The expression of immunohistochemical biomarkers PAX8 and CD117 in platinum resistant ovarian cancer at Saiful Anwar General Hospital, Malang, Indonesia
HIGHLIGHTS • Ovarian cancer is a gynecological disease commonly encountered by women, and resistance to platinum therapy is a significant challenge in its management • PAX8 and CD117 have shown potential as predictors of resistance in ovarian cancer. However, research on the expression of these b...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Airlangga
2025-04-01
|
| Series: | Majalah Obstetri dan Ginekologi |
| Subjects: | |
| Online Access: | https://e-journal.unair.ac.id/MOG/article/view/52881 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849733834075013120 |
|---|---|
| author | Robby Rinaldi Widodo Tatit Nurseta Edy Mustofa Onni Dwi Arianto R. A. Rose Khasana Dewi |
| author_facet | Robby Rinaldi Widodo Tatit Nurseta Edy Mustofa Onni Dwi Arianto R. A. Rose Khasana Dewi |
| author_sort | Robby Rinaldi Widodo |
| collection | DOAJ |
| description | HIGHLIGHTS
• Ovarian cancer is a gynecological disease commonly encountered by women, and resistance to platinum therapy is a significant challenge in its management
• PAX8 and CD117 have shown potential as predictors of resistance in ovarian cancer. However, research on the expression of these biomarkers concerning platinum resistance in ovarian cancer patients.
• The objective of this research is to assess the immunohistochemistry expression of CD117 and PAX8 in ovarian cancer and determine whether these expressions are linked to the development of platinum resistance.
ABSTRACT
Objective: Ovarian cancer is a gynecological disease commonly encountered by women, and resistance to platinum therapy is a significant challenge in its management. This study aims explore a monitoring method for post-operative ovarian carcinoma also evaluate the immunohistochemical expression of PAX8 and CD117 in ovarian cancer and identify their association with the occurrence of platinum resistance.
Materials and Methods: A cross-sectional approach to an observational analytical design, utilizing consecutive sampling which have met the inclusion and exclusion criteria. Tissue resulting from biopsy/surgical procedure which was made into a representative paraffin block was then subjected to immuno-histochemical examination in the Anatomical Pathology Department of Saiful Anwar Hospital Malang. PAX8 and CD117 expressions were analyzed using immunohistochemistry. The results of this study were assessed using ROC curves accompanied by Youden index calculation, determining sensitivity and specificity levels.
Results: In this research, the results were obtained that the area under the curve (AUC) for PAX8 and CD117 is 0.785 and 0.809, respectively. PAX8 expression demonstrates the positive predictive value, negative predictive value, sensitivity, specificity, and accuracy of 53.125%, 87.50%, 18.75%, 51.85%, and 60.0%, respectively. The expression of CD117 displays the following values: 71.4 percent, 72.73%, 70%, 72.73%, and 70% for the positive predictive value, negative predictive value, sensitivity, specificity, and accuracy, respectively.
Conclusion: According to this study, PAX8 and CD117 immunohistochemistry expression in ovarian cancer may act as prognostic biomarkers for platinum resistance. In this study there are several limitations that can be revised in future research. |
| format | Article |
| id | doaj-art-65e4e3953b3b4457b3e582bc8ebe54c3 |
| institution | DOAJ |
| issn | 0854-0381 2598-1013 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Majalah Obstetri dan Ginekologi |
| spelling | doaj-art-65e4e3953b3b4457b3e582bc8ebe54c32025-08-20T03:07:57ZengUniversitas AirlanggaMajalah Obstetri dan Ginekologi0854-03812598-10132025-04-0133111010.20473/mog.V33I12025.1-1051042The expression of immunohistochemical biomarkers PAX8 and CD117 in platinum resistant ovarian cancer at Saiful Anwar General Hospital, Malang, IndonesiaRobby Rinaldi Widodo0https://orcid.org/0009-0000-8664-5368Tatit Nurseta1https://orcid.org/0000-0001-7477-5181Edy Mustofa2https://orcid.org/0000-0002-4197-3250Onni Dwi Arianto3https://orcid.org/0009-0007-8694-246XR. A. Rose Khasana Dewi4https://orcid.org/0000-0001-6681-4216Department of Obstetrics and Gynecology, Faculty of Medicine, Brawijaya University, MalangDepartment of Obstetrics and Gynecology, Faculty of Medicine, Brawijaya University, MalangDepartment of Obstetrics and Gynecology, Faculty of Medicine, Brawijaya University, MalangDepartment of Obstetrics and Gynecology, Faculty of Medicine, Brawijaya University, MalangDepartment of Anatomic Pathology, Faculty of Medicine, Brawijaya University, MalangHIGHLIGHTS • Ovarian cancer is a gynecological disease commonly encountered by women, and resistance to platinum therapy is a significant challenge in its management • PAX8 and CD117 have shown potential as predictors of resistance in ovarian cancer. However, research on the expression of these biomarkers concerning platinum resistance in ovarian cancer patients. • The objective of this research is to assess the immunohistochemistry expression of CD117 and PAX8 in ovarian cancer and determine whether these expressions are linked to the development of platinum resistance. ABSTRACT Objective: Ovarian cancer is a gynecological disease commonly encountered by women, and resistance to platinum therapy is a significant challenge in its management. This study aims explore a monitoring method for post-operative ovarian carcinoma also evaluate the immunohistochemical expression of PAX8 and CD117 in ovarian cancer and identify their association with the occurrence of platinum resistance. Materials and Methods: A cross-sectional approach to an observational analytical design, utilizing consecutive sampling which have met the inclusion and exclusion criteria. Tissue resulting from biopsy/surgical procedure which was made into a representative paraffin block was then subjected to immuno-histochemical examination in the Anatomical Pathology Department of Saiful Anwar Hospital Malang. PAX8 and CD117 expressions were analyzed using immunohistochemistry. The results of this study were assessed using ROC curves accompanied by Youden index calculation, determining sensitivity and specificity levels. Results: In this research, the results were obtained that the area under the curve (AUC) for PAX8 and CD117 is 0.785 and 0.809, respectively. PAX8 expression demonstrates the positive predictive value, negative predictive value, sensitivity, specificity, and accuracy of 53.125%, 87.50%, 18.75%, 51.85%, and 60.0%, respectively. The expression of CD117 displays the following values: 71.4 percent, 72.73%, 70%, 72.73%, and 70% for the positive predictive value, negative predictive value, sensitivity, specificity, and accuracy, respectively. Conclusion: According to this study, PAX8 and CD117 immunohistochemistry expression in ovarian cancer may act as prognostic biomarkers for platinum resistance. In this study there are several limitations that can be revised in future research.https://e-journal.unair.ac.id/MOG/article/view/52881ovarian cancerpax8cd117platinum resistancematernal health |
| spellingShingle | Robby Rinaldi Widodo Tatit Nurseta Edy Mustofa Onni Dwi Arianto R. A. Rose Khasana Dewi The expression of immunohistochemical biomarkers PAX8 and CD117 in platinum resistant ovarian cancer at Saiful Anwar General Hospital, Malang, Indonesia Majalah Obstetri dan Ginekologi ovarian cancer pax8 cd117 platinum resistance maternal health |
| title | The expression of immunohistochemical biomarkers PAX8 and CD117 in platinum resistant ovarian cancer at Saiful Anwar General Hospital, Malang, Indonesia |
| title_full | The expression of immunohistochemical biomarkers PAX8 and CD117 in platinum resistant ovarian cancer at Saiful Anwar General Hospital, Malang, Indonesia |
| title_fullStr | The expression of immunohistochemical biomarkers PAX8 and CD117 in platinum resistant ovarian cancer at Saiful Anwar General Hospital, Malang, Indonesia |
| title_full_unstemmed | The expression of immunohistochemical biomarkers PAX8 and CD117 in platinum resistant ovarian cancer at Saiful Anwar General Hospital, Malang, Indonesia |
| title_short | The expression of immunohistochemical biomarkers PAX8 and CD117 in platinum resistant ovarian cancer at Saiful Anwar General Hospital, Malang, Indonesia |
| title_sort | expression of immunohistochemical biomarkers pax8 and cd117 in platinum resistant ovarian cancer at saiful anwar general hospital malang indonesia |
| topic | ovarian cancer pax8 cd117 platinum resistance maternal health |
| url | https://e-journal.unair.ac.id/MOG/article/view/52881 |
| work_keys_str_mv | AT robbyrinaldiwidodo theexpressionofimmunohistochemicalbiomarkerspax8andcd117inplatinumresistantovariancanceratsaifulanwargeneralhospitalmalangindonesia AT tatitnurseta theexpressionofimmunohistochemicalbiomarkerspax8andcd117inplatinumresistantovariancanceratsaifulanwargeneralhospitalmalangindonesia AT edymustofa theexpressionofimmunohistochemicalbiomarkerspax8andcd117inplatinumresistantovariancanceratsaifulanwargeneralhospitalmalangindonesia AT onnidwiarianto theexpressionofimmunohistochemicalbiomarkerspax8andcd117inplatinumresistantovariancanceratsaifulanwargeneralhospitalmalangindonesia AT rarosekhasanadewi theexpressionofimmunohistochemicalbiomarkerspax8andcd117inplatinumresistantovariancanceratsaifulanwargeneralhospitalmalangindonesia AT robbyrinaldiwidodo expressionofimmunohistochemicalbiomarkerspax8andcd117inplatinumresistantovariancanceratsaifulanwargeneralhospitalmalangindonesia AT tatitnurseta expressionofimmunohistochemicalbiomarkerspax8andcd117inplatinumresistantovariancanceratsaifulanwargeneralhospitalmalangindonesia AT edymustofa expressionofimmunohistochemicalbiomarkerspax8andcd117inplatinumresistantovariancanceratsaifulanwargeneralhospitalmalangindonesia AT onnidwiarianto expressionofimmunohistochemicalbiomarkerspax8andcd117inplatinumresistantovariancanceratsaifulanwargeneralhospitalmalangindonesia AT rarosekhasanadewi expressionofimmunohistochemicalbiomarkerspax8andcd117inplatinumresistantovariancanceratsaifulanwargeneralhospitalmalangindonesia |