Mannose-B from Codonopsis pilosula modulates LAMB1 expression to enhance trophoblast function and alleviate subchorionic hematoma
Abstract This study investigates the active component Mannose-B from Codonopsis pilosula and its effect on human trophoblast cell function, particularly focusing on the regulation of Laminin Subunit Beta 1 (LAMB1) expression and its implications in subchorionic hematoma (SCH). Key genes involved in...
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Nature Portfolio
2025-08-01
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| Online Access: | https://doi.org/10.1038/s41598-025-13918-z |
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| author | Qian Sun Yuan Gao Kadirya Asan Jing Ji Jinjin Xu Xiaoyan Wu Jie Ren Wen Feng Mengwei Song Sen Wang Boyu Zhang Ying Zhou Conghui Han Shun Liu Wen Yang |
| author_facet | Qian Sun Yuan Gao Kadirya Asan Jing Ji Jinjin Xu Xiaoyan Wu Jie Ren Wen Feng Mengwei Song Sen Wang Boyu Zhang Ying Zhou Conghui Han Shun Liu Wen Yang |
| author_sort | Qian Sun |
| collection | DOAJ |
| description | Abstract This study investigates the active component Mannose-B from Codonopsis pilosula and its effect on human trophoblast cell function, particularly focusing on the regulation of Laminin Subunit Beta 1 (LAMB1) expression and its implications in subchorionic hematoma (SCH). Key genes involved in SCH pathology were identified through RNA sequencing and bioinformatics analysis. Network pharmacology was utilized to screen active components in Codonopsis pilosula and their critical targets. In vitro, HTR-8/Svneo cells were used to assess proliferation, migration, and invasion through CCK8, Transwell, and cell migration assays. A SCH rat model was established to evaluate changes in coagulation parameters, litter size, fetal viability, and fetal and placental weights. In vivo validation of Mannose-B’s effects on LAMB1 expression and SCH pathology was performed using RT-qPCR and Western Blot. Network pharmacology and molecular docking identified Mannose-B as a potentially active compound in Codonopsis pilosula, with LAMB1 as a significant predicted target. In vitro experiments suggested that Mannose-B may promote HTR-8/Svneo cell proliferation, migration, and invasion by downregulating LAMB1 expression. In vivo experiments showed a reduction in placental LAMB1 expression and partial improvement in SCH-related pathological features. Mannose-B from Codonopsis pilosula may alleviate SCH progression by modulating LAMB1 expression and enhancing trophoblast cell function. However, these findings remain preliminary and require further investigation to confirm the underlying mechanisms and clinical relevance. |
| format | Article |
| id | doaj-art-6627b9556a264090aa8ea37b76e60a98 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-6627b9556a264090aa8ea37b76e60a982025-08-20T04:02:45ZengNature PortfolioScientific Reports2045-23222025-08-0115111610.1038/s41598-025-13918-zMannose-B from Codonopsis pilosula modulates LAMB1 expression to enhance trophoblast function and alleviate subchorionic hematomaQian Sun0Yuan Gao1Kadirya Asan2Jing Ji3Jinjin Xu4Xiaoyan Wu5Jie Ren6Wen Feng7Mengwei Song8Sen Wang9Boyu Zhang10Ying Zhou11Conghui Han12Shun Liu13Wen Yang14Department of Gynecology, The first people’s hospital of LianyungangDepartment of Gynecology, The first people’s hospital of LianyungangJiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, College of Pharmacy, Jiangsu Ocean UniversityJiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, College of Pharmacy, Jiangsu Ocean UniversityDepartment of Gynecology, The first people’s hospital of LianyungangDepartment of Gynecology, The first people’s hospital of LianyungangDepartment of Gynecology, The first people’s hospital of LianyungangDepartment of Gynecology, The first people’s hospital of LianyungangJiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, College of Pharmacy, Jiangsu Ocean UniversityJiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, College of Pharmacy, Jiangsu Ocean UniversityJiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, College of Pharmacy, Jiangsu Ocean UniversityJiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, College of Pharmacy, Jiangsu Ocean UniversityDepartment of Urology, Xuzhou Clinical School of Xuzhou Medical UniversityKangda College of Nanjing Medical UniversityDepartment of Gynecology, The first people’s hospital of LianyungangAbstract This study investigates the active component Mannose-B from Codonopsis pilosula and its effect on human trophoblast cell function, particularly focusing on the regulation of Laminin Subunit Beta 1 (LAMB1) expression and its implications in subchorionic hematoma (SCH). Key genes involved in SCH pathology were identified through RNA sequencing and bioinformatics analysis. Network pharmacology was utilized to screen active components in Codonopsis pilosula and their critical targets. In vitro, HTR-8/Svneo cells were used to assess proliferation, migration, and invasion through CCK8, Transwell, and cell migration assays. A SCH rat model was established to evaluate changes in coagulation parameters, litter size, fetal viability, and fetal and placental weights. In vivo validation of Mannose-B’s effects on LAMB1 expression and SCH pathology was performed using RT-qPCR and Western Blot. Network pharmacology and molecular docking identified Mannose-B as a potentially active compound in Codonopsis pilosula, with LAMB1 as a significant predicted target. In vitro experiments suggested that Mannose-B may promote HTR-8/Svneo cell proliferation, migration, and invasion by downregulating LAMB1 expression. In vivo experiments showed a reduction in placental LAMB1 expression and partial improvement in SCH-related pathological features. Mannose-B from Codonopsis pilosula may alleviate SCH progression by modulating LAMB1 expression and enhancing trophoblast cell function. However, these findings remain preliminary and require further investigation to confirm the underlying mechanisms and clinical relevance.https://doi.org/10.1038/s41598-025-13918-zSubchorionic hematomaCodonopsis pilosulaMannose-BLaminin subunit beta 1Trophoblast cellsNetwork pharmacology |
| spellingShingle | Qian Sun Yuan Gao Kadirya Asan Jing Ji Jinjin Xu Xiaoyan Wu Jie Ren Wen Feng Mengwei Song Sen Wang Boyu Zhang Ying Zhou Conghui Han Shun Liu Wen Yang Mannose-B from Codonopsis pilosula modulates LAMB1 expression to enhance trophoblast function and alleviate subchorionic hematoma Scientific Reports Subchorionic hematoma Codonopsis pilosula Mannose-B Laminin subunit beta 1 Trophoblast cells Network pharmacology |
| title | Mannose-B from Codonopsis pilosula modulates LAMB1 expression to enhance trophoblast function and alleviate subchorionic hematoma |
| title_full | Mannose-B from Codonopsis pilosula modulates LAMB1 expression to enhance trophoblast function and alleviate subchorionic hematoma |
| title_fullStr | Mannose-B from Codonopsis pilosula modulates LAMB1 expression to enhance trophoblast function and alleviate subchorionic hematoma |
| title_full_unstemmed | Mannose-B from Codonopsis pilosula modulates LAMB1 expression to enhance trophoblast function and alleviate subchorionic hematoma |
| title_short | Mannose-B from Codonopsis pilosula modulates LAMB1 expression to enhance trophoblast function and alleviate subchorionic hematoma |
| title_sort | mannose b from codonopsis pilosula modulates lamb1 expression to enhance trophoblast function and alleviate subchorionic hematoma |
| topic | Subchorionic hematoma Codonopsis pilosula Mannose-B Laminin subunit beta 1 Trophoblast cells Network pharmacology |
| url | https://doi.org/10.1038/s41598-025-13918-z |
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