Kodamaea ohmeri: An emergent yeast from a One Health perspective

Kodamaea ohmeri is an emerging and opportunistic yeast associated with a high mortality rate in humans. As it is commonly found in the environment, it is possible that environmental conditions and agricultural practices contribute to the adaptation of this yeast and the selection of antifungal resis...

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Main Authors: Sthefany Emanuelle Silva, Lorena Souza Silva, Ludmila Gouveia Eufrasio, Gabriela Silva Cruz, Fabíola Lucini, Hareton Teixeira Vechi, Manoella do Monte Alves, Luciana Rodrigues Ferreira Ribeiro, Karine Lilian de Souza, José Aparecido Moreira, Janete Gouveia de Souza, Florent Morio, Gisela Lara da Costa, Barbara de Oliveira Baptista, Luiz Marcelo Ribeiro Tomé, Sílvia Helena Sousa Pietra Pedroso, Felipe Campos de Melo Iani, Talita Émile Ribeiro Adelino, Débora Castelo-Branco, Luana Rossato, Nalu Teixeira de Aguiar Peres, Daniel Assis Santos, Manoel Marques Evangelista Oliveira, Kássia Jéssica Galdino da Silva, Rafael Wesley Bastos
Format: Article
Language:English
Published: Elsevier 2025-01-01
Series:Current Research in Microbial Sciences
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666517425000215
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author Sthefany Emanuelle Silva
Lorena Souza Silva
Ludmila Gouveia Eufrasio
Gabriela Silva Cruz
Fabíola Lucini
Hareton Teixeira Vechi
Manoella do Monte Alves
Luciana Rodrigues Ferreira Ribeiro
Karine Lilian de Souza
José Aparecido Moreira
Janete Gouveia de Souza
Florent Morio
Gisela Lara da Costa
Barbara de Oliveira Baptista
Luiz Marcelo Ribeiro Tomé
Sílvia Helena Sousa Pietra Pedroso
Felipe Campos de Melo Iani
Talita Émile Ribeiro Adelino
Débora Castelo-Branco
Luana Rossato
Nalu Teixeira de Aguiar Peres
Daniel Assis Santos
Manoel Marques Evangelista Oliveira
Kássia Jéssica Galdino da Silva
Rafael Wesley Bastos
author_facet Sthefany Emanuelle Silva
Lorena Souza Silva
Ludmila Gouveia Eufrasio
Gabriela Silva Cruz
Fabíola Lucini
Hareton Teixeira Vechi
Manoella do Monte Alves
Luciana Rodrigues Ferreira Ribeiro
Karine Lilian de Souza
José Aparecido Moreira
Janete Gouveia de Souza
Florent Morio
Gisela Lara da Costa
Barbara de Oliveira Baptista
Luiz Marcelo Ribeiro Tomé
Sílvia Helena Sousa Pietra Pedroso
Felipe Campos de Melo Iani
Talita Émile Ribeiro Adelino
Débora Castelo-Branco
Luana Rossato
Nalu Teixeira de Aguiar Peres
Daniel Assis Santos
Manoel Marques Evangelista Oliveira
Kássia Jéssica Galdino da Silva
Rafael Wesley Bastos
author_sort Sthefany Emanuelle Silva
collection DOAJ
description Kodamaea ohmeri is an emerging and opportunistic yeast associated with a high mortality rate in humans. As it is commonly found in the environment, it is possible that environmental conditions and agricultural practices contribute to the adaptation of this yeast and the selection of antifungal resistance. During a multicentric study in Brazil, conducted under a One Health perspective, 14 isolates of K. ohmeri were identified from different sources: three from blood cultures, three from animals (swine and poultry), and eight from animal environments (swine and poultry). Yeasts were isolated using CHROmagar® Candida medium and identified by MALDI-TOF MS and ITS rDNA barcoding. Minimum inhibitory concentration (MIC) was determined using the broth microdilution method for clinical (azoles, echinocandins, pyrimidine analogs, and polyenes), and environmental antifungals (tebuconazole, pyraclostrobin, carbendazim, and mancozeb), and hospital disinfectants (quaternary ammonium compounds). Of note, color variations of K. ohmeri were noted on CHROmagar® depending on the incubation time, which is likely to complicate its identification. Following polyphasic identification and taxonomic confirmation, all isolates demonstrated low MIC values for clinical antifungals, disinfectants, and tebuconazole. However, all isolates were able to grow in the presence of carbendazim, mancozeb, and pyraclostrobin. Together, these findings highlight the risks associated with the use of environmental azoles, such as tebuconazole, as they may impact non-target fungi of medical importance, but other fungicides do not present the same risk. This is the first study to demonstrate that K. ohmeri, an important emerging yeast in human medicine, can be isolated from various sources, including patients. Although the isolates exhibited low MIC values for clinical antifungals, it is crucial to monitor changes in sensitivity patterns over time in emerging microorganisms to prevent the development of multidrug resistance, which may originate in the environment.
