Effects of Ethyl Pyruvate on Bile Duct Ligation-Induced Liver Fibrosis by Regulating Nrf2 Pathway and Proinflammatory Cytokines in Rats
Aim. The aim of this paper is to investigate the effects of ethyl pyruvate (EP) on experimental liver fibrosis induced by bile duct ligation (BDL) and explore the underlying molecular mechanisms. Material and Method. Rats were randomly divided into three groups: the sham group, the BDL group, and th...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2019/2969802 |
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| author | Yonghua Zong Mingxiao Zhang Shuai Li Wenqian Qi Juan Li Tonghua Liu Huijun Yang Chen Lu Xiaosong Hu |
| author_facet | Yonghua Zong Mingxiao Zhang Shuai Li Wenqian Qi Juan Li Tonghua Liu Huijun Yang Chen Lu Xiaosong Hu |
| author_sort | Yonghua Zong |
| collection | DOAJ |
| description | Aim. The aim of this paper is to investigate the effects of ethyl pyruvate (EP) on experimental liver fibrosis induced by bile duct ligation (BDL) and explore the underlying molecular mechanisms. Material and Method. Rats were randomly divided into three groups: the sham group, the BDL group, and the BDL+EP group. Liver fibrosis was induced by common bile duct ligation and was evaluated by serum biochemical parameter levels, Masson’s trichrome staining, α-SMA expression, and collagen I deposition. The levels of Nrf2 signaling pathway-related antioxidant genes (Nrf2, SOD2, NQO1, and GSH-Px) in liver tissues were also measured. Meanwhile, the mRNA expression levels of HMGB1, IL-1β, TNF-α, and HSP27 were analyzed. In BDL-induced liver fibrosis rats, the successfully established model was confirmed by the significant increase of serum ALT and AST levels, the high liver fibrosis score, α-SMA expression, and collagen deposition. Results. Compared with the BDL group, EP administration could diminish fibrosis level and substantially increase the expression of Nrf2 signaling pathway-related antioxidant genes. Furthermore, EP significantly suppressed the mRNA expression levels of HMGB1, IL-1β, TNF-α, and HSP27. Conclusions. The results suggested that EP administration could effectively inhibit the liver fibrosis induced by BDL in rat, which may be associated with the enhanced activity of Nrf2 to mediate antioxidant enzyme system and downregulate the inflammatory genes. |
| format | Article |
| id | doaj-art-659942ea6bc0410bad3c70184fd96c1d |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-659942ea6bc0410bad3c70184fd96c1d2025-02-03T01:31:57ZengWileyGastroenterology Research and Practice1687-61211687-630X2019-01-01201910.1155/2019/29698022969802Effects of Ethyl Pyruvate on Bile Duct Ligation-Induced Liver Fibrosis by Regulating Nrf2 Pathway and Proinflammatory Cytokines in RatsYonghua Zong0Mingxiao Zhang1Shuai Li2Wenqian Qi3Juan Li4Tonghua Liu5Huijun Yang6Chen Lu7Xiaosong Hu8Luoyang Polytechnic, Luoyang 471000, ChinaLuoyang Polytechnic, Luoyang 471000, ChinaChengdu Medical College, Sichuan 610500, ChinaQingdao Binhai University, Qingdao 266555, ChinaChengdu Medical College, Sichuan 610500, ChinaTibet Traditional Medicine University, Lhasa 850000, ChinaChengdu Medical College, Sichuan 610500, ChinaUniversity of Electronic Science and Technology of China, Sichuan 610500, ChinaChengdu Medical College, Sichuan 610500, ChinaAim. The aim of this paper is to investigate the effects of ethyl pyruvate (EP) on experimental liver fibrosis induced by bile duct ligation (BDL) and explore the underlying molecular mechanisms. Material and Method. Rats were randomly divided into three groups: the sham group, the BDL group, and the BDL+EP group. Liver fibrosis was induced by common bile duct ligation and was evaluated by serum biochemical parameter levels, Masson’s trichrome staining, α-SMA expression, and collagen I deposition. The levels of Nrf2 signaling pathway-related antioxidant genes (Nrf2, SOD2, NQO1, and GSH-Px) in liver tissues were also measured. Meanwhile, the mRNA expression levels of HMGB1, IL-1β, TNF-α, and HSP27 were analyzed. In BDL-induced liver fibrosis rats, the successfully established model was confirmed by the significant increase of serum ALT and AST levels, the high liver fibrosis score, α-SMA expression, and collagen deposition. Results. Compared with the BDL group, EP administration could diminish fibrosis level and substantially increase the expression of Nrf2 signaling pathway-related antioxidant genes. Furthermore, EP significantly suppressed the mRNA expression levels of HMGB1, IL-1β, TNF-α, and HSP27. Conclusions. The results suggested that EP administration could effectively inhibit the liver fibrosis induced by BDL in rat, which may be associated with the enhanced activity of Nrf2 to mediate antioxidant enzyme system and downregulate the inflammatory genes.http://dx.doi.org/10.1155/2019/2969802 |
| spellingShingle | Yonghua Zong Mingxiao Zhang Shuai Li Wenqian Qi Juan Li Tonghua Liu Huijun Yang Chen Lu Xiaosong Hu Effects of Ethyl Pyruvate on Bile Duct Ligation-Induced Liver Fibrosis by Regulating Nrf2 Pathway and Proinflammatory Cytokines in Rats Gastroenterology Research and Practice |
| title | Effects of Ethyl Pyruvate on Bile Duct Ligation-Induced Liver Fibrosis by Regulating Nrf2 Pathway and Proinflammatory Cytokines in Rats |
| title_full | Effects of Ethyl Pyruvate on Bile Duct Ligation-Induced Liver Fibrosis by Regulating Nrf2 Pathway and Proinflammatory Cytokines in Rats |
| title_fullStr | Effects of Ethyl Pyruvate on Bile Duct Ligation-Induced Liver Fibrosis by Regulating Nrf2 Pathway and Proinflammatory Cytokines in Rats |
| title_full_unstemmed | Effects of Ethyl Pyruvate on Bile Duct Ligation-Induced Liver Fibrosis by Regulating Nrf2 Pathway and Proinflammatory Cytokines in Rats |
| title_short | Effects of Ethyl Pyruvate on Bile Duct Ligation-Induced Liver Fibrosis by Regulating Nrf2 Pathway and Proinflammatory Cytokines in Rats |
| title_sort | effects of ethyl pyruvate on bile duct ligation induced liver fibrosis by regulating nrf2 pathway and proinflammatory cytokines in rats |
| url | http://dx.doi.org/10.1155/2019/2969802 |
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