Genomic analysis of oxacillin-susceptible mecA-positive Staphylococcus haemolyticus ST159 isolated from anovaginal colonization in Brazil
Oxacillin-susceptible methicillin-resistant Staphylococcus spp. (OS-MRS) is a known phenotype, mainly regarding S. aureus. It is considered a concerning pathogen due to its difficulty to be detected only with phenotypic methods, and reporting false susceptibility to beta-lactams could lead ineffecti...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-03-01
|
| Series: | International Journal of Infectious Diseases |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971224006805 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850240836785143808 |
|---|---|
| author | Andre Rio Tinto Dr Natáli Costa Dr Laura Oliveira Prof. Sergio Fracalanzza Prof. Lucia Teixeira Dr. Penelope Marinho Prof. Tatiana Pinto |
| author_facet | Andre Rio Tinto Dr Natáli Costa Dr Laura Oliveira Prof. Sergio Fracalanzza Prof. Lucia Teixeira Dr. Penelope Marinho Prof. Tatiana Pinto |
| author_sort | Andre Rio Tinto |
| collection | DOAJ |
| description | Oxacillin-susceptible methicillin-resistant Staphylococcus spp. (OS-MRS) is a known phenotype, mainly regarding S. aureus. It is considered a concerning pathogen due to its difficulty to be detected only with phenotypic methods, and reporting false susceptibility to beta-lactams could lead ineffective treatment. During a surveillance study for detection of anovaginal colonization of methicillin-resistant Staphylococcus spp. (MRS) among pregnant population, between April 2021 and August 2022, 518 anovaginal specimens were streaked onto CHROMagarTM MRSA, and 331 strains were obtained. Phenotypic confirmation for methicillin resistance was performed using cefoxitin disc diffusion, according to CLSI, and 27 (8.2%) were susceptible. PCR detection for mecA was performed for all strains, and 11 (3.3%) phenotypic susceptible strains harbored the mecA gene, hence belonging to the OS-MRS phenotype. One OS-MR S. haemolyticus strain was randomly selected for whole genome sequencing, using Illumina. Genome analysis using CARD and ResFinder database confirmed the presence of the mecA gene, along with other resistance determinants: APH(3′)-IIIa, blaZ, mgrA, mphC, msrA, and sat4. Sequence alignment with reference sequences of all SCCmec types was performed using BLAST and indicated as best results 100% identity and only 55 and 52% query coverage with SCCmec types VI and IVc respectively. A novel ST159 was determined using PubMLST. This data corroborates the need for further understanding of OS-MRS epidemiology in our setting, and the importance of specific protocols for its detection. |
| format | Article |
| id | doaj-art-65d032ed034a4b8dae45e2f130987b23 |
| institution | OA Journals |
| issn | 1201-9712 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj-art-65d032ed034a4b8dae45e2f130987b232025-08-20T02:00:46ZengElsevierInternational Journal of Infectious Diseases1201-97122025-03-0115210760510.1016/j.ijid.2024.107605Genomic analysis of oxacillin-susceptible mecA-positive Staphylococcus haemolyticus ST159 isolated from anovaginal colonization in BrazilAndre Rio Tinto0Dr Natáli Costa1Dr Laura Oliveira2Prof. Sergio Fracalanzza3Prof. Lucia Teixeira4Dr. Penelope Marinho5Prof. Tatiana Pinto6Universidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroMaternidade Escola da Universidade Federal do Rio de JaneiroUniversidade Federal do Rio de JaneiroOxacillin-susceptible methicillin-resistant Staphylococcus spp. (OS-MRS) is a known phenotype, mainly regarding S. aureus. It is considered a concerning pathogen due to its difficulty to be detected only with phenotypic methods, and reporting false susceptibility to beta-lactams could lead ineffective treatment. During a surveillance study for detection of anovaginal colonization of methicillin-resistant Staphylococcus spp. (MRS) among pregnant population, between April 2021 and August 2022, 518 anovaginal specimens were streaked onto CHROMagarTM MRSA, and 331 strains were obtained. Phenotypic confirmation for methicillin resistance was performed using cefoxitin disc diffusion, according to CLSI, and 27 (8.2%) were susceptible. PCR detection for mecA was performed for all strains, and 11 (3.3%) phenotypic susceptible strains harbored the mecA gene, hence belonging to the OS-MRS phenotype. One OS-MR S. haemolyticus strain was randomly selected for whole genome sequencing, using Illumina. Genome analysis using CARD and ResFinder database confirmed the presence of the mecA gene, along with other resistance determinants: APH(3′)-IIIa, blaZ, mgrA, mphC, msrA, and sat4. Sequence alignment with reference sequences of all SCCmec types was performed using BLAST and indicated as best results 100% identity and only 55 and 52% query coverage with SCCmec types VI and IVc respectively. A novel ST159 was determined using PubMLST. This data corroborates the need for further understanding of OS-MRS epidemiology in our setting, and the importance of specific protocols for its detection.http://www.sciencedirect.com/science/article/pii/S1201971224006805 |
| spellingShingle | Andre Rio Tinto Dr Natáli Costa Dr Laura Oliveira Prof. Sergio Fracalanzza Prof. Lucia Teixeira Dr. Penelope Marinho Prof. Tatiana Pinto Genomic analysis of oxacillin-susceptible mecA-positive Staphylococcus haemolyticus ST159 isolated from anovaginal colonization in Brazil International Journal of Infectious Diseases |
| title | Genomic analysis of oxacillin-susceptible mecA-positive Staphylococcus haemolyticus ST159 isolated from anovaginal colonization in Brazil |
| title_full | Genomic analysis of oxacillin-susceptible mecA-positive Staphylococcus haemolyticus ST159 isolated from anovaginal colonization in Brazil |
| title_fullStr | Genomic analysis of oxacillin-susceptible mecA-positive Staphylococcus haemolyticus ST159 isolated from anovaginal colonization in Brazil |
| title_full_unstemmed | Genomic analysis of oxacillin-susceptible mecA-positive Staphylococcus haemolyticus ST159 isolated from anovaginal colonization in Brazil |
| title_short | Genomic analysis of oxacillin-susceptible mecA-positive Staphylococcus haemolyticus ST159 isolated from anovaginal colonization in Brazil |
| title_sort | genomic analysis of oxacillin susceptible meca positive staphylococcus haemolyticus st159 isolated from anovaginal colonization in brazil |
| url | http://www.sciencedirect.com/science/article/pii/S1201971224006805 |
| work_keys_str_mv | AT andreriotinto genomicanalysisofoxacillinsusceptiblemecapositivestaphylococcushaemolyticusst159isolatedfromanovaginalcolonizationinbrazil AT drnatalicosta genomicanalysisofoxacillinsusceptiblemecapositivestaphylococcushaemolyticusst159isolatedfromanovaginalcolonizationinbrazil AT drlauraoliveira genomicanalysisofoxacillinsusceptiblemecapositivestaphylococcushaemolyticusst159isolatedfromanovaginalcolonizationinbrazil AT profsergiofracalanzza genomicanalysisofoxacillinsusceptiblemecapositivestaphylococcushaemolyticusst159isolatedfromanovaginalcolonizationinbrazil AT profluciateixeira genomicanalysisofoxacillinsusceptiblemecapositivestaphylococcushaemolyticusst159isolatedfromanovaginalcolonizationinbrazil AT drpenelopemarinho genomicanalysisofoxacillinsusceptiblemecapositivestaphylococcushaemolyticusst159isolatedfromanovaginalcolonizationinbrazil AT proftatianapinto genomicanalysisofoxacillinsusceptiblemecapositivestaphylococcushaemolyticusst159isolatedfromanovaginalcolonizationinbrazil |