Experience of Using a Complex Antigenic Preparation of the Plague Microbe to Assess the Severity of a Specific Anti-Plague Response
Background. Improving the methodology of immunological monitoring in natural foci of plague in the Russian Federation and adjacent territories to increase the effectiveness of epidemiological surveillance of plague is an urgent line of research. The lack of correlation between the production of spec...
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| Format: | Article |
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Scientific Сentre for Family Health and Human Reproduction Problems
2021-06-01
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| Series: | Acta Biomedica Scientifica |
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| Online Access: | https://www.actabiomedica.ru/jour/article/view/2727 |
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| author | V. I. Dubrovina K. M. Korytov A. B. Petyatestnikova N. O. Kiseleva V. V. Voitkova D. D. Bryukhova V. B. Nikolaev V. S. Polovinkina L. K. Salchak L. V. Shchuchinov S. V. Balakhonov |
| author_facet | V. I. Dubrovina K. M. Korytov A. B. Petyatestnikova N. O. Kiseleva V. V. Voitkova D. D. Bryukhova V. B. Nikolaev V. S. Polovinkina L. K. Salchak L. V. Shchuchinov S. V. Balakhonov |
| author_sort | V. I. Dubrovina |
| collection | DOAJ |
| description | Background. Improving the methodology of immunological monitoring in natural foci of plague in the Russian Federation and adjacent territories to increase the effectiveness of epidemiological surveillance of plague is an urgent line of research. The lack of correlation between the production of specific antibodies to the capsular antigen (F1) ofthe plague microbe with other indicators of the state of cellular defense reactivity indicates the need to search for new informative and accessible markers for assessing anti-plague immunity.Objective: to evaluate possibility of using the complex preparation (F1 and cell membranes) evaluate the possibilities of using an artificial antigenic complex based on F1 and cell membranes (CM) of the plague microbe in antigen-specific tests in vitro in people vaccinated against plague.resu. The study involved 153 volunteers living in the territory enzootic for plague (the village of Khandagayty ofthe Ovyur kozhuun of the Tyva Republic and the village of Kosh-Agach of the Kosh-Agach district of the Altai Republic). The study included the determination of spontaneous and mitogen-induced production of cytokines (IFN-γ, IL-4, TNF-α) by blood cells, titers of specific IgG antibodies to the capsular antigen F1 of the plague microbe and concentrations ofthe main classes of immunoglobulins (IgM, IgG, IgA and IgE) in blood serum, as well as immunophenotyping of blood lymphocytes (CD3, CD4, CD8, CD16, CD19).Results. Comparative assessment of the level of cytokines (TNF-α, IFN-γ and IL-4) in spontaneous/induced F1+CM Y. pestis tests revealed a statistically significant increase in the production of cytokines TNF-α and IFN-γ in the antigeninduced tests compared with spontaneous (p < 0.01).Conclusion. Thus, the effectiveness of the use of artificial antigenic complex based on F1 and cell membranes ofthe plague microbe has been shown to assess the production of cytokines in antigen-specific cell tests in vitro, which justifies the need for further research. |
| format | Article |
| id | doaj-art-65e25fc7ee8e4f53a996b34072e9c9fa |
| institution | Kabale University |
| issn | 2541-9420 2587-9596 |
| language | Russian |
| publishDate | 2021-06-01 |
| publisher | Scientific Сentre for Family Health and Human Reproduction Problems |
| record_format | Article |
| series | Acta Biomedica Scientifica |
| spelling | doaj-art-65e25fc7ee8e4f53a996b34072e9c9fa2025-08-20T03:56:51ZrusScientific Сentre for Family Health and Human Reproduction ProblemsActa Biomedica Scientifica2541-94202587-95962021-06-0162414610.29413/ABS.2021-6.2.42132Experience of Using a Complex Antigenic Preparation of the Plague Microbe to Assess the Severity of a Specific Anti-Plague ResponseV. I. Dubrovina0K. M. Korytov1A. B. Petyatestnikova2N. O. Kiseleva3V. V. Voitkova4D. D. Bryukhova5V. B. Nikolaev6V. S. Polovinkina7L. K. Salchak8L. V. Shchuchinov9S. V. Balakhonov10Irkutsk Antiplague Research Institute of RospotrebnadzorIrkutsk Antiplague Research Institute of RospotrebnadzorIrkutsk Antiplague Research Institute of RospotrebnadzorIrkutsk Antiplague Research Institute of RospotrebnadzorIrkutsk Antiplague Research Institute of RospotrebnadzorIrkutsk Antiplague Research Institute of RospotrebnadzorIrkutsk Antiplague Research Institute of RospotrebnadzorIrkutsk Antiplague Research Institute of RospotrebnadzorDirectorate of the Rospotrebnadzor for the Republic of TyvaDirectorate of the Rospotrebnadzor for the Republic of AltaiIrkutsk Antiplague Research Institute of RospotrebnadzorBackground. Improving the methodology of immunological monitoring in natural foci of plague in the Russian Federation and adjacent territories to increase the effectiveness of epidemiological surveillance of plague is an urgent line of research. The lack of correlation between the production of specific antibodies to the capsular antigen (F1) ofthe plague microbe with other indicators of the state of cellular defense reactivity indicates the need to search for new informative and accessible markers for assessing anti-plague immunity.Objective: to evaluate possibility of using the complex preparation (F1 and cell membranes) evaluate the possibilities of using an artificial antigenic complex based on F1 and cell membranes (CM) of the plague microbe in antigen-specific tests in vitro in people vaccinated against plague.resu. The study involved 153 volunteers living in the territory enzootic for plague (the village of Khandagayty ofthe Ovyur kozhuun of the Tyva Republic and the village of Kosh-Agach of the Kosh-Agach district of the Altai Republic). The study included the determination of spontaneous and mitogen-induced production of cytokines (IFN-γ, IL-4, TNF-α) by blood cells, titers of specific IgG antibodies to the capsular antigen F1 of the plague microbe and concentrations ofthe main classes of immunoglobulins (IgM, IgG, IgA and IgE) in blood serum, as well as immunophenotyping of blood lymphocytes (CD3, CD4, CD8, CD16, CD19).Results. Comparative assessment of the level of cytokines (TNF-α, IFN-γ and IL-4) in spontaneous/induced F1+CM Y. pestis tests revealed a statistically significant increase in the production of cytokines TNF-α and IFN-γ in the antigeninduced tests compared with spontaneous (p < 0.01).Conclusion. Thus, the effectiveness of the use of artificial antigenic complex based on F1 and cell membranes ofthe plague microbe has been shown to assess the production of cytokines in antigen-specific cell tests in vitro, which justifies the need for further research.https://www.actabiomedica.ru/jour/article/view/2727antigenslymphocyteslive plague vaccinecytokinesimmunity |
| spellingShingle | V. I. Dubrovina K. M. Korytov A. B. Petyatestnikova N. O. Kiseleva V. V. Voitkova D. D. Bryukhova V. B. Nikolaev V. S. Polovinkina L. K. Salchak L. V. Shchuchinov S. V. Balakhonov Experience of Using a Complex Antigenic Preparation of the Plague Microbe to Assess the Severity of a Specific Anti-Plague Response Acta Biomedica Scientifica antigens lymphocytes live plague vaccine cytokines immunity |
| title | Experience of Using a Complex Antigenic Preparation of the Plague Microbe to Assess the Severity of a Specific Anti-Plague Response |
| title_full | Experience of Using a Complex Antigenic Preparation of the Plague Microbe to Assess the Severity of a Specific Anti-Plague Response |
| title_fullStr | Experience of Using a Complex Antigenic Preparation of the Plague Microbe to Assess the Severity of a Specific Anti-Plague Response |
| title_full_unstemmed | Experience of Using a Complex Antigenic Preparation of the Plague Microbe to Assess the Severity of a Specific Anti-Plague Response |
| title_short | Experience of Using a Complex Antigenic Preparation of the Plague Microbe to Assess the Severity of a Specific Anti-Plague Response |
| title_sort | experience of using a complex antigenic preparation of the plague microbe to assess the severity of a specific anti plague response |
| topic | antigens lymphocytes live plague vaccine cytokines immunity |
| url | https://www.actabiomedica.ru/jour/article/view/2727 |
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