by slow growth under low temperature, week illumination and mannitol of different concentration were carried out, and DNA contents of those recovered plantlets were analyzed. The results indicated that more than 85% of in vitro shoots conserved on 1/2MS supplemented with 20 g·L<sup>-1</sup> sucrose, 7 g·L<sup>-1</sup> agar and 10 g·L<sup>-1</sup> mannitol maintained viability after 18 months under (6±1)℃ and 600 lx conditions. Both nuclear DNA contents maintained as normal. Thus, it was suggested these procedures would be effective and viable to conserve Rhodiola sachalinensis by slow growth as above.https://www.academax.com/doi/10.3785/1008-9209.2007.04.0373<italic>Rhodiola sachalinensis</italic>mannitolslow growth preservation |
| spellingShingle | LIU Jian-feng YAN Xiu-feng CHENG Yun-qing ZHOU Xiao-mei ZHANG Xiao-yan Conservation of in vitro shoots of Rhodiola sachalinensis by slow growth and its DNA content analysis 浙江大学学报. 农业与生命科学版 <italic>Rhodiola sachalinensis</italic> mannitol slow growth preservation |
| title | Conservation of in vitro shoots of Rhodiola sachalinensis by slow growth and its DNA content analysis |
| title_full | Conservation of in vitro shoots of Rhodiola sachalinensis by slow growth and its DNA content analysis |
| title_fullStr | Conservation of in vitro shoots of Rhodiola sachalinensis by slow growth and its DNA content analysis |
| title_full_unstemmed | Conservation of in vitro shoots of Rhodiola sachalinensis by slow growth and its DNA content analysis |
| title_short | Conservation of in vitro shoots of Rhodiola sachalinensis by slow growth and its DNA content analysis |
| title_sort | conservation of in vitro shoots of rhodiola sachalinensis by slow growth and its dna content analysis |
| topic | <italic>Rhodiola sachalinensis</italic> mannitol slow growth preservation |
| url | https://www.academax.com/doi/10.3785/1008-9209.2007.04.0373 |
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