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spelling doaj-art-65bf046d36c7487e9d0764040f78e4a92025-08-20T02:35:45ZengElsevierCurrent Research in Microbial Sciences2666-51742025-01-01810035910.1016/j.crmicr.2025.100359Kodamaea ohmeri: An emergent yeast from a One Health perspectiveSthefany Emanuelle Silva0Lorena Souza Silva1Ludmila Gouveia Eufrasio2Gabriela Silva Cruz3Fabíola Lucini4Hareton Teixeira Vechi5Manoella do Monte Alves6Luciana Rodrigues Ferreira Ribeiro7Karine Lilian de Souza8José Aparecido Moreira9Janete Gouveia de Souza10Florent Morio11Gisela Lara da Costa12Barbara de Oliveira Baptista13Luiz Marcelo Ribeiro Tomé14Sílvia Helena Sousa Pietra Pedroso15Felipe Campos de Melo Iani16Talita Émile Ribeiro Adelino17Débora Castelo-Branco18Luana Rossato19Nalu Teixeira de Aguiar Peres20Daniel Assis Santos21Manoel Marques Evangelista Oliveira22Kássia Jéssica Galdino da Silva23Rafael Wesley Bastos24Departamento de Microbiologia e Parasitologia, Laboratório de Uso Comum, Centro de Biociências, Universidade Federal do Rio Grande do Norte, Campus Universitário UFRN, Bloco D1, Av. Sen. Salgado Filho, 3000 - Lagoa Nova, Natal, RN 59064-741, BrazilDepartamento de Microbiologia e Parasitologia, Laboratório de Uso Comum, Centro de Biociências, Universidade Federal do Rio Grande do Norte, Campus Universitário UFRN, Bloco D1, Av. Sen. Salgado Filho, 3000 - Lagoa Nova, Natal, RN 59064-741, BrazilDepartamento de Microbiologia, Universidade Federal de Minas Gerais, BrazilDepartamento de Patologia e Medicina Legal, Universidade Federal do Ceará, BrazilLaboratório de Pesquisa em Ciências da Saúde, Universidade Federal da Grande Dourados, BrazilInstituto de Medicina Tropical do Rio Grande do Norte, Universidade Federal do Rio Grande do Norte, Brazil; Escola Multicampi de Ciências Médicas, Universidade Federal do Rio Grande do Norte, Brazil; Hospital do Coração, Rio Grande do Norte, BrazilDepartamento de Infectologia, Universidade Federal do Rio Grande do Norte, Brazil; Hospital Giselda Trigueiro, Natal, Rio Grande do Norte, BrazilHospital Giselda Trigueiro, Natal, Rio Grande do Norte, BrazilHospital do Coração, Rio Grande do Norte, BrazilEscola Agrícola de Jundiaí, Universidade Federal do Rio Grande do Norte, BrazilEscola Agrícola de Jundiaí, Universidade Federal do Rio Grande do Norte, BrazilCHU Nantes, Cibles et Médicaments des Infections et de l'Immunité, UR1155, Nantes Université, Nantes, FranceLaboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos, Fundação Oswaldo Cruz, BrazilLaboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos, Fundação Oswaldo Cruz, BrazilFundação Ezequiel Dias – Belo Horizonte, Minas Gerais, BrazilFundação Ezequiel Dias – Belo Horizonte, Minas Gerais, BrazilFundação Ezequiel Dias – Belo Horizonte, Minas Gerais, BrazilFundação Ezequiel Dias – Belo Horizonte, Minas Gerais, BrazilDepartamento de Patologia e Medicina Legal, Universidade Federal do Ceará, BrazilLaboratório de Pesquisa em Ciências da Saúde, Universidade Federal da Grande Dourados, BrazilDepartamento de Microbiologia, Universidade Federal de Minas Gerais, BrazilDepartamento de Microbiologia, Universidade Federal de Minas Gerais, BrazilLaboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos, Fundação Oswaldo Cruz, BrazilDepartamento de Microbiologia e Parasitologia, Laboratório de Uso Comum, Centro de Biociências, Universidade Federal do Rio Grande do Norte, Campus Universitário UFRN, Bloco D1, Av. Sen. Salgado Filho, 3000 - Lagoa Nova, Natal, RN 59064-741, BrazilDepartamento de Microbiologia e Parasitologia, Laboratório de Uso Comum, Centro de Biociências, Universidade Federal do Rio Grande do Norte, Campus Universitário UFRN, Bloco D1, Av. Sen. Salgado Filho, 3000 - Lagoa Nova, Natal, RN 59064-741, Brazil; Corresponding author.Kodamaea ohmeri is an emerging and opportunistic yeast associated with a high mortality rate in humans. As it is commonly found in the environment, it is possible that environmental conditions and agricultural practices contribute to the adaptation of this yeast and the selection of antifungal resistance. During a multicentric study in Brazil, conducted under a One Health perspective, 14 isolates of K. ohmeri were identified from different sources: three from blood cultures, three from animals (swine and poultry), and eight from animal environments (swine and poultry). Yeasts were isolated using CHROmagar® Candida medium and identified by MALDI-TOF MS and ITS rDNA barcoding. Minimum inhibitory concentration (MIC) was determined using the broth microdilution method for clinical (azoles, echinocandins, pyrimidine analogs, and polyenes), and environmental antifungals (tebuconazole, pyraclostrobin, carbendazim, and mancozeb), and hospital disinfectants (quaternary ammonium compounds). Of note, color variations of K. ohmeri were noted on CHROmagar® depending on the incubation time, which is likely to complicate its identification. Following polyphasic identification and taxonomic confirmation, all isolates demonstrated low MIC values for clinical antifungals, disinfectants, and tebuconazole. However, all isolates were able to grow in the presence of carbendazim, mancozeb, and pyraclostrobin. Together, these findings highlight the risks associated with the use of environmental azoles, such as tebuconazole, as they may impact non-target fungi of medical importance, but other fungicides do not present the same risk. This is the first study to demonstrate that K. ohmeri, an important emerging yeast in human medicine, can be isolated from various sources, including patients. Although the isolates exhibited low MIC values for clinical antifungals, it is crucial to monitor changes in sensitivity patterns over time in emerging microorganisms to prevent the development of multidrug resistance, which may originate in the environment.http://www.sciencedirect.com/science/article/pii/S2666517425000215AgrochemicalsHospital disinfectantsYeastsCross-resistance
spellingShingle Sthefany Emanuelle Silva
Lorena Souza Silva
Ludmila Gouveia Eufrasio
Gabriela Silva Cruz
Fabíola Lucini
Hareton Teixeira Vechi
Manoella do Monte Alves
Luciana Rodrigues Ferreira Ribeiro
Karine Lilian de Souza
José Aparecido Moreira
Janete Gouveia de Souza
Florent Morio
Gisela Lara da Costa
Barbara de Oliveira Baptista
Luiz Marcelo Ribeiro Tomé
Sílvia Helena Sousa Pietra Pedroso
Felipe Campos de Melo Iani
Talita Émile Ribeiro Adelino
Débora Castelo-Branco
Luana Rossato
Nalu Teixeira de Aguiar Peres
Daniel Assis Santos
Manoel Marques Evangelista Oliveira
Kássia Jéssica Galdino da Silva
Rafael Wesley Bastos
Kodamaea ohmeri: An emergent yeast from a One Health perspective
Current Research in Microbial Sciences
Agrochemicals
Hospital disinfectants
Yeasts
Cross-resistance
title Kodamaea ohmeri: An emergent yeast from a One Health perspective
title_full Kodamaea ohmeri: An emergent yeast from a One Health perspective
title_fullStr Kodamaea ohmeri: An emergent yeast from a One Health perspective
title_full_unstemmed Kodamaea ohmeri: An emergent yeast from a One Health perspective
title_short Kodamaea ohmeri: An emergent yeast from a One Health perspective
title_sort kodamaea ohmeri an emergent yeast from a one health perspective
topic Agrochemicals
Hospital disinfectants
Yeasts
Cross-resistance
url http://www.sciencedirect.com/science/article/pii/S2666517425000215
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