Study on Carbohydrate Metabolism in Adult Zebrafish (Danio rerio)
Excessive carbohydrate intake leads to metabolic disorders in fish. However, few literatures have reported the appropriate carbohydrate level for zebrafish, and the metabolic response to dietary carbohydrate remains largely unknown in zebrafish. This study assessed the responses of zebrafish and zeb...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-01-01
|
| Series: | Aquaculture Nutrition |
| Online Access: | http://dx.doi.org/10.1155/2023/1397508 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850158768630792192 |
|---|---|
| author | Longwei Xi Qisheng Lu Yulong Liu Yulong Gong Haokun Liu Junyan Jin Zhimin Zhang Yunxia Yang Xiaoming Zhu Dong Han Shouqi Xie |
| author_facet | Longwei Xi Qisheng Lu Yulong Liu Yulong Gong Haokun Liu Junyan Jin Zhimin Zhang Yunxia Yang Xiaoming Zhu Dong Han Shouqi Xie |
| author_sort | Longwei Xi |
| collection | DOAJ |
| description | Excessive carbohydrate intake leads to metabolic disorders in fish. However, few literatures have reported the appropriate carbohydrate level for zebrafish, and the metabolic response to dietary carbohydrate remains largely unknown in zebrafish. This study assessed the responses of zebrafish and zebrafish liver cell line (ZFL) to different carbohydrate levels. In vivo results showed that ≥30% dietary dextrin levels significantly increased the plasma glucose content, activated the expression of hepatic glycolysis-related genes, and inhibited the expression of hepatic gluconeogenesis-related genes in zebrafish. Oil red O staining, triglyceride content, and Hematoxylin-Eosin staining results showed that dietary dextrin levels of ≥30% significantly increased lipid accumulation and liver damage, as well as processes related to glycolipid metabolism and inflammation in zebrafish. In ZFL, the transcription factor sterol regulatory element binding protein-1c signal intensity, 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY 493/503) signal intensity, and triglyceride content were also significantly increased when incubated in high glucose, along with abnormal glycolipid metabolism and increased inflammation-related genes. In conclusion, we demonstrated that the maximum dietary carbohydrate level in adult zebrafish should be less than 30%. Excess dietary carbohydrates (30%–50%) caused hepatic steatosis and damage to zebrafish, similar to that seen in aquaculture species. Thus, this study assessed responses to different carbohydrate levels in zebrafish and illustrated that zebrafish is an optimal model for investigating glucose metabolism in some aquatic animals. |
| format | Article |
| id | doaj-art-65feafa1d5d947f89ce78b9f8c0eb635 |
| institution | OA Journals |
| issn | 1365-2095 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Aquaculture Nutrition |
| spelling | doaj-art-65feafa1d5d947f89ce78b9f8c0eb6352025-08-20T02:23:46ZengWileyAquaculture Nutrition1365-20952023-01-01202310.1155/2023/1397508Study on Carbohydrate Metabolism in Adult Zebrafish (Danio rerio)Longwei Xi0Qisheng Lu1Yulong Liu2Yulong Gong3Haokun Liu4Junyan Jin5Zhimin Zhang6Yunxia Yang7Xiaoming Zhu8Dong Han9Shouqi Xie10State Key Laboratory of Freshwater Ecology and BiotechnologyState Key Laboratory of Freshwater Ecology and BiotechnologyState Key Laboratory of Freshwater Ecology and BiotechnologyState Key Laboratory of Freshwater Ecology and BiotechnologyState Key Laboratory of Freshwater Ecology and BiotechnologyState Key Laboratory of Freshwater Ecology and BiotechnologyState Key Laboratory of Freshwater Ecology and BiotechnologyState Key Laboratory of Freshwater Ecology and BiotechnologyState Key Laboratory of Freshwater Ecology and BiotechnologyState Key Laboratory of Freshwater Ecology and BiotechnologyState Key Laboratory of Freshwater Ecology and BiotechnologyExcessive carbohydrate intake leads to metabolic disorders in fish. However, few literatures have reported the appropriate carbohydrate level for zebrafish, and the metabolic response to dietary carbohydrate remains largely unknown in zebrafish. This study assessed the responses of zebrafish and zebrafish liver cell line (ZFL) to different carbohydrate levels. In vivo results showed that ≥30% dietary dextrin levels significantly increased the plasma glucose content, activated the expression of hepatic glycolysis-related genes, and inhibited the expression of hepatic gluconeogenesis-related genes in zebrafish. Oil red O staining, triglyceride content, and Hematoxylin-Eosin staining results showed that dietary dextrin levels of ≥30% significantly increased lipid accumulation and liver damage, as well as processes related to glycolipid metabolism and inflammation in zebrafish. In ZFL, the transcription factor sterol regulatory element binding protein-1c signal intensity, 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY 493/503) signal intensity, and triglyceride content were also significantly increased when incubated in high glucose, along with abnormal glycolipid metabolism and increased inflammation-related genes. In conclusion, we demonstrated that the maximum dietary carbohydrate level in adult zebrafish should be less than 30%. Excess dietary carbohydrates (30%–50%) caused hepatic steatosis and damage to zebrafish, similar to that seen in aquaculture species. Thus, this study assessed responses to different carbohydrate levels in zebrafish and illustrated that zebrafish is an optimal model for investigating glucose metabolism in some aquatic animals.http://dx.doi.org/10.1155/2023/1397508 |
| spellingShingle | Longwei Xi Qisheng Lu Yulong Liu Yulong Gong Haokun Liu Junyan Jin Zhimin Zhang Yunxia Yang Xiaoming Zhu Dong Han Shouqi Xie Study on Carbohydrate Metabolism in Adult Zebrafish (Danio rerio) Aquaculture Nutrition |
| title | Study on Carbohydrate Metabolism in Adult Zebrafish (Danio rerio) |
| title_full | Study on Carbohydrate Metabolism in Adult Zebrafish (Danio rerio) |
| title_fullStr | Study on Carbohydrate Metabolism in Adult Zebrafish (Danio rerio) |
| title_full_unstemmed | Study on Carbohydrate Metabolism in Adult Zebrafish (Danio rerio) |
| title_short | Study on Carbohydrate Metabolism in Adult Zebrafish (Danio rerio) |
| title_sort | study on carbohydrate metabolism in adult zebrafish danio rerio |
| url | http://dx.doi.org/10.1155/2023/1397508 |
| work_keys_str_mv | AT longweixi studyoncarbohydratemetabolisminadultzebrafishdaniorerio AT qishenglu studyoncarbohydratemetabolisminadultzebrafishdaniorerio AT yulongliu studyoncarbohydratemetabolisminadultzebrafishdaniorerio AT yulonggong studyoncarbohydratemetabolisminadultzebrafishdaniorerio AT haokunliu studyoncarbohydratemetabolisminadultzebrafishdaniorerio AT junyanjin studyoncarbohydratemetabolisminadultzebrafishdaniorerio AT zhiminzhang studyoncarbohydratemetabolisminadultzebrafishdaniorerio AT yunxiayang studyoncarbohydratemetabolisminadultzebrafishdaniorerio AT xiaomingzhu studyoncarbohydratemetabolisminadultzebrafishdaniorerio AT donghan studyoncarbohydratemetabolisminadultzebrafishdaniorerio AT shouqixie studyoncarbohydratemetabolisminadultzebrafishdaniorerio |