Standardization of indirect regeneration protocol in Lady Rosetta cultivar of potato (Solanum tuberosum)
The present experiment was conducted during 2021–22 at School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, Punjab to standardize indirect somatic embryogenesis for rapid callus induction, plantlet regeneration and shoot proliferation in the popular potato (Solanum tubero...
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Indian Council of Agricultural Research
2025-06-01
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| Series: | The Indian Journal of Agricultural Sciences |
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| Online Access: | https://epubs.icar.org.in/index.php/IJAgS/article/view/163276 |
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| author | LAKSHYA SAXENA POOJA MANCHANDA MANINDER KAUR ANU KALIA SAT PAL SHARMA |
| author_facet | LAKSHYA SAXENA POOJA MANCHANDA MANINDER KAUR ANU KALIA SAT PAL SHARMA |
| author_sort | LAKSHYA SAXENA |
| collection | DOAJ |
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The present experiment was conducted during 2021–22 at School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, Punjab to standardize indirect somatic embryogenesis for rapid callus induction, plantlet regeneration and shoot proliferation in the popular potato (Solanum tuberosum L.) cv. Lady Rosetta. Nodal and internodal regions were used as explants for the process under sterilized laminar air flow cabinet conditions. The experiment was laid out in a completely randomized design (CRD) with three replications. About 9.78% callus induction were noticed in the explants cultured on Murashige and Skoog (MS) media supplemented with growth regulators i.e 4.0 mg/L 2, 4-Dichlorophenoxy acetic acid (2,4-D) and 0.5 mg/L Kinetin (Kin) for induction of callus and regeneration of shoot tissues from callus. Simultaneously, 48.9% plantlet regeneration from calli was obtained in the MS media with 4 mg/L Benzyl Amino Purine (BAP) and 1 mg/L Kin. We observed better callus induction at 4.0 mg/L 2,4-D and 0.5 mg/L Kin, shoot induction at 4.0 mg/L BAP and 1.0 mg/L Kin and root induction in half-strength MS media. The regenerated shoots were efficiently grown on rooting media, later transferred for hardening and acclimatized under field conditions. The present study serves as an efficient protocol to conduct tissue culture experiment using potato explants for calli induction, shoot regeneration and rooting in cv. Lady Rosetta. These findings will serve as a significant finding for regenerating potato plants from calli for genetic transformation/genome editing studies.
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| format | Article |
| id | doaj-art-659a75bbc25b4d3d8430b56c7025428e |
| institution | Kabale University |
| issn | 0019-5022 2394-3319 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | The Indian Journal of Agricultural Sciences |
| spelling | doaj-art-659a75bbc25b4d3d8430b56c7025428e2025-08-20T03:44:28ZengIndian Council of Agricultural ResearchThe Indian Journal of Agricultural Sciences0019-50222394-33192025-06-0195610.56093/ijas.v95i6.163276Standardization of indirect regeneration protocol in Lady Rosetta cultivar of potato (Solanum tuberosum)LAKSHYA SAXENA0POOJA MANCHANDA1MANINDER KAUR2ANU KALIA3SAT PAL SHARMA4School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, PunjabSchool of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, Punjabchool of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, Punjab 141 027, IndiaPunjab Agricultural University, Ludhiana, PunjabPunjab Agricultural University, Ludhiana, Punjab The present experiment was conducted during 2021–22 at School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, Punjab to standardize indirect somatic embryogenesis for rapid callus induction, plantlet regeneration and shoot proliferation in the popular potato (Solanum tuberosum L.) cv. Lady Rosetta. Nodal and internodal regions were used as explants for the process under sterilized laminar air flow cabinet conditions. The experiment was laid out in a completely randomized design (CRD) with three replications. About 9.78% callus induction were noticed in the explants cultured on Murashige and Skoog (MS) media supplemented with growth regulators i.e 4.0 mg/L 2, 4-Dichlorophenoxy acetic acid (2,4-D) and 0.5 mg/L Kinetin (Kin) for induction of callus and regeneration of shoot tissues from callus. Simultaneously, 48.9% plantlet regeneration from calli was obtained in the MS media with 4 mg/L Benzyl Amino Purine (BAP) and 1 mg/L Kin. We observed better callus induction at 4.0 mg/L 2,4-D and 0.5 mg/L Kin, shoot induction at 4.0 mg/L BAP and 1.0 mg/L Kin and root induction in half-strength MS media. The regenerated shoots were efficiently grown on rooting media, later transferred for hardening and acclimatized under field conditions. The present study serves as an efficient protocol to conduct tissue culture experiment using potato explants for calli induction, shoot regeneration and rooting in cv. Lady Rosetta. These findings will serve as a significant finding for regenerating potato plants from calli for genetic transformation/genome editing studies. https://epubs.icar.org.in/index.php/IJAgS/article/view/163276Callus, Hardening, Internode, Potato, Tissue culture |
| spellingShingle | LAKSHYA SAXENA POOJA MANCHANDA MANINDER KAUR ANU KALIA SAT PAL SHARMA Standardization of indirect regeneration protocol in Lady Rosetta cultivar of potato (Solanum tuberosum) The Indian Journal of Agricultural Sciences Callus, Hardening, Internode, Potato, Tissue culture |
| title | Standardization of indirect regeneration protocol in Lady Rosetta cultivar of potato (Solanum tuberosum) |
| title_full | Standardization of indirect regeneration protocol in Lady Rosetta cultivar of potato (Solanum tuberosum) |
| title_fullStr | Standardization of indirect regeneration protocol in Lady Rosetta cultivar of potato (Solanum tuberosum) |
| title_full_unstemmed | Standardization of indirect regeneration protocol in Lady Rosetta cultivar of potato (Solanum tuberosum) |
| title_short | Standardization of indirect regeneration protocol in Lady Rosetta cultivar of potato (Solanum tuberosum) |
| title_sort | standardization of indirect regeneration protocol in lady rosetta cultivar of potato solanum tuberosum |
| topic | Callus, Hardening, Internode, Potato, Tissue culture |
| url | https://epubs.icar.org.in/index.php/IJAgS/article/view/163276 |
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