Isolation and Identification of Chemical Constituents from Zhideke Granules by Ultra-Performance Liquid Chromatography Coupled with Mass Spectrometry
Chemical constituents from Zhideke granules were rapidly isolated and identified by ultra-performance liquid chromatography (UPLC) coupled with hybrid quadrupole-orbitrap mass spectrometry (MS) in positive and negative ion modes using both full scan and two-stage threshold-triggered mass modes. The...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2020/8889607 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832567868886089728 |
|---|---|
| author | Guangqiang Huang Jie Liang Xiaosi Chen Jing Lin Jinyu Wei Dongfang Huang Yushan Zhou Zhengyi Sun Lichun Zhao |
| author_facet | Guangqiang Huang Jie Liang Xiaosi Chen Jing Lin Jinyu Wei Dongfang Huang Yushan Zhou Zhengyi Sun Lichun Zhao |
| author_sort | Guangqiang Huang |
| collection | DOAJ |
| description | Chemical constituents from Zhideke granules were rapidly isolated and identified by ultra-performance liquid chromatography (UPLC) coupled with hybrid quadrupole-orbitrap mass spectrometry (MS) in positive and negative ion modes using both full scan and two-stage threshold-triggered mass modes. The secondary fragment ion information of the target compound was selected and compared with the compound reported in databases and related literatures to further confirm the possible compounds. A total of 47 chemical constituents were identified from the ethyl acetate extract of Zhideke granules, including 21 flavonoids and glycosides, 9 organic acids, 4 volatile components, 3 nitrogen-containing compounds, and 10 other compounds according to the fragmentation patterns, relevant literature, and MS data. The result provides a new method for the analysis of chemical constituents of Zhideke granules which laid the foundation for quality control and the study of pharmacodynamic materials of Zhideke granules. |
| format | Article |
| id | doaj-art-6601d25116494e3badb5943ebf577936 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-6601d25116494e3badb5943ebf5779362025-02-03T01:00:18ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732020-01-01202010.1155/2020/88896078889607Isolation and Identification of Chemical Constituents from Zhideke Granules by Ultra-Performance Liquid Chromatography Coupled with Mass SpectrometryGuangqiang Huang0Jie Liang1Xiaosi Chen2Jing Lin3Jinyu Wei4Dongfang Huang5Yushan Zhou6Zhengyi Sun7Lichun Zhao8College of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, ChinaRuikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530011, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, ChinaChemical constituents from Zhideke granules were rapidly isolated and identified by ultra-performance liquid chromatography (UPLC) coupled with hybrid quadrupole-orbitrap mass spectrometry (MS) in positive and negative ion modes using both full scan and two-stage threshold-triggered mass modes. The secondary fragment ion information of the target compound was selected and compared with the compound reported in databases and related literatures to further confirm the possible compounds. A total of 47 chemical constituents were identified from the ethyl acetate extract of Zhideke granules, including 21 flavonoids and glycosides, 9 organic acids, 4 volatile components, 3 nitrogen-containing compounds, and 10 other compounds according to the fragmentation patterns, relevant literature, and MS data. The result provides a new method for the analysis of chemical constituents of Zhideke granules which laid the foundation for quality control and the study of pharmacodynamic materials of Zhideke granules.http://dx.doi.org/10.1155/2020/8889607 |
| spellingShingle | Guangqiang Huang Jie Liang Xiaosi Chen Jing Lin Jinyu Wei Dongfang Huang Yushan Zhou Zhengyi Sun Lichun Zhao Isolation and Identification of Chemical Constituents from Zhideke Granules by Ultra-Performance Liquid Chromatography Coupled with Mass Spectrometry Journal of Analytical Methods in Chemistry |
| title | Isolation and Identification of Chemical Constituents from Zhideke Granules by Ultra-Performance Liquid Chromatography Coupled with Mass Spectrometry |
| title_full | Isolation and Identification of Chemical Constituents from Zhideke Granules by Ultra-Performance Liquid Chromatography Coupled with Mass Spectrometry |
| title_fullStr | Isolation and Identification of Chemical Constituents from Zhideke Granules by Ultra-Performance Liquid Chromatography Coupled with Mass Spectrometry |
| title_full_unstemmed | Isolation and Identification of Chemical Constituents from Zhideke Granules by Ultra-Performance Liquid Chromatography Coupled with Mass Spectrometry |
| title_short | Isolation and Identification of Chemical Constituents from Zhideke Granules by Ultra-Performance Liquid Chromatography Coupled with Mass Spectrometry |
| title_sort | isolation and identification of chemical constituents from zhideke granules by ultra performance liquid chromatography coupled with mass spectrometry |
| url | http://dx.doi.org/10.1155/2020/8889607 |
| work_keys_str_mv | AT guangqianghuang isolationandidentificationofchemicalconstituentsfromzhidekegranulesbyultraperformanceliquidchromatographycoupledwithmassspectrometry AT jieliang isolationandidentificationofchemicalconstituentsfromzhidekegranulesbyultraperformanceliquidchromatographycoupledwithmassspectrometry AT xiaosichen isolationandidentificationofchemicalconstituentsfromzhidekegranulesbyultraperformanceliquidchromatographycoupledwithmassspectrometry AT jinglin isolationandidentificationofchemicalconstituentsfromzhidekegranulesbyultraperformanceliquidchromatographycoupledwithmassspectrometry AT jinyuwei isolationandidentificationofchemicalconstituentsfromzhidekegranulesbyultraperformanceliquidchromatographycoupledwithmassspectrometry AT dongfanghuang isolationandidentificationofchemicalconstituentsfromzhidekegranulesbyultraperformanceliquidchromatographycoupledwithmassspectrometry AT yushanzhou isolationandidentificationofchemicalconstituentsfromzhidekegranulesbyultraperformanceliquidchromatographycoupledwithmassspectrometry AT zhengyisun isolationandidentificationofchemicalconstituentsfromzhidekegranulesbyultraperformanceliquidchromatographycoupledwithmassspectrometry AT lichunzhao isolationandidentificationofchemicalconstituentsfromzhidekegranulesbyultraperformanceliquidchromatographycoupledwithmassspectrometry |