Serum calcium and its relationship with immunoglobulin G (IgG) levels in prepartum buffaloes from the Venezuelan humid tropics
The immune system partially recognizes specific antigens due to the role of calcium (Ca2+) as an intracellular second messenger that regulates the humoral immune response. To consider a physiological association between serum Ca2+ levels and its modulatory effect on immunoglobulin G (IgG) synthesi...
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Universidad del Zulia
2023-11-01
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| author | Juan Pablo Uzcátegui-Varela Anacelmira Urbina Andueza Janeth Caamaño Carrero |
| author_facet | Juan Pablo Uzcátegui-Varela Anacelmira Urbina Andueza Janeth Caamaño Carrero |
| author_sort | Juan Pablo Uzcátegui-Varela |
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The immune system partially recognizes specific antigens due to the role of calcium (Ca2+) as an intracellular second messenger that regulates the humoral immune response. To consider a physiological association between serum Ca2+ levels and its modulatory effect on immunoglobulin G (IgG) synthesis before calving in dairy buffaloes (Bubalus bubalis) in southern Lake Maracaibo, Venezuela, twenty clinically healthy Murrah x Mediterranean crossbred female buffaloes with excellent body condition were considered for this study. Ten were young females of 33.6±14.15 months, with nine months of gestation and 432±44.27 kg body weight. The other ten were adult buffaloes of 563±53.6 kg body weight at nine months of gestation and reproductive history of four calvings. The sampling consisted of collecting 6 milliliters (mL) of blood by jugular venipuncture in tubes without anticoagulant; then, the serum was separated by centrifugation at 3,300 r.p.m. for 20 minutes at 25 °C using a BOECO®-Germany centrifuge. Next, 1 mL of serum was taken to determine the serological parameters under study. Ca2+ was found in the blood through the Arsenazo III method using a Selectra ProsS ELITech®- Netherlands Analyzer and, to quantify the levels of IgG; the immunoturbidimetry technique was used with the Cobas c311 Roche® Analyzer®-USA. The data were subjected to the Kruskal-Wallis nonparametric test and Spearman correlation analysis. According to the results obtained, primiparous B. bubalis females close to parturition presented serum Ca2+ levels (8.03±0.25 mg/dL) similar to multiparous buffaloes (8.07±0.7 mg/dL) adjusted to the reference range (6.05-11.98 mg/dL) for water buffaloes. In contrast, IgG in young females was higher (761.33±21.81 mg/dL) concerning adult buffaloes (735. 83±17.39 mg/dL) with no statistically significant differences (p>0.05) between groups for both variables; however, the correlation test indicated that, in first calving buffaloes, the circulating Ca2+ level is negatively associated (r=-0.56) with IgG concentration, a physiological response that was statistically different (p<0.05) concerning multiparous buffaloes (r=0.83). There is evidence of an association between serum calcium concentration and IgG levels that depends on the number of calvings, possibly because different metabolic pathways for Ca 2+ exchange determine the immune response among the categories of buffaloes included in the study. Thus, the functional significance of Ca2+ signaling for the prepartum B. bubalis immune system is just beginning to emerge.
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| format | Article |
| id | doaj-art-662c834725174d18aa139866f084b80c |
| institution | Kabale University |
| issn | 0798-2259 2521-9715 |
| language | English |
| publishDate | 2023-11-01 |
| publisher | Universidad del Zulia |
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| spelling | doaj-art-662c834725174d18aa139866f084b80c2025-01-29T15:36:56ZengUniversidad del ZuliaRevista Científica0798-22592521-97152023-11-0133Suplemento10.52973/rcfcv-wbc077Serum calcium and its relationship with immunoglobulin G (IgG) levels in prepartum buffaloes from the Venezuelan humid tropicsJuan Pablo Uzcátegui-Varela0Anacelmira Urbina Andueza1Janeth Caamaño Carrero2Animal Science and Tropical Plants Research Group. Universidad Nacional Experimental Sur del Lago “Jesús María Semprum” (UNESUR) Núcleo La Victoria, Mérida, Venezuela.Institute of Agricultural Research, Faculty of Forestry and Environmental Sciences, Universidad de Los Andes, Mérida, Venezuela.Institute of Agricultural Research, Faculty of Forestry and Environmental Sciences, Universidad de Los Andes, Mérida, Venezuela. The immune system partially recognizes specific antigens due to the role of calcium (Ca2+) as an intracellular second messenger that regulates the humoral immune response. To consider a physiological association between serum Ca2+ levels and its modulatory effect on immunoglobulin G (IgG) synthesis before calving in dairy buffaloes (Bubalus bubalis) in southern Lake Maracaibo, Venezuela, twenty clinically healthy Murrah x Mediterranean crossbred female buffaloes with excellent body condition were considered for this study. Ten were young females of 33.6±14.15 months, with nine months of gestation and 432±44.27 kg body weight. The other ten were adult buffaloes of 563±53.6 kg body weight at nine months of gestation and reproductive history of four calvings. The sampling consisted of collecting 6 milliliters (mL) of blood by jugular venipuncture in tubes without anticoagulant; then, the serum was separated by centrifugation at 3,300 r.p.m. for 20 minutes at 25 °C using a BOECO®-Germany centrifuge. Next, 1 mL of serum was taken to determine the serological parameters under study. Ca2+ was found in the blood through the Arsenazo III method using a Selectra ProsS ELITech®- Netherlands Analyzer and, to quantify the levels of IgG; the immunoturbidimetry technique was used with the Cobas c311 Roche® Analyzer®-USA. The data were subjected to the Kruskal-Wallis nonparametric test and Spearman correlation analysis. According to the results obtained, primiparous B. bubalis females close to parturition presented serum Ca2+ levels (8.03±0.25 mg/dL) similar to multiparous buffaloes (8.07±0.7 mg/dL) adjusted to the reference range (6.05-11.98 mg/dL) for water buffaloes. In contrast, IgG in young females was higher (761.33±21.81 mg/dL) concerning adult buffaloes (735. 83±17.39 mg/dL) with no statistically significant differences (p>0.05) between groups for both variables; however, the correlation test indicated that, in first calving buffaloes, the circulating Ca2+ level is negatively associated (r=-0.56) with IgG concentration, a physiological response that was statistically different (p<0.05) concerning multiparous buffaloes (r=0.83). There is evidence of an association between serum calcium concentration and IgG levels that depends on the number of calvings, possibly because different metabolic pathways for Ca 2+ exchange determine the immune response among the categories of buffaloes included in the study. Thus, the functional significance of Ca2+ signaling for the prepartum B. bubalis immune system is just beginning to emerge. https://www.produccioncientificaluz.org/index.php/cientifica/article/view/43375Bubalus bubalisIgGB lymphocyteimmune responsecell signaling |
| spellingShingle | Juan Pablo Uzcátegui-Varela Anacelmira Urbina Andueza Janeth Caamaño Carrero Serum calcium and its relationship with immunoglobulin G (IgG) levels in prepartum buffaloes from the Venezuelan humid tropics Revista Científica Bubalus bubalis IgG B lymphocyte immune response cell signaling |
| title | Serum calcium and its relationship with immunoglobulin G (IgG) levels in prepartum buffaloes from the Venezuelan humid tropics |
| title_full | Serum calcium and its relationship with immunoglobulin G (IgG) levels in prepartum buffaloes from the Venezuelan humid tropics |
| title_fullStr | Serum calcium and its relationship with immunoglobulin G (IgG) levels in prepartum buffaloes from the Venezuelan humid tropics |
| title_full_unstemmed | Serum calcium and its relationship with immunoglobulin G (IgG) levels in prepartum buffaloes from the Venezuelan humid tropics |
| title_short | Serum calcium and its relationship with immunoglobulin G (IgG) levels in prepartum buffaloes from the Venezuelan humid tropics |
| title_sort | serum calcium and its relationship with immunoglobulin g igg levels in prepartum buffaloes from the venezuelan humid tropics |
| topic | Bubalus bubalis IgG B lymphocyte immune response cell signaling |
| url | https://www.produccioncientificaluz.org/index.php/cientifica/article/view/43375 |
| work_keys_str_mv | AT juanpablouzcateguivarela serumcalciumanditsrelationshipwithimmunoglobulingigglevelsinprepartumbuffaloesfromthevenezuelanhumidtropics AT anacelmiraurbinaandueza serumcalciumanditsrelationshipwithimmunoglobulingigglevelsinprepartumbuffaloesfromthevenezuelanhumidtropics AT janethcaamanocarrero serumcalciumanditsrelationshipwithimmunoglobulingigglevelsinprepartumbuffaloesfromthevenezuelanhumidtropics |