Gene Expression Profiling of the Paracrine Effects of Uterine Natural Killer Cells on Human Endometrial Epithelial Cells
The endometrium contains a population of immune cells that undergo changes during implantation and pregnancy. The majority of these cells are uterine natural killer (uNK) cells; however, it is unclear how these cells interact with endometrial epithelial cells. Therefore, we investigated the paracrin...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2014/393707 |
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| author | Xin Gong Zhenzhen Chen Yanxia Liu Qiudan Lu Zhe Jin |
| author_facet | Xin Gong Zhenzhen Chen Yanxia Liu Qiudan Lu Zhe Jin |
| author_sort | Xin Gong |
| collection | DOAJ |
| description | The endometrium contains a population of immune cells that undergo changes during implantation and pregnancy. The majority of these cells are uterine natural killer (uNK) cells; however, it is unclear how these cells interact with endometrial epithelial cells. Therefore, we investigated the paracrine effects of the uNK cell-secretion medium on the gene expression profile of endometrial epithelial cells in vitro through microarray analysis. Our results, which were verified by qRT-PCR and western blot, revealed that soluble factors from uNK cells alter the gene expression profiles of epithelial cells. The upregulated genes included interleukin-15 (IL-15) and interleukin-15 receptor alpha (IL-15RA), which result in a loop that stimulates uNK cell proliferation. In addition, vascular endothelial growth factor C (VEGF-C) and chemokine (C-X-C motif) ligand 10 (CXCL-10) were also determined to be upregulated in epithelial cells, which suggests that uNK cells work synergistically with epithelial cells to support implantation and pregnancy. In addition, oriental herbal medicines have been used to treat infertility since ancient times; however, we failed to find that Zi Dan Yin can regulate these endometrial paracrine effects. |
| format | Article |
| id | doaj-art-65f2b8dc7e7d4d7c8bf6a068b4813e0c |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-65f2b8dc7e7d4d7c8bf6a068b4813e0c2025-08-20T03:34:17ZengWileyInternational Journal of Endocrinology1687-83371687-83452014-01-01201410.1155/2014/393707393707Gene Expression Profiling of the Paracrine Effects of Uterine Natural Killer Cells on Human Endometrial Epithelial CellsXin Gong0Zhenzhen Chen1Yanxia Liu2Qiudan Lu3Zhe Jin4Reproductive Endocrinology Centre, Dongfang Hospital of Beijing University of Chinese Medicine, No. 6 Fangxingyuan 1 Qu, Fengtai District, Beijing 100078, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 6 Zhonghuan South Road, Wangjing, Chaoyang District, Beijing 100102, ChinaReproductive Endocrinology Centre, Dongfang Hospital of Beijing University of Chinese Medicine, No. 6 Fangxingyuan 1 Qu, Fengtai District, Beijing 100078, ChinaReproductive Endocrinology Centre, Dongfang Hospital of Beijing University of Chinese Medicine, No. 6 Fangxingyuan 1 Qu, Fengtai District, Beijing 100078, ChinaReproductive Endocrinology Centre, Dongfang Hospital of Beijing University of Chinese Medicine, No. 6 Fangxingyuan 1 Qu, Fengtai District, Beijing 100078, ChinaThe endometrium contains a population of immune cells that undergo changes during implantation and pregnancy. The majority of these cells are uterine natural killer (uNK) cells; however, it is unclear how these cells interact with endometrial epithelial cells. Therefore, we investigated the paracrine effects of the uNK cell-secretion medium on the gene expression profile of endometrial epithelial cells in vitro through microarray analysis. Our results, which were verified by qRT-PCR and western blot, revealed that soluble factors from uNK cells alter the gene expression profiles of epithelial cells. The upregulated genes included interleukin-15 (IL-15) and interleukin-15 receptor alpha (IL-15RA), which result in a loop that stimulates uNK cell proliferation. In addition, vascular endothelial growth factor C (VEGF-C) and chemokine (C-X-C motif) ligand 10 (CXCL-10) were also determined to be upregulated in epithelial cells, which suggests that uNK cells work synergistically with epithelial cells to support implantation and pregnancy. In addition, oriental herbal medicines have been used to treat infertility since ancient times; however, we failed to find that Zi Dan Yin can regulate these endometrial paracrine effects.http://dx.doi.org/10.1155/2014/393707 |
| spellingShingle | Xin Gong Zhenzhen Chen Yanxia Liu Qiudan Lu Zhe Jin Gene Expression Profiling of the Paracrine Effects of Uterine Natural Killer Cells on Human Endometrial Epithelial Cells International Journal of Endocrinology |
| title | Gene Expression Profiling of the Paracrine Effects of Uterine Natural Killer Cells on Human Endometrial Epithelial Cells |
| title_full | Gene Expression Profiling of the Paracrine Effects of Uterine Natural Killer Cells on Human Endometrial Epithelial Cells |
| title_fullStr | Gene Expression Profiling of the Paracrine Effects of Uterine Natural Killer Cells on Human Endometrial Epithelial Cells |
| title_full_unstemmed | Gene Expression Profiling of the Paracrine Effects of Uterine Natural Killer Cells on Human Endometrial Epithelial Cells |
| title_short | Gene Expression Profiling of the Paracrine Effects of Uterine Natural Killer Cells on Human Endometrial Epithelial Cells |
| title_sort | gene expression profiling of the paracrine effects of uterine natural killer cells on human endometrial epithelial cells |
| url | http://dx.doi.org/10.1155/2014/393707 |
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