Therapeutic Evaluation <i>Punica granatum</i> Peel Powder for the Ailment of Inflammatory Bowel Disorder in NCM460 Cell Line and in Albino Rats
<b>Background:</b> Ulcerative colitis (UC) is a chronic inflammatory condition associated with the colon and rectum, often predisposing individuals to inflammatory bowel disease-related colorectal cancer (IBD-CRC). Current therapeutic options for UC, including corticosteroids and immunos...
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| author | Parikshit Roychowdhury Gyanendra Kumar Prajapati Rupesh Singh Prasanna Gurunath Ramesh C Gowthamarajan Kuppuswamy Anindita De |
| author_facet | Parikshit Roychowdhury Gyanendra Kumar Prajapati Rupesh Singh Prasanna Gurunath Ramesh C Gowthamarajan Kuppuswamy Anindita De |
| author_sort | Parikshit Roychowdhury |
| collection | DOAJ |
| description | <b>Background:</b> Ulcerative colitis (UC) is a chronic inflammatory condition associated with the colon and rectum, often predisposing individuals to inflammatory bowel disease-related colorectal cancer (IBD-CRC). Current therapeutic options for UC, including corticosteroids and immunosuppressive drugs, pose significant side effects. <i>Punica granatum</i> peel powder (PPPG), a traditional herbal remedy in Ayurveda medicine for colitis, exhibits promising therapeutic effects with a favorable safety profile. <b>Objectives:</b> This study aims to explore the therapeutic potential and mechanism of action of a modified PPPG formulation in UC treatment. <b>Methods:</b> Using NCM460 cells and an acetic acid-induced UC murine model, the efficacy of modified PPPG was evaluated. <b>Results:</b> Therapy with modified PPPG significantly improved UC-associated symptoms, such as improvements in body weight, colon length, and disease activity index, as validated by histological examination. Transcriptomic sequencing identified downregulation of the IL-6/STAT3 signaling pathway and reduced inflammatory markers like p-NF-κB, IL-1β, and NLRP3 on PPPG therapy. <b>Conclusions:</b> These findings suggest that modified PPPG holds promise as a novel therapeutic strategy for UC intervention, targeting key inflammatory pathways implicated in UC pathogenesis and potentially mitigating the risk of IBD-CRC. |
| format | Article |
| id | doaj-art-661dd2ea4b1f4614a6943bc8773c8474 |
| institution | DOAJ |
| issn | 1999-4923 |
| language | English |
| publishDate | 2025-06-01 |
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| spelling | doaj-art-661dd2ea4b1f4614a6943bc8773c84742025-08-20T02:47:10ZengMDPI AGPharmaceutics1999-49232025-06-0117784310.3390/pharmaceutics17070843Therapeutic Evaluation <i>Punica granatum</i> Peel Powder for the Ailment of Inflammatory Bowel Disorder in NCM460 Cell Line and in Albino RatsParikshit Roychowdhury0Gyanendra Kumar Prajapati1Rupesh Singh2Prasanna Gurunath3Ramesh C4Gowthamarajan Kuppuswamy5Anindita De6Department of Pharmaceutics, JSS College of Pharmacy, JSS Academy of Higher Education and Research, Ooty 643001, Tamil Nadu, IndiaDepartment of Pharmacology, KLE College of Pharmacy, KLE Academy of Higher Education and Research, Belagavi 590010, Karnataka, IndiaDepartment of Pharmacology, St. John’s Pharmacy College, Vijayanagar, Bengaluru 560104, Karnataka, IndiaDepartment of Pharmacology, SBD Institute of Pharmacy, Bengaluru 560019, Karnataka, IndiaDepartment of Pharmacology, East West College of Pharmacy, Bengaluru 560091, Karnataka, IndiaDepartment of Pharmaceutics, JSS College of Pharmacy, JSS Academy of Higher Education and Research, Ooty 643001, Tamil Nadu, IndiaDepartment of Pharmaceutics, College of Pharmacy, JSS University, Noida 201301, Uttar Pradesh, India<b>Background:</b> Ulcerative colitis (UC) is a chronic inflammatory condition associated with the colon and rectum, often predisposing individuals to inflammatory bowel disease-related colorectal cancer (IBD-CRC). Current therapeutic options for UC, including corticosteroids and immunosuppressive drugs, pose significant side effects. <i>Punica granatum</i> peel powder (PPPG), a traditional herbal remedy in Ayurveda medicine for colitis, exhibits promising therapeutic effects with a favorable safety profile. <b>Objectives:</b> This study aims to explore the therapeutic potential and mechanism of action of a modified PPPG formulation in UC treatment. <b>Methods:</b> Using NCM460 cells and an acetic acid-induced UC murine model, the efficacy of modified PPPG was evaluated. <b>Results:</b> Therapy with modified PPPG significantly improved UC-associated symptoms, such as improvements in body weight, colon length, and disease activity index, as validated by histological examination. Transcriptomic sequencing identified downregulation of the IL-6/STAT3 signaling pathway and reduced inflammatory markers like p-NF-κB, IL-1β, and NLRP3 on PPPG therapy. <b>Conclusions:</b> These findings suggest that modified PPPG holds promise as a novel therapeutic strategy for UC intervention, targeting key inflammatory pathways implicated in UC pathogenesis and potentially mitigating the risk of IBD-CRC.https://www.mdpi.com/1999-4923/17/7/843<i>Punica granatum</i>inflammatory bowel diseasepre-clinical studyenterocolitischemical induced colitis |
| spellingShingle | Parikshit Roychowdhury Gyanendra Kumar Prajapati Rupesh Singh Prasanna Gurunath Ramesh C Gowthamarajan Kuppuswamy Anindita De Therapeutic Evaluation <i>Punica granatum</i> Peel Powder for the Ailment of Inflammatory Bowel Disorder in NCM460 Cell Line and in Albino Rats Pharmaceutics <i>Punica granatum</i> inflammatory bowel disease pre-clinical study enterocolitis chemical induced colitis |
| title | Therapeutic Evaluation <i>Punica granatum</i> Peel Powder for the Ailment of Inflammatory Bowel Disorder in NCM460 Cell Line and in Albino Rats |
| title_full | Therapeutic Evaluation <i>Punica granatum</i> Peel Powder for the Ailment of Inflammatory Bowel Disorder in NCM460 Cell Line and in Albino Rats |
| title_fullStr | Therapeutic Evaluation <i>Punica granatum</i> Peel Powder for the Ailment of Inflammatory Bowel Disorder in NCM460 Cell Line and in Albino Rats |
| title_full_unstemmed | Therapeutic Evaluation <i>Punica granatum</i> Peel Powder for the Ailment of Inflammatory Bowel Disorder in NCM460 Cell Line and in Albino Rats |
| title_short | Therapeutic Evaluation <i>Punica granatum</i> Peel Powder for the Ailment of Inflammatory Bowel Disorder in NCM460 Cell Line and in Albino Rats |
| title_sort | therapeutic evaluation i punica granatum i peel powder for the ailment of inflammatory bowel disorder in ncm460 cell line and in albino rats |
| topic | <i>Punica granatum</i> inflammatory bowel disease pre-clinical study enterocolitis chemical induced colitis |
| url | https://www.mdpi.com/1999-4923/17/7/843 |
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