Determination of the New SNP g.939A>G of the TYR Gene in Abnormal Coat Color in Bali Cattle

This study aimed to assess the presence of single nucleotide polymorphisms (SNPs) in the exon 1 region of the tyrosinase (TYR) gene in Bali cattle with abnormal coat color. This study analyzed 43 Bali cattle, 26 individuals with typical or standard coat color, and 17 individuals with albinism. The g...

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Main Authors: Kholijah, Mokhmad Fakhrul Ulum, Sri Darwati, Ronny Rachman Noor, Jakaria
Format: Article
Language:English
Published: Fakultas Peternakan Universitas Brawijaya 2024-04-01
Series:Jurnal Ilmu-Ilmu Peternakan
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Online Access:https://jiip.ub.ac.id/index.php/jiip/article/view/2866
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author Kholijah
Mokhmad Fakhrul Ulum
Sri Darwati
Ronny Rachman Noor
Jakaria
author_facet Kholijah
Mokhmad Fakhrul Ulum
Sri Darwati
Ronny Rachman Noor
Jakaria
author_sort Kholijah
collection DOAJ
description This study aimed to assess the presence of single nucleotide polymorphisms (SNPs) in the exon 1 region of the tyrosinase (TYR) gene in Bali cattle with abnormal coat color. This study analyzed 43 Bali cattle, 26 individuals with typical or standard coat color, and 17 individuals with albinism. The genetic diversity of the TYR gene was determined via direct sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) techniques. The sequence data of the TYR gene were scrutinized using BioEdit and MEGA10 software to detect single nucleotide polymorphisms (SNPs) related to different types of coat color features in Bali cattle. The genetic diversity information was derived from computations performed using PopGen 1.32 software. The results showed that exon 1 of the TYR gene was affected by the new SNP g.939A>G, which is polymorphic in Bali cattle. In conclusion, the SNP c.939A>G can be further analyzed for use as a candidate-assisted selection (MAS) for abnormal coat color in Bali cattle.
format Article
id doaj-art-65a23cb93cf3479da5185ddcf0fb8313
institution Kabale University
issn 0852-3681
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language English
publishDate 2024-04-01
publisher Fakultas Peternakan Universitas Brawijaya
record_format Article
series Jurnal Ilmu-Ilmu Peternakan
spelling doaj-art-65a23cb93cf3479da5185ddcf0fb83132025-08-20T03:33:42ZengFakultas Peternakan Universitas BrawijayaJurnal Ilmu-Ilmu Peternakan0852-36812443-07652024-04-01341435010.21776/ub.jiip.2024.034.01.054295Determination of the New SNP g.939A>G of the TYR Gene in Abnormal Coat Color in Bali CattleKholijah0https://orcid.org/0009-0001-0700-6750Mokhmad Fakhrul Ulum1https://orcid.org/0000-0003-2092-9645Sri Darwati2https://orcid.org/0000-0002-4882-3722Ronny Rachman Noor3Jakaria4https://orcid.org/0000-0001-7921-8528Department of Animal Production and Technology, Faculty of Animal Science, IPB University, Bogor, 16680, Indonesia.Division of Reproduction and Obstetrics, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, 16680, Indonesia.Department of Animal Production and Technology, Faculty of Animal Science, IPB University, Bogor, 16680, Indonesia.Department of Animal Production and Technology, Faculty of Animal Science, IPB University, Bogor, 16680, Indonesia.Department of Animal Production and Technology, Faculty of Animal Science, IPB University, Bogor, 16680, Indonesia.This study aimed to assess the presence of single nucleotide polymorphisms (SNPs) in the exon 1 region of the tyrosinase (TYR) gene in Bali cattle with abnormal coat color. This study analyzed 43 Bali cattle, 26 individuals with typical or standard coat color, and 17 individuals with albinism. The genetic diversity of the TYR gene was determined via direct sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) techniques. The sequence data of the TYR gene were scrutinized using BioEdit and MEGA10 software to detect single nucleotide polymorphisms (SNPs) related to different types of coat color features in Bali cattle. The genetic diversity information was derived from computations performed using PopGen 1.32 software. The results showed that exon 1 of the TYR gene was affected by the new SNP g.939A>G, which is polymorphic in Bali cattle. In conclusion, the SNP c.939A>G can be further analyzed for use as a candidate-assisted selection (MAS) for abnormal coat color in Bali cattle.https://jiip.ub.ac.id/index.php/jiip/article/view/2866bali cattlecoat colorsnptyr gene
spellingShingle Kholijah
Mokhmad Fakhrul Ulum
Sri Darwati
Ronny Rachman Noor
Jakaria
Determination of the New SNP g.939A>G of the TYR Gene in Abnormal Coat Color in Bali Cattle
Jurnal Ilmu-Ilmu Peternakan
bali cattle
coat color
snp
tyr gene
title Determination of the New SNP g.939A>G of the TYR Gene in Abnormal Coat Color in Bali Cattle
title_full Determination of the New SNP g.939A>G of the TYR Gene in Abnormal Coat Color in Bali Cattle
title_fullStr Determination of the New SNP g.939A>G of the TYR Gene in Abnormal Coat Color in Bali Cattle
title_full_unstemmed Determination of the New SNP g.939A>G of the TYR Gene in Abnormal Coat Color in Bali Cattle
title_short Determination of the New SNP g.939A>G of the TYR Gene in Abnormal Coat Color in Bali Cattle
title_sort determination of the new snp g 939a g of the tyr gene in abnormal coat color in bali cattle
topic bali cattle
coat color
snp
tyr gene
url https://jiip.ub.ac.id/index.php/jiip/article/view/2866
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AT mokhmadfakhrululum determinationofthenewsnpg939agofthetyrgeneinabnormalcoatcolorinbalicattle
AT sridarwati determinationofthenewsnpg939agofthetyrgeneinabnormalcoatcolorinbalicattle
AT ronnyrachmannoor determinationofthenewsnpg939agofthetyrgeneinabnormalcoatcolorinbalicattle
AT jakaria determinationofthenewsnpg939agofthetyrgeneinabnormalcoatcolorinbalicattle