Probing host pathogen cross-talk by transcriptional profiling of both Mycobacterium tuberculosis and infected human dendritic cells and macrophages.
<h4>Background</h4>Transcriptional profiling using microarrays provides a unique opportunity to decipher host pathogen cross-talk on the global level. Here, for the first time, we have been able to investigate gene expression changes in both Mycobacterium tuberculosis, a major human path...
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Public Library of Science (PLoS)
2008-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0001403&type=printable |
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| author | Ludovic Tailleux Simon J Waddell Mattia Pelizzola Alessandra Mortellaro Michael Withers Antoine Tanne Paola Ricciardi Castagnoli Brigitte Gicquel Neil G Stoker Philip D Butcher Maria Foti Olivier Neyrolles |
| author_facet | Ludovic Tailleux Simon J Waddell Mattia Pelizzola Alessandra Mortellaro Michael Withers Antoine Tanne Paola Ricciardi Castagnoli Brigitte Gicquel Neil G Stoker Philip D Butcher Maria Foti Olivier Neyrolles |
| author_sort | Ludovic Tailleux |
| collection | DOAJ |
| description | <h4>Background</h4>Transcriptional profiling using microarrays provides a unique opportunity to decipher host pathogen cross-talk on the global level. Here, for the first time, we have been able to investigate gene expression changes in both Mycobacterium tuberculosis, a major human pathogen, and its human host cells, macrophages and dendritic cells.<h4>Methodology/principal findings</h4>In addition to common responses, we could identify eukaryotic and microbial transcriptional signatures that are specific to the cell type involved in the infection process. In particular M. tuberculosis shows a marked stress response when inside dendritic cells, which is in accordance with the low permissivity of these specialized phagocytes to the tubercle bacillus and to other pathogens. In contrast, the mycobacterial transcriptome inside macrophages reflects that of replicating bacteria. On the host cell side, differential responses to infection in macrophages and dendritic cells were identified in genes involved in oxidative stress, intracellular vesicle trafficking and phagosome acidification.<h4>Conclusions/significance</h4>This study provides the proof of principle that probing the host and the microbe transcriptomes simultaneously is a valuable means to accessing unique information on host pathogen interactions. Our results also underline the extraordinary plasticity of host cell and pathogen responses to infection, and provide a solid framework to further understand the complex mechanisms involved in immunity to M. tuberculosis and in mycobacterial adaptation to different intracellular environments. |
| format | Article |
| id | doaj-art-65e48e15cfcc4b5bad153688fefb88e7 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2008-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-65e48e15cfcc4b5bad153688fefb88e72025-08-20T03:22:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-01-0131e140310.1371/journal.pone.0001403Probing host pathogen cross-talk by transcriptional profiling of both Mycobacterium tuberculosis and infected human dendritic cells and macrophages.Ludovic TailleuxSimon J WaddellMattia PelizzolaAlessandra MortellaroMichael WithersAntoine TannePaola Ricciardi CastagnoliBrigitte GicquelNeil G StokerPhilip D ButcherMaria FotiOlivier Neyrolles<h4>Background</h4>Transcriptional profiling using microarrays provides a unique opportunity to decipher host pathogen cross-talk on the global level. Here, for the first time, we have been able to investigate gene expression changes in both Mycobacterium tuberculosis, a major human pathogen, and its human host cells, macrophages and dendritic cells.<h4>Methodology/principal findings</h4>In addition to common responses, we could identify eukaryotic and microbial transcriptional signatures that are specific to the cell type involved in the infection process. In particular M. tuberculosis shows a marked stress response when inside dendritic cells, which is in accordance with the low permissivity of these specialized phagocytes to the tubercle bacillus and to other pathogens. In contrast, the mycobacterial transcriptome inside macrophages reflects that of replicating bacteria. On the host cell side, differential responses to infection in macrophages and dendritic cells were identified in genes involved in oxidative stress, intracellular vesicle trafficking and phagosome acidification.<h4>Conclusions/significance</h4>This study provides the proof of principle that probing the host and the microbe transcriptomes simultaneously is a valuable means to accessing unique information on host pathogen interactions. Our results also underline the extraordinary plasticity of host cell and pathogen responses to infection, and provide a solid framework to further understand the complex mechanisms involved in immunity to M. tuberculosis and in mycobacterial adaptation to different intracellular environments.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0001403&type=printable |
| spellingShingle | Ludovic Tailleux Simon J Waddell Mattia Pelizzola Alessandra Mortellaro Michael Withers Antoine Tanne Paola Ricciardi Castagnoli Brigitte Gicquel Neil G Stoker Philip D Butcher Maria Foti Olivier Neyrolles Probing host pathogen cross-talk by transcriptional profiling of both Mycobacterium tuberculosis and infected human dendritic cells and macrophages. PLoS ONE |
| title | Probing host pathogen cross-talk by transcriptional profiling of both Mycobacterium tuberculosis and infected human dendritic cells and macrophages. |
| title_full | Probing host pathogen cross-talk by transcriptional profiling of both Mycobacterium tuberculosis and infected human dendritic cells and macrophages. |
| title_fullStr | Probing host pathogen cross-talk by transcriptional profiling of both Mycobacterium tuberculosis and infected human dendritic cells and macrophages. |
| title_full_unstemmed | Probing host pathogen cross-talk by transcriptional profiling of both Mycobacterium tuberculosis and infected human dendritic cells and macrophages. |
| title_short | Probing host pathogen cross-talk by transcriptional profiling of both Mycobacterium tuberculosis and infected human dendritic cells and macrophages. |
| title_sort | probing host pathogen cross talk by transcriptional profiling of both mycobacterium tuberculosis and infected human dendritic cells and macrophages |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0001403&type=printable |
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