β-Cyclodextrin-Modified Laser-Induced Graphene Electrode for Detection of <i>N</i>6-Methyladenosine in RNA
Laser-induced graphene (LIG) possesses characteristics of easy handling, miniaturization, and unique electrical properties. We modified the surface of LIG by electropolymerizing β-cyclodextrin (β-CD), which was used to immobilize antibodies on the electrode surface for highly sensitive detection of...
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MDPI AG
2024-10-01
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| author | Jingyi Guo Mei Zhao Xia Kuang Zilin Chen Fang Wang |
| author_facet | Jingyi Guo Mei Zhao Xia Kuang Zilin Chen Fang Wang |
| author_sort | Jingyi Guo |
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| description | Laser-induced graphene (LIG) possesses characteristics of easy handling, miniaturization, and unique electrical properties. We modified the surface of LIG by electropolymerizing β-cyclodextrin (β-CD), which was used to immobilize antibodies on the electrode surface for highly sensitive detection of targets. <i>N</i>6-methyladenosine (m6A) is the most prevalent reversible modification in mammalian messenger RNA and noncoding RNA, influencing the development of various cancers. Here, β-CD was electropolymerized to immobilize the anti-m6A antibody, which subsequently recognized the target m6A. This was integrated into the catalytic hydrogen peroxide–hydroquinone (H<sub>2</sub>O<sub>2</sub>-HQ) redox system using phos-tag-biotin to generate electrochemical signals from streptavidin-modified horseradish peroxidase (SA-HRP). Under optimal conditions, the biosensor exhibited a linear range from 0.1 to 100 nM with a minimum detection limit of 96 pM. The method was successfully applied to the recovery analysis of m6A from HeLa cells through spiking experiments and aims to inspire strategies for point-of-care testing (POCT). |
| format | Article |
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| institution | OA Journals |
| issn | 1420-3049 |
| language | English |
| publishDate | 2024-10-01 |
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| series | Molecules |
| spelling | doaj-art-65d7d3ef0bfd4b4691e84afffc17fc282025-08-20T02:16:55ZengMDPI AGMolecules1420-30492024-10-012919471810.3390/molecules29194718β-Cyclodextrin-Modified Laser-Induced Graphene Electrode for Detection of <i>N</i>6-Methyladenosine in RNAJingyi Guo0Mei Zhao1Xia Kuang2Zilin Chen3Fang Wang4Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (MOE), School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, ChinaKey Laboratory of Combinatorial Biosynthesis and Drug Discovery (MOE), School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, ChinaKey Laboratory of Combinatorial Biosynthesis and Drug Discovery (MOE), School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, ChinaKey Laboratory of Combinatorial Biosynthesis and Drug Discovery (MOE), School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, ChinaKey Laboratory of Combinatorial Biosynthesis and Drug Discovery (MOE), School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, ChinaLaser-induced graphene (LIG) possesses characteristics of easy handling, miniaturization, and unique electrical properties. We modified the surface of LIG by electropolymerizing β-cyclodextrin (β-CD), which was used to immobilize antibodies on the electrode surface for highly sensitive detection of targets. <i>N</i>6-methyladenosine (m6A) is the most prevalent reversible modification in mammalian messenger RNA and noncoding RNA, influencing the development of various cancers. Here, β-CD was electropolymerized to immobilize the anti-m6A antibody, which subsequently recognized the target m6A. This was integrated into the catalytic hydrogen peroxide–hydroquinone (H<sub>2</sub>O<sub>2</sub>-HQ) redox system using phos-tag-biotin to generate electrochemical signals from streptavidin-modified horseradish peroxidase (SA-HRP). Under optimal conditions, the biosensor exhibited a linear range from 0.1 to 100 nM with a minimum detection limit of 96 pM. The method was successfully applied to the recovery analysis of m6A from HeLa cells through spiking experiments and aims to inspire strategies for point-of-care testing (POCT).https://www.mdpi.com/1420-3049/29/19/4718electrochemical biosensor<i>N</i>6-methyladenosinelaser-induced graphenenucleic acid methylation |
| spellingShingle | Jingyi Guo Mei Zhao Xia Kuang Zilin Chen Fang Wang β-Cyclodextrin-Modified Laser-Induced Graphene Electrode for Detection of <i>N</i>6-Methyladenosine in RNA Molecules electrochemical biosensor <i>N</i>6-methyladenosine laser-induced graphene nucleic acid methylation |
| title | β-Cyclodextrin-Modified Laser-Induced Graphene Electrode for Detection of <i>N</i>6-Methyladenosine in RNA |
| title_full | β-Cyclodextrin-Modified Laser-Induced Graphene Electrode for Detection of <i>N</i>6-Methyladenosine in RNA |
| title_fullStr | β-Cyclodextrin-Modified Laser-Induced Graphene Electrode for Detection of <i>N</i>6-Methyladenosine in RNA |
| title_full_unstemmed | β-Cyclodextrin-Modified Laser-Induced Graphene Electrode for Detection of <i>N</i>6-Methyladenosine in RNA |
| title_short | β-Cyclodextrin-Modified Laser-Induced Graphene Electrode for Detection of <i>N</i>6-Methyladenosine in RNA |
| title_sort | β cyclodextrin modified laser induced graphene electrode for detection of i n i 6 methyladenosine in rna |
| topic | electrochemical biosensor <i>N</i>6-methyladenosine laser-induced graphene nucleic acid methylation |
| url | https://www.mdpi.com/1420-3049/29/19/4718 |
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