Functional analysis of Parabacteroides distasonis F4: a novel probiotic strain linked to calf growth and rumen fermentation
Abstract Background Rumen microorganisms are key regulators of ruminant growth and production performance. Identifying probiotic candidates through microbial culturomics presents a promising strategy for improving ruminant production performance. Our previous study identified significant differences...
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BMC
2025-04-01
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| Series: | Journal of Animal Science and Biotechnology |
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| Online Access: | https://doi.org/10.1186/s40104-025-01182-0 |
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| author | Xiaoran Feng Yunlong Liu Shengyang Xu Junnan Ma Hao Yuan Haixin Wang Jiachen Hu Sijie Jin Shanji Liu Jin Zhong Tao Ma Yan Tu |
| author_facet | Xiaoran Feng Yunlong Liu Shengyang Xu Junnan Ma Hao Yuan Haixin Wang Jiachen Hu Sijie Jin Shanji Liu Jin Zhong Tao Ma Yan Tu |
| author_sort | Xiaoran Feng |
| collection | DOAJ |
| description | Abstract Background Rumen microorganisms are key regulators of ruminant growth and production performance. Identifying probiotic candidates through microbial culturomics presents a promising strategy for improving ruminant production performance. Our previous study identified significant differences in rumen microbial communities of Holstein calves with varying average daily gain (ADG). This study aims to identify a target strain based on the findings from multi-omics analysis and literature review, isolating and evaluating the target microbial strains from both the rumen and hindgut contents for their probiotic potential. Results Parabacteroides distasonis, a strain closely associated with ADG, was successfully isolated from calf rumen content cultured with Fastidious Anaerobe Agar (FAA) medium and named Parabacteroides distasonis F4. Whole-genome sequencing and pan-genome analysis showed that P. distasonis F4 possesses a core functional potential for carbohydrate and amino acid metabolism, with the ability to produce propionate, acetate, and lactate. The results of targeted and untargeted metabolomics further validated the organic acid production and metabolic pathways of P. distasonis F4. An in vitro simulated rumen fermentation test showed that supplementation with P. distasonis F4 significantly altered rumen microbial community structure and increased the molar proportions of propionate and butyrate in the rumen. Furthermore, an in vivo study demonstrated that dietary supplementation with P. distasonis F4 significantly increased the ADG of pre-weaning calves. Conclusions This study represents the first isolation of P. distasonis F4 from rumen, highlighting its potential as a probiotic strain for improving rumen development and growth performance in ruminants. |
| format | Article |
| id | doaj-art-65f8b957f1bf4e6a87327222c7003a3e |
| institution | DOAJ |
| issn | 2049-1891 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | BMC |
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| series | Journal of Animal Science and Biotechnology |
| spelling | doaj-art-65f8b957f1bf4e6a87327222c7003a3e2025-08-20T03:07:44ZengBMCJournal of Animal Science and Biotechnology2049-18912025-04-0116111710.1186/s40104-025-01182-0Functional analysis of Parabacteroides distasonis F4: a novel probiotic strain linked to calf growth and rumen fermentationXiaoran Feng0Yunlong Liu1Shengyang Xu2Junnan Ma3Hao Yuan4Haixin Wang5Jiachen Hu6Sijie Jin7Shanji Liu8Jin Zhong9Tao Ma10Yan Tu11Beijing Key Laboratory for Dairy Cow Nutrition, Institute of Feed Research, Chinese Academy of Agricultural SciencesBeijing Key Laboratory for Dairy Cow Nutrition, Institute of Feed Research, Chinese Academy of Agricultural SciencesBeijing Key Laboratory for Dairy Cow Nutrition, Institute of Feed Research, Chinese Academy of Agricultural SciencesBeijing Key Laboratory for Dairy Cow Nutrition, Institute of Feed Research, Chinese Academy of Agricultural SciencesBeijing Key Laboratory for Dairy Cow Nutrition, Institute of Feed Research, Chinese Academy of Agricultural SciencesBeijing Key Laboratory for Dairy Cow Nutrition, Institute of Feed Research, Chinese Academy of Agricultural SciencesState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of SciencesBeijing Key Laboratory for Dairy Cow Nutrition, Institute of Feed Research, Chinese Academy of Agricultural SciencesBeijing Key Laboratory for Dairy Cow Nutrition, Institute of Feed Research, Chinese Academy of Agricultural SciencesAbstract Background Rumen microorganisms are key regulators of ruminant growth and production performance. Identifying probiotic candidates through microbial culturomics presents a promising strategy for improving ruminant production performance. Our previous study identified significant differences in rumen microbial communities of Holstein calves with varying average daily gain (ADG). This study aims to identify a target strain based on the findings from multi-omics analysis and literature review, isolating and evaluating the target microbial strains from both the rumen and hindgut contents for their probiotic potential. Results Parabacteroides distasonis, a strain closely associated with ADG, was successfully isolated from calf rumen content cultured with Fastidious Anaerobe Agar (FAA) medium and named Parabacteroides distasonis F4. Whole-genome sequencing and pan-genome analysis showed that P. distasonis F4 possesses a core functional potential for carbohydrate and amino acid metabolism, with the ability to produce propionate, acetate, and lactate. The results of targeted and untargeted metabolomics further validated the organic acid production and metabolic pathways of P. distasonis F4. An in vitro simulated rumen fermentation test showed that supplementation with P. distasonis F4 significantly altered rumen microbial community structure and increased the molar proportions of propionate and butyrate in the rumen. Furthermore, an in vivo study demonstrated that dietary supplementation with P. distasonis F4 significantly increased the ADG of pre-weaning calves. Conclusions This study represents the first isolation of P. distasonis F4 from rumen, highlighting its potential as a probiotic strain for improving rumen development and growth performance in ruminants.https://doi.org/10.1186/s40104-025-01182-0Pan-genomeParabacteroides distasonisProbioticRumen fermentation |
| spellingShingle | Xiaoran Feng Yunlong Liu Shengyang Xu Junnan Ma Hao Yuan Haixin Wang Jiachen Hu Sijie Jin Shanji Liu Jin Zhong Tao Ma Yan Tu Functional analysis of Parabacteroides distasonis F4: a novel probiotic strain linked to calf growth and rumen fermentation Journal of Animal Science and Biotechnology Pan-genome Parabacteroides distasonis Probiotic Rumen fermentation |
| title | Functional analysis of Parabacteroides distasonis F4: a novel probiotic strain linked to calf growth and rumen fermentation |
| title_full | Functional analysis of Parabacteroides distasonis F4: a novel probiotic strain linked to calf growth and rumen fermentation |
| title_fullStr | Functional analysis of Parabacteroides distasonis F4: a novel probiotic strain linked to calf growth and rumen fermentation |
| title_full_unstemmed | Functional analysis of Parabacteroides distasonis F4: a novel probiotic strain linked to calf growth and rumen fermentation |
| title_short | Functional analysis of Parabacteroides distasonis F4: a novel probiotic strain linked to calf growth and rumen fermentation |
| title_sort | functional analysis of parabacteroides distasonis f4 a novel probiotic strain linked to calf growth and rumen fermentation |
| topic | Pan-genome Parabacteroides distasonis Probiotic Rumen fermentation |
| url | https://doi.org/10.1186/s40104-025-01182-0 |
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