SP140L, an Evolutionarily Recent Member of the SP100 Family, Is an Autoantigen in Primary Biliary Cirrhosis
The SP100 family members comprise a set of closely related genes on chromosome 2q37.1. The widely expressed SP100 and the leukocyte-specific proteins SP110 and SP140 have been associated with transcriptional regulation and various human diseases. Here, we have characterized the SP100 family member S...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/526518 |
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| author | Mario Saare Uku Hämarik Rainis Venta Marina Panarina Chiara Zucchelli Maire Pihlap Anu Remm Kai Kisand Urve Toots Kaidi Möll Riina Salupere Giovanna Musco Raivo Uibo Pärt Peterson |
| author_facet | Mario Saare Uku Hämarik Rainis Venta Marina Panarina Chiara Zucchelli Maire Pihlap Anu Remm Kai Kisand Urve Toots Kaidi Möll Riina Salupere Giovanna Musco Raivo Uibo Pärt Peterson |
| author_sort | Mario Saare |
| collection | DOAJ |
| description | The SP100 family members comprise a set of closely related genes on chromosome 2q37.1. The widely expressed SP100 and the leukocyte-specific proteins SP110 and SP140 have been associated with transcriptional regulation and various human diseases. Here, we have characterized the SP100 family member SP140L. The genome sequence analysis showed the formation of SP140L gene through rearrangements of the two neighboring genes, SP100 and SP140, during the evolution of higher primates. The SP140L expression is interferon-inducible with high transcript levels in B cells and other peripheral blood mononuclear cells. Subcellularly, SP140L colocalizes with SP100 and SP140 in nuclear structures that are devoid of SP110, PML, or p300 proteins. Similarly to SP100 and SP140 protein, we detected serum autoantibodies to SP140L in patients with primary biliary cirrhosis using luciferase immunoprecipitation system and immunoblotting assays. In conclusion, our results show that SP140L is phylogenetically recent member of SP100 proteins and acts as an autoantigen in primary biliary cirrhosis patients. |
| format | Article |
| id | doaj-art-65edfa692adb4cb58162b83a2777a992 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-65edfa692adb4cb58162b83a2777a9922025-02-03T05:57:53ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/526518526518SP140L, an Evolutionarily Recent Member of the SP100 Family, Is an Autoantigen in Primary Biliary CirrhosisMario Saare0Uku Hämarik1Rainis Venta2Marina Panarina3Chiara Zucchelli4Maire Pihlap5Anu Remm6Kai Kisand7Urve Toots8Kaidi Möll9Riina Salupere10Giovanna Musco11Raivo Uibo12Pärt Peterson13Molecular Pathology Group, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411 Tartu, EstoniaMolecular Pathology Group, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411 Tartu, EstoniaInstitute of Technology, University of Tartu, 50411 Tartu, EstoniaDepartment of Immunology, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411 Tartu, EstoniaBiomolecular NMR Laboratory, Dulbecco Telethon Institute c/o S. Raffaele Scientific Institute, 20132 Milan, ItalyMolecular Pathology Group, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411 Tartu, EstoniaMolecular Pathology Group, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411 Tartu, EstoniaMolecular Pathology Group, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411 Tartu, EstoniaIcosagen AS, 61713 Tartumaa, EstoniaIcosagen AS, 61713 Tartumaa, EstoniaDepartment of Internal Medicine, Tartu University Hospital, 51014 Tartu, EstoniaBiomolecular NMR Laboratory, Dulbecco Telethon Institute c/o S. Raffaele Scientific Institute, 20132 Milan, ItalyDepartment of Immunology, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411 Tartu, EstoniaMolecular Pathology Group, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411 Tartu, EstoniaThe SP100 family members comprise a set of closely related genes on chromosome 2q37.1. The widely expressed SP100 and the leukocyte-specific proteins SP110 and SP140 have been associated with transcriptional regulation and various human diseases. Here, we have characterized the SP100 family member SP140L. The genome sequence analysis showed the formation of SP140L gene through rearrangements of the two neighboring genes, SP100 and SP140, during the evolution of higher primates. The SP140L expression is interferon-inducible with high transcript levels in B cells and other peripheral blood mononuclear cells. Subcellularly, SP140L colocalizes with SP100 and SP140 in nuclear structures that are devoid of SP110, PML, or p300 proteins. Similarly to SP100 and SP140 protein, we detected serum autoantibodies to SP140L in patients with primary biliary cirrhosis using luciferase immunoprecipitation system and immunoblotting assays. In conclusion, our results show that SP140L is phylogenetically recent member of SP100 proteins and acts as an autoantigen in primary biliary cirrhosis patients.http://dx.doi.org/10.1155/2015/526518 |
| spellingShingle | Mario Saare Uku Hämarik Rainis Venta Marina Panarina Chiara Zucchelli Maire Pihlap Anu Remm Kai Kisand Urve Toots Kaidi Möll Riina Salupere Giovanna Musco Raivo Uibo Pärt Peterson SP140L, an Evolutionarily Recent Member of the SP100 Family, Is an Autoantigen in Primary Biliary Cirrhosis Journal of Immunology Research |
| title | SP140L, an Evolutionarily Recent Member of the SP100 Family, Is an Autoantigen in Primary Biliary Cirrhosis |
| title_full | SP140L, an Evolutionarily Recent Member of the SP100 Family, Is an Autoantigen in Primary Biliary Cirrhosis |
| title_fullStr | SP140L, an Evolutionarily Recent Member of the SP100 Family, Is an Autoantigen in Primary Biliary Cirrhosis |
| title_full_unstemmed | SP140L, an Evolutionarily Recent Member of the SP100 Family, Is an Autoantigen in Primary Biliary Cirrhosis |
| title_short | SP140L, an Evolutionarily Recent Member of the SP100 Family, Is an Autoantigen in Primary Biliary Cirrhosis |
| title_sort | sp140l an evolutionarily recent member of the sp100 family is an autoantigen in primary biliary cirrhosis |
| url | http://dx.doi.org/10.1155/2015/526518 |
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