Removing limitations surrounding terpenoid biosynthesis by biotechnological techniques in Ferula sp.: A review
Ferula sp. has achieved widespread fame as a producer of specialized terpenoids used as raw materials in fragrances, food, cosmetics, and pharmaceutical industries. Since ancient times, Ferula species has been utilized to treat various health issues, such as asthma, toothache, inflammation, cancer,...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | Current Plant Biology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214662825000234 |
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| author | Zahra Aghaali Jun-Li Yang Mohammad Reza Naghavi Meisam Zargar |
| author_facet | Zahra Aghaali Jun-Li Yang Mohammad Reza Naghavi Meisam Zargar |
| author_sort | Zahra Aghaali |
| collection | DOAJ |
| description | Ferula sp. has achieved widespread fame as a producer of specialized terpenoids used as raw materials in fragrances, food, cosmetics, and pharmaceutical industries. Since ancient times, Ferula species has been utilized to treat various health issues, such as asthma, toothache, inflammation, cancer, and digestive disorders. Besides, a growing body of research proves the healing efficacy of Ferula plants in treating modern diseases, such as multiple sclerosis (MS), HIV, and COVID19. The major challenge surrounding the commercialization of Ferula-derived terpenoids is their low quantity in Ferula plants. This necessitates the exploitation of approaches to circumvent this barrier and to enhance their production level to meet the continuous demands of industries for Ferula terpenoids. Recently, via functional genomics, omics technologies, and high-throughput analytical techniques, our understanding about terpenoid biosynthesis and regulation has been deepened, paving the way for the overproduction of target terpenoids. This review examines the potential of hairy root culture, CRISPR/Cas-mediated genome editing, and metabolic engineering, including gene overexpression and enzyme engineering, for enhancing Ferula capacity tailored to industrial and medicinal needs. The strategies present here, except hairy root culture, have never been proposed or applied in Ferula species. In its ultimate form, the proposed strategies are expected to reach large-scale terpenoid production. |
| format | Article |
| id | doaj-art-6610a57d3c6847bda25522fa1a0b48ec |
| institution | Kabale University |
| issn | 2214-6628 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Plant Biology |
| spelling | doaj-art-6610a57d3c6847bda25522fa1a0b48ec2025-02-07T04:47:37ZengElsevierCurrent Plant Biology2214-66282025-03-0141100455Removing limitations surrounding terpenoid biosynthesis by biotechnological techniques in Ferula sp.: A reviewZahra Aghaali0Jun-Li Yang1Mohammad Reza Naghavi2Meisam Zargar3Division of Plant Biotechnology, Department of Agronomy and Plant Breeding, College of Agricultural and Natural Resources, University of Tehran, Karaj, Iran; Corresponding author.CAS Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, University of Chinese Academy of Sciences (CAS), Lanzhou 730000, PR ChinaDivision of Plant Biotechnology, Department of Agronomy and Plant Breeding, College of Agricultural and Natural Resources, University of Tehran, Karaj, Iran; Department of Agrobiotechnology, Agrarian Technological Institute, RUDN University, Moscow, Russia; Corresponding author at: Division of Plant Biotechnology, Department of Agronomy and Plant Breeding, College of Agricultural and Natural Resources, University of Tehran, Karaj, Iran.Department of Agrobiotechnology, Agrarian Technological Institute, RUDN University, Moscow, RussiaFerula sp. has achieved widespread fame as a producer of specialized terpenoids used as raw materials in fragrances, food, cosmetics, and pharmaceutical industries. Since ancient times, Ferula species has been utilized to treat various health issues, such as asthma, toothache, inflammation, cancer, and digestive disorders. Besides, a growing body of research proves the healing efficacy of Ferula plants in treating modern diseases, such as multiple sclerosis (MS), HIV, and COVID19. The major challenge surrounding the commercialization of Ferula-derived terpenoids is their low quantity in Ferula plants. This necessitates the exploitation of approaches to circumvent this barrier and to enhance their production level to meet the continuous demands of industries for Ferula terpenoids. Recently, via functional genomics, omics technologies, and high-throughput analytical techniques, our understanding about terpenoid biosynthesis and regulation has been deepened, paving the way for the overproduction of target terpenoids. This review examines the potential of hairy root culture, CRISPR/Cas-mediated genome editing, and metabolic engineering, including gene overexpression and enzyme engineering, for enhancing Ferula capacity tailored to industrial and medicinal needs. The strategies present here, except hairy root culture, have never been proposed or applied in Ferula species. In its ultimate form, the proposed strategies are expected to reach large-scale terpenoid production.http://www.sciencedirect.com/science/article/pii/S2214662825000234CRISPR/CasFeedback inhibitionFerulaHairy root cultureMetabolic engineeringSynthetic metabolon |
| spellingShingle | Zahra Aghaali Jun-Li Yang Mohammad Reza Naghavi Meisam Zargar Removing limitations surrounding terpenoid biosynthesis by biotechnological techniques in Ferula sp.: A review Current Plant Biology CRISPR/Cas Feedback inhibition Ferula Hairy root culture Metabolic engineering Synthetic metabolon |
| title | Removing limitations surrounding terpenoid biosynthesis by biotechnological techniques in Ferula sp.: A review |
| title_full | Removing limitations surrounding terpenoid biosynthesis by biotechnological techniques in Ferula sp.: A review |
| title_fullStr | Removing limitations surrounding terpenoid biosynthesis by biotechnological techniques in Ferula sp.: A review |
| title_full_unstemmed | Removing limitations surrounding terpenoid biosynthesis by biotechnological techniques in Ferula sp.: A review |
| title_short | Removing limitations surrounding terpenoid biosynthesis by biotechnological techniques in Ferula sp.: A review |
| title_sort | removing limitations surrounding terpenoid biosynthesis by biotechnological techniques in ferula sp a review |
| topic | CRISPR/Cas Feedback inhibition Ferula Hairy root culture Metabolic engineering Synthetic metabolon |
| url | http://www.sciencedirect.com/science/article/pii/S2214662825000234 |
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