Novel biocompatible magnetron-sputtered silver coating for enhanced antibacterial properties and osteogenesis in vitro
Abstract Peri-implant infection is a serious complication in orthopedic surgery. This study aimed to reduce the incidence of peri-implant infection by developing a durable and safe antibacterial silver coating. We compared the antibacterial properties and process controllability of various coating t...
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Nature Portfolio
2024-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-77270-4 |
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| author | Talante Juma Hongbin Wang Xiangyu Cao Qiwei Wang Hao Wang Bingxiao Yu Xiaotong Bao Wenjuan Rong Hua Tian Yongping Cao |
| author_facet | Talante Juma Hongbin Wang Xiangyu Cao Qiwei Wang Hao Wang Bingxiao Yu Xiaotong Bao Wenjuan Rong Hua Tian Yongping Cao |
| author_sort | Talante Juma |
| collection | DOAJ |
| description | Abstract Peri-implant infection is a serious complication in orthopedic surgery. This study aimed to reduce the incidence of peri-implant infection by developing a durable and safe antibacterial silver coating. We compared the antibacterial properties and process controllability of various coating techniques to identify the most effective method for silver coating. We refined substrate treatment techniques and coating thicknesses through antibacterial and scratch tests. Scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) were used to analyze the coating’s morphology and composition. Micron-sized magnetron sputtering silver coating samples underwent in vitro antibacterial testing, cytotoxicity testing, silver ion release testing, and osteogenic testing using membrane contact culture, CCK-8 assay, inductively coupled plasma (ICP) detection, and alkaline phosphatase (ALP) activity/osteogenic gene PCR. Magnetron sputtering demonstrated superior antibacterial properties, uniformity, and process controllability compared to alternative techniques. The optimal adhesion strength was achieved with a 0.5 μm coating thickness and a 400 mesh sandpaper pretreatment process, without compromising antibacterial efficacy. The coating showed near-perfect antiseptic results in antibacterial and anti-biofilm tests. Fibroblasts cultured in silver ion precipitation medium exhibited growth rates of 89% on day 30 and 88% on day 90, compared to 95% in the control group. The osteogenic test indicated that the magnetron sputtering silver coating promotes osteogenesis effectively. Our study demonstrated that micron-sized magnetron sputtering silver coating has potential for clinical use to prevent peri-implant infections in the future. |
| format | Article |
| id | doaj-art-65f8284ada2a45caa7eb7ec885ed97ec |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-11-01 |
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| spelling | doaj-art-65f8284ada2a45caa7eb7ec885ed97ec2025-08-20T02:22:26ZengNature PortfolioScientific Reports2045-23222024-11-0114111410.1038/s41598-024-77270-4Novel biocompatible magnetron-sputtered silver coating for enhanced antibacterial properties and osteogenesis in vitroTalante Juma0Hongbin Wang1Xiangyu Cao2Qiwei Wang3Hao Wang4Bingxiao Yu5Xiaotong Bao6Wenjuan Rong7Hua Tian8Yongping Cao9Orthopedic Department, Peking University First HospitalOrthopedic Department, Peking University First HospitalOrthopedic Department, Peking University Third HospitalOrthopedic Department, Peking University First HospitalOrthopedic Department, Peking University First HospitalOrthopedic Department, Peking University Shougang HospitalCapital Aerospace Machinery Corporation LimitedBeijing National Innovation Institute of Lightweight LTDOrthopedic Department, Peking University Third HospitalOrthopedic Department, Peking University First HospitalAbstract Peri-implant infection is a serious complication in orthopedic surgery. This study aimed to reduce the incidence of peri-implant infection by developing a durable and safe antibacterial silver coating. We compared the antibacterial properties and process controllability of various coating techniques to identify the most effective method for silver coating. We refined substrate treatment techniques and coating thicknesses through antibacterial and scratch tests. Scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) were used to analyze the coating’s morphology and composition. Micron-sized magnetron sputtering silver coating samples underwent in vitro antibacterial testing, cytotoxicity testing, silver ion release testing, and osteogenic testing using membrane contact culture, CCK-8 assay, inductively coupled plasma (ICP) detection, and alkaline phosphatase (ALP) activity/osteogenic gene PCR. Magnetron sputtering demonstrated superior antibacterial properties, uniformity, and process controllability compared to alternative techniques. The optimal adhesion strength was achieved with a 0.5 μm coating thickness and a 400 mesh sandpaper pretreatment process, without compromising antibacterial efficacy. The coating showed near-perfect antiseptic results in antibacterial and anti-biofilm tests. Fibroblasts cultured in silver ion precipitation medium exhibited growth rates of 89% on day 30 and 88% on day 90, compared to 95% in the control group. The osteogenic test indicated that the magnetron sputtering silver coating promotes osteogenesis effectively. Our study demonstrated that micron-sized magnetron sputtering silver coating has potential for clinical use to prevent peri-implant infections in the future.https://doi.org/10.1038/s41598-024-77270-4 |
| spellingShingle | Talante Juma Hongbin Wang Xiangyu Cao Qiwei Wang Hao Wang Bingxiao Yu Xiaotong Bao Wenjuan Rong Hua Tian Yongping Cao Novel biocompatible magnetron-sputtered silver coating for enhanced antibacterial properties and osteogenesis in vitro Scientific Reports |
| title | Novel biocompatible magnetron-sputtered silver coating for enhanced antibacterial properties and osteogenesis in vitro |
| title_full | Novel biocompatible magnetron-sputtered silver coating for enhanced antibacterial properties and osteogenesis in vitro |
| title_fullStr | Novel biocompatible magnetron-sputtered silver coating for enhanced antibacterial properties and osteogenesis in vitro |
| title_full_unstemmed | Novel biocompatible magnetron-sputtered silver coating for enhanced antibacterial properties and osteogenesis in vitro |
| title_short | Novel biocompatible magnetron-sputtered silver coating for enhanced antibacterial properties and osteogenesis in vitro |
| title_sort | novel biocompatible magnetron sputtered silver coating for enhanced antibacterial properties and osteogenesis in vitro |
| url | https://doi.org/10.1038/s41598-024-77270-4 |
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