Microbial fertilizers modulate tobacco growth and development through reshaping soil microbiome and metabolome

ABSTRACT To elucidate the mechanisms of microbial fertilizers in enhancing tobacco growth and quality, this greenhouse-based pot experiment conducted over 40 days post-transplanting employed integrated microbiomics and metabolomics approaches to conduct a comparative analysis among conventional chem...

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Main Authors: Xiaoyu Wang, Mingming Sun, Lei Tian, Mingfeng Yang, Qiang Gao, LIli Wang, Honghao Yan, Long Yang, Xin Hou, Peng Liu, Li Zhang
Format: Article
Language:English
Published: American Society for Microbiology 2025-07-01
Series:Microbiology Spectrum
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Online Access:https://journals.asm.org/doi/10.1128/spectrum.02605-24
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author Xiaoyu Wang
Mingming Sun
Lei Tian
Mingfeng Yang
Qiang Gao
LIli Wang
Honghao Yan
Long Yang
Xin Hou
Peng Liu
Li Zhang
author_facet Xiaoyu Wang
Mingming Sun
Lei Tian
Mingfeng Yang
Qiang Gao
LIli Wang
Honghao Yan
Long Yang
Xin Hou
Peng Liu
Li Zhang
author_sort Xiaoyu Wang
collection DOAJ
description ABSTRACT To elucidate the mechanisms of microbial fertilizers in enhancing tobacco growth and quality, this greenhouse-based pot experiment conducted over 40 days post-transplanting employed integrated microbiomics and metabolomics approaches to conduct a comparative analysis among conventional chemical, organic, and microbial fertilizers. Plant agronomic traits were systematically assessed at 20, 30, and 40 days post-transplanting, while soil physicochemical parameters were analyzed at the experimental terminus (40 days). The findings underscored the remarkable potential of microbial fertilizers in augmenting soil’s quick-release nutrient pool and bolstering soil enzymatic activity, surpassing both chemical and organic counterparts. The application of microbial fertilizers accelerated tobacco growth and development, and significantly elevated agronomic indices, including plant stature, stem girth, leaf extension, and the abundance of aromatic precursors, thereby facilitating a marked improvement in tobacco leaf quality. Furthermore, the microbial community composition underwent pronounced alterations subsequent to the application of microbial fertilizers, with the emergence of pivotal microorganisms such as Rhodanobacter and Pseudolabrys within the treatment group. These microorganisms emerged as vital players in nutrient cycling processes, fostered plant growth, and mitigated the incidence of plant diseases. Microbial fertilizers demonstrated a significantly superior capacity to stimulate metabolic vigor in tobacco plants compared to other treatments, concomitant with a substantial enrichment of several metabolites, such as 3-methylindole. These data collectively imply that microbial fertilizers represent a more efficacious means of ameliorating soil physicochemical attributes, thereby fostering superior tobacco growth, development, and quality enhancement.IMPORTANCEIn recent years, there has been a surge in research examining the impacts of various fertilizers on the microbial composition of tobacco rhizosphere soils, and numerous studies have consecutively reported the growth-promoting mechanisms of diverse fertilizers on tobacco plants. However, despite these advancements, the existing body of literature remains inadequate in conclusively demonstrating the superiority of microbial fertilizers over traditional organic and inorganic fertilizers in tobacco cultivation. Consequently, our research aims to demonstrate the superiority of microbial fertilizers in enhancing plant growth by utilizing a comprehensive approach that integrates microbiomics and metabolomics techniques.
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spelling doaj-art-661405ffa21e4eb993881770e67b05ab2025-08-20T03:28:22ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972025-07-0113710.1128/spectrum.02605-24Microbial fertilizers modulate tobacco growth and development through reshaping soil microbiome and metabolomeXiaoyu Wang0Mingming Sun1Lei Tian2Mingfeng Yang3Qiang Gao4LIli Wang5Honghao Yan6Long Yang7Xin Hou8Peng Liu9Li Zhang10College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong, ChinaChina Tobacco Shandong Industrial Co., Ltd., Qing’dao, ChinaShandong Linyi Tobacco Co., Ltd., Linyi, ChinaChina Tobacco Shandong Industrial Co., Ltd., Qing’dao, ChinaChina Tobacco Shandong Industrial Co., Ltd., Qing’dao, ChinaShandong Linyi Tobacco Co., Ltd., Linyi, ChinaCollege of Plant Protection, Shandong Agricultural University, Tai'an, Shandong, ChinaCollege of Plant Protection, Shandong Agricultural University, Tai'an, Shandong, ChinaCollege of Plant Protection, Shandong Agricultural University, Tai'an, Shandong, ChinaCollege of Plant Protection, Shandong Agricultural University, Tai'an, Shandong, ChinaCollege of Plant Protection, Shandong Agricultural University, Tai'an, Shandong, ChinaABSTRACT To elucidate the mechanisms of microbial fertilizers in enhancing tobacco growth and quality, this greenhouse-based pot experiment conducted over 40 days post-transplanting employed integrated microbiomics and metabolomics approaches to conduct a comparative analysis among conventional chemical, organic, and microbial fertilizers. Plant agronomic traits were systematically assessed at 20, 30, and 40 days post-transplanting, while soil physicochemical parameters were analyzed at the experimental terminus (40 days). The findings underscored the remarkable potential of microbial fertilizers in augmenting soil’s quick-release nutrient pool and bolstering soil enzymatic activity, surpassing both chemical and organic counterparts. The application of microbial fertilizers accelerated tobacco growth and development, and significantly elevated agronomic indices, including plant stature, stem girth, leaf extension, and the abundance of aromatic precursors, thereby facilitating a marked improvement in tobacco leaf quality. Furthermore, the microbial community composition underwent pronounced alterations subsequent to the application of microbial fertilizers, with the emergence of pivotal microorganisms such as Rhodanobacter and Pseudolabrys within the treatment group. These microorganisms emerged as vital players in nutrient cycling processes, fostered plant growth, and mitigated the incidence of plant diseases. Microbial fertilizers demonstrated a significantly superior capacity to stimulate metabolic vigor in tobacco plants compared to other treatments, concomitant with a substantial enrichment of several metabolites, such as 3-methylindole. These data collectively imply that microbial fertilizers represent a more efficacious means of ameliorating soil physicochemical attributes, thereby fostering superior tobacco growth, development, and quality enhancement.IMPORTANCEIn recent years, there has been a surge in research examining the impacts of various fertilizers on the microbial composition of tobacco rhizosphere soils, and numerous studies have consecutively reported the growth-promoting mechanisms of diverse fertilizers on tobacco plants. However, despite these advancements, the existing body of literature remains inadequate in conclusively demonstrating the superiority of microbial fertilizers over traditional organic and inorganic fertilizers in tobacco cultivation. Consequently, our research aims to demonstrate the superiority of microbial fertilizers in enhancing plant growth by utilizing a comprehensive approach that integrates microbiomics and metabolomics techniques.https://journals.asm.org/doi/10.1128/spectrum.02605-24tobaccomulti-omicsmetabolismsoil microorganismsoil propertiesmicrobial fertilizers
spellingShingle Xiaoyu Wang
Mingming Sun
Lei Tian
Mingfeng Yang
Qiang Gao
LIli Wang
Honghao Yan
Long Yang
Xin Hou
Peng Liu
Li Zhang
Microbial fertilizers modulate tobacco growth and development through reshaping soil microbiome and metabolome
Microbiology Spectrum
tobacco
multi-omics
metabolism
soil microorganism
soil properties
microbial fertilizers
title Microbial fertilizers modulate tobacco growth and development through reshaping soil microbiome and metabolome
title_full Microbial fertilizers modulate tobacco growth and development through reshaping soil microbiome and metabolome
title_fullStr Microbial fertilizers modulate tobacco growth and development through reshaping soil microbiome and metabolome
title_full_unstemmed Microbial fertilizers modulate tobacco growth and development through reshaping soil microbiome and metabolome
title_short Microbial fertilizers modulate tobacco growth and development through reshaping soil microbiome and metabolome
title_sort microbial fertilizers modulate tobacco growth and development through reshaping soil microbiome and metabolome
topic tobacco
multi-omics
metabolism
soil microorganism
soil properties
microbial fertilizers
url https://journals.asm.org/doi/10.1128/spectrum.02605-24
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