Inhibitory effect of immune checkpoint cytotoxic t-lymphocyte- associated antigen-4 inhibitor combined with curcumin on tumor growth in mice with lung cancer
We aimed to investigate whether immune checkpoint cytotoxic T-lymphocyteassociated antigen-4 (CTLA-4) inhibitor plus curcumin can inhibit tumor growth in mice with lung cancer. A mouse model of lung cancer was established by injecting Lewis lung cancer cells. The mice were allocated to an anti-CTLA-...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2025-07-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=3205 |
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| _version_ | 1849761467603091456 |
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| author | Weiwei TANG Hailan WU Tangfeng LV |
| author_facet | Weiwei TANG Hailan WU Tangfeng LV |
| author_sort | Weiwei TANG |
| collection | DOAJ |
| description | We aimed to investigate whether immune checkpoint cytotoxic T-lymphocyteassociated
antigen-4 (CTLA-4) inhibitor plus curcumin can inhibit tumor growth in
mice with lung cancer. A mouse model of lung cancer was established by injecting
Lewis lung cancer cells. The mice were allocated to an anti-CTLA-4 group, a curcumin
group, an anti-CTLA-4+curcumin group, and a Model group in random (n=10). The
tumor inhibition rate and metastasis inhibition rate were calculated. Hematoxylin-eosin
staining was performed on tumor tissues for their pathological changes. The apoptosis
rate was measured by TUNEL assay. In comparison with the Model group, the tumor
volume, tumor mass, number of lung metastatic nodules, and the protein and relative
mRNA expressions of Bcl-2 in tumor tissues significantly decreased in anti-CTLA-4,
curcumin, and anti-CTLA-4+curcumin groups, while the tumor inhibition rate,
metastasis inhibition rate, apoptosis rate of tumor cells, and Caspase-3 and Bax contents
in tumor tissues increased (P<0.0001). CTLA-4 inhibitor plus curcumin can inhibit
tumor growth in mice with lung cancer, which may be associated with the promotion
of lung cancer cell apoptosis by regulating the Caspase-3/Bcl-2/Bax signaling pathway. |
| format | Article |
| id | doaj-art-664732dbc19a4f17b88ddba30002a862 |
| institution | DOAJ |
| issn | 1309-2251 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj-art-664732dbc19a4f17b88ddba30002a8622025-08-20T03:06:01ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512025-07-0131445946610.9775/kvfd.2025.336183205Inhibitory effect of immune checkpoint cytotoxic t-lymphocyte- associated antigen-4 inhibitor combined with curcumin on tumor growth in mice with lung cancerWeiwei TANG0Hailan WU1Tangfeng LV2Nanjing University of Chinese Medicine, Nanjing 210029, Jiangsu Province, CHINAJinling Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210002, Jiangsu Province, CHINAJinling Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210002, Jiangsu Province, CHINAWe aimed to investigate whether immune checkpoint cytotoxic T-lymphocyteassociated antigen-4 (CTLA-4) inhibitor plus curcumin can inhibit tumor growth in mice with lung cancer. A mouse model of lung cancer was established by injecting Lewis lung cancer cells. The mice were allocated to an anti-CTLA-4 group, a curcumin group, an anti-CTLA-4+curcumin group, and a Model group in random (n=10). The tumor inhibition rate and metastasis inhibition rate were calculated. Hematoxylin-eosin staining was performed on tumor tissues for their pathological changes. The apoptosis rate was measured by TUNEL assay. In comparison with the Model group, the tumor volume, tumor mass, number of lung metastatic nodules, and the protein and relative mRNA expressions of Bcl-2 in tumor tissues significantly decreased in anti-CTLA-4, curcumin, and anti-CTLA-4+curcumin groups, while the tumor inhibition rate, metastasis inhibition rate, apoptosis rate of tumor cells, and Caspase-3 and Bax contents in tumor tissues increased (P<0.0001). CTLA-4 inhibitor plus curcumin can inhibit tumor growth in mice with lung cancer, which may be associated with the promotion of lung cancer cell apoptosis by regulating the Caspase-3/Bcl-2/Bax signaling pathway.https://vetdergikafkas.org/pdf.php?id=3205curcumintumor growthinhibitorlung cancer |
| spellingShingle | Weiwei TANG Hailan WU Tangfeng LV Inhibitory effect of immune checkpoint cytotoxic t-lymphocyte- associated antigen-4 inhibitor combined with curcumin on tumor growth in mice with lung cancer Kafkas Universitesi Veteriner Fakültesi Dergisi curcumin tumor growth inhibitor lung cancer |
| title | Inhibitory effect of immune checkpoint cytotoxic t-lymphocyte- associated antigen-4 inhibitor combined with curcumin on tumor growth in mice with lung cancer |
| title_full | Inhibitory effect of immune checkpoint cytotoxic t-lymphocyte- associated antigen-4 inhibitor combined with curcumin on tumor growth in mice with lung cancer |
| title_fullStr | Inhibitory effect of immune checkpoint cytotoxic t-lymphocyte- associated antigen-4 inhibitor combined with curcumin on tumor growth in mice with lung cancer |
| title_full_unstemmed | Inhibitory effect of immune checkpoint cytotoxic t-lymphocyte- associated antigen-4 inhibitor combined with curcumin on tumor growth in mice with lung cancer |
| title_short | Inhibitory effect of immune checkpoint cytotoxic t-lymphocyte- associated antigen-4 inhibitor combined with curcumin on tumor growth in mice with lung cancer |
| title_sort | inhibitory effect of immune checkpoint cytotoxic t lymphocyte associated antigen 4 inhibitor combined with curcumin on tumor growth in mice with lung cancer |
| topic | curcumin tumor growth inhibitor lung cancer |
| url | https://vetdergikafkas.org/pdf.php?id=3205 |
| work_keys_str_mv | AT weiweitang inhibitoryeffectofimmunecheckpointcytotoxictlymphocyteassociatedantigen4inhibitorcombinedwithcurcuminontumorgrowthinmicewithlungcancer AT hailanwu inhibitoryeffectofimmunecheckpointcytotoxictlymphocyteassociatedantigen4inhibitorcombinedwithcurcuminontumorgrowthinmicewithlungcancer AT tangfenglv inhibitoryeffectofimmunecheckpointcytotoxictlymphocyteassociatedantigen4inhibitorcombinedwithcurcuminontumorgrowthinmicewithlungcancer